Hydroxylamine-O-sulphonic acid

Endpoint summary

Description of key information

Short term toxicity to fish:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Hydroxylamine-O-sulphonic acid on Zebra fish (Danio rerio) was determined to be 12.5 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

Short term toxicity to aquatic invertebrates:

1. After the exposure of test chemical with the shrimps for 48 hrs, lethal effect (LC50) were measured and determine to be 80-90 mg/l.

2. Based on the changes observed in the daphnia magna by the chemical exposure for 24 hrs, the EC50 was determine to be 32 mg/l.

3. Based on the immobility of daphnia magna by the chemical exposure for 48 hrs, the IC50 was determine to be 12 mg/l.

Based on the above data chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

Toxicity to aquatic algae and cyanobacteria:

After 72 hours of exposure to test item Hydroxylamine-O-sulphonic acid to various nominal test concentrations, EC50 was determine to be 152.78 mg/l graphically and through probit analysis.

Additional information

Summarized result for the determination of nature of chemical Hydroxylamine-O-sulphonic acid (2590-43-8) on the growth and other biological and physical activity of fish, aquatic invertebrates, algae and cyanobacteria when chemical comes in contact with test organisms, by considering the data for target as well as closely related read across chemicals which are as follows: 

 

Short term toxicity to fish:

Toxicity of chemical was determine and studied on the growth of fish (experimental study report 2018). This study was designed to assess the toxic effects of the test compound Hydroxylamine-O-sulphonic acid on the Zebra fish (Danio rerio). Test was conducted in compliance with the OECD guideline 203. The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 1 g of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous 1 hour stirring. After the completion of 1 hour stirring test solution was prepared for achieving the test concentration of 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. Study conducted under the static system for 96 hrs. Test animal was collected from Anytime Pet, Jariphataka, Nagpur. 8 fishes exposed to the test concentration. Aeration in test vessels was provided 1 day before the start of experiment. After the exposure of chemical (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 24 hours, 48 hours, 72 hours and 96 hours of the start of the experiment. Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for Hydroxylamine-O-sulphonic acid on Zebra fish (Danio rerio) was determined to be 12.5 mg/L by observing the mortality. Thus based on LC50 it can be concluded that the chemical was toxic and can be consider to be classified as aquatic chronic 3 as per the CLP classification criteria.

 

Short term toxicity to aquatic invertebrates:

 Short term toxicity of Hydroxylamine-O-sulphonic acid (2950 -43 -8) on the growth and other biological activity of aquatic invertebrates is predicted on the basis of it closely related read across chemicals. The studies are as mentioned below:

 

Short term toxicity study was performed to determine the toxic effect of the structurally and functionally similar read across chemicals on the invertebrates. Study was conducted to evaluate the toxicity of test chemical on the growth of shrimps. Study conducted under the static system in aerated water for 48 hrs. After the exposure of test chemical with the shrimps for 48 hrs, lethal effect (LC50) were measured and determine to be 80-90 mg/l. Based on the LC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

 

Similar study was conducted for another read across chemical. Aim of this study was to determine the action and effect of chemical when exposed with the daphnia magna. Study was conducted for 24 hrs under the static system. After the incubation period effect on 50 % population of test organism occurs were observed. Based on the changes observed in the daphnia magna by the chemical exposure for 24 hrs, the EC50 was determine to be 32 mg/l. Based on the EC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

 

In third weight of evidence study for structurally and functionally similar read across chemicals toxicity was measured. Aim of this study was to determine the action and effect of chemical when exposed with the daphnia magna. Test conducted in static system for 2 days. Based on the immobility of daphnia magna by the chemical exposure for 48 hrs, the IC50 was determine to be 12 mg/l. Based on the IC50, chemical was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.

 

Thus based on the overall studies for the structurally and functionally similar read across chemical for Hydroxylamine-O-sulphonic acid (2950 -43 -8), it can be concluded that the test chemical was toxic as value ranges from 12 -90 mg/l and classified as aquatic chronic 3 as per the CLP classification criteria.  

 

Toxicity to aquatic algae and cyanobacteria: 

This study was designed to assess the toxic effects of the test compound Hydroxylamine-O-sulphonic acid on the green alga Chlorella vulgaris (From experimental study report 2018). Test was conducted in compliance with the OECD guideline 201. Chemical was analytically moniorized by spectrophotometer. The test concentration chosen for the study were 6.25mg/l, 12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200mg/l. All the tests were carried out in 100mL conical flasks which were carefully autoclaved and sterilized. The test solution in each of these test vessels was kept constant which is 60 ml so that a sufficient amount of head space was left. The test item Hydroxylamine-O-sulphonic acid was prepared by adding 50 mg of test item in 250 ml of BBM to get the final concentration of 200mg/L. This stock solution was kept for stirring/sonication for 0 minutes to obtain a homogenous solution for the experiment. The test concentrations were chosen according to the available data of the test item. The concentrations chosen were set up to the water solubility limit. The remaining test solutions were prepared by dilution from the above stock solution. For the assessment of algal growth, the test was conducted in replicates. The control flask was maintained in triplicates as recommended in the OECD guideline and the test concentration were selected in geometric series which were maintained in duplicates. To obtain a quantitative concentration-response relationship by regression analysis, a linearizing transformation of the response data into probit was performed. Using the same, effective concentration (EC) were determined. After 72 hours of exposure to test item Hydroxylamine-O-sulphonic acid to various nominal test concentrations, EC50 was determine to be 152.78 mg/l graphically and through probit analysis. Based on the EC50, it can be consider that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Based on the overall effects on the growth and other behavior of aquatic species chemical Hydroxylamine-O-sulphonic acid was consider as toxic and classified as aquatic chronic 3 as per the CLP classification criteria.