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EC number: 228-762-1 | CAS number: 6358-09-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Effects on fertility
Description of key information
no data
Effect on fertility: via oral route
- Endpoint conclusion:
- no study available
Effect on fertility: via inhalation route
- Endpoint conclusion:
- no study available
Effect on fertility: via dermal route
- Endpoint conclusion:
- no study available
Effects on developmental toxicity
Description of key information
According to the result of the key study and to CLP criteria, the registered substance 2 -amino-6 -chloro-4 -nitrophenol was not classified for toxicity to reproduction and teratogenicity.
The NOAEL for developmental toxicity was established at ≥ 90 mg/kg bw.
Effect on developmental toxicity: via oral route
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- NOAEL
- 90 mg/kg bw/day
- Study duration:
- subacute
- Species:
- rat
- Quality of whole database:
- reliability 2
Effect on developmental toxicity: via inhalation route
- Endpoint conclusion:
- no study available
Effect on developmental toxicity: via dermal route
- Endpoint conclusion:
- no study available
Additional information
The developmental toxicity study of Chlororange (2-amino-6-chloro-4-nitrophenol) was conducted following methods comparable to the OECD Guideline 414 (Prenatal Developmental Toxicity Study).
Male and female rats were placed together for mating during the cohabitation period. The presence of spermatozoa in a vaginal smear was considered as Day 0 of gestation. Mated females were assigned randomly to the following treatment groups of 20 females each:
Group 1: Vehicle control (0.5% sodium carboxymethylcellulose)
Group2: 10 mg/kg bw/day
Group 3: 30 mg/kg bw/day
Group 4: 90 mg/kg bw/day
Aqueous 0.5% sodium carboxymethylcellulose served as the vehicle. The test substance was administered to mated female rats by oral gavage once daily from Days 5 through 15 of gestation.
The female rats were observed daily for clinical signs, abortions, premature deliveries and deaths. Body weights were recorded on Day 0 and at Days 5, 10, 15 and 20. Food consumption values were measured for day-intervals 0-5, 5-15, and 15-20 and for the entire study period (0-20).
All rats were sacrificed by CO2 asphyxiation on Day 20, and a macroscopic examination was performed. The number of corpora lutea in each ovary was recorded. The uterus of each rat was examined for pregnancy, number and distribution of implantations, live and dead foetuses, early and late resorptions, birth position (anterior/posterior), and placentae.
Each fetus was identified, weighed and examined for sex and gross external alterations. Approximately one-third of the fetuses in each litter were examined for soft tissue alterations and the remaining fetuses in each litter examined for skeletal alterations.
All females showed normal habits and behaviours throughout the study, and no animaldiedprior to scheduled sacrifice. Females of all dose groups had orange discolored urine throughout the application period at dose related intensity. Mean maternal bodyweight gain and mean food consumption was significantly reduced during the treatment phase in the females of the high dose group (90 mg/kg bw).
Gross necropsy did not reveal any organ alterations attributable to treatment.
No significant differences in the mean number of viable fetuses, the male to female fetal sex ratio, total bodyweights, birth position, number of runts, post-implantation losses, implantations, resorptions, uteri weights, placenta weights and corpora lutea between dosage groups and the control group were observed.
External, skeletal and visceral examinations of fetuses revealed minor variations (wavy ribs) at comparable intergroup frequencies with incidence within the spontaneous variation range of this strain of animals. 5 mid dose group fetuses and one high dose group fetus were found with head-neck edemas or whole-body edemas (anomalies) but since the incidence of this finding revealed no dose relation, it is considered to be coincidental.
Based on the above, administration of Chlororange (2-amino-6-chloro-4-nitrophenol) to female Crl:Wi/Br strain Wistar rats by oral gavage during gestation Days 5 to 15 at dose levels of 0, 10, 30 and 90 mg/kg bw resulted in a NOAEL of 30 mg/kg bw for maternal toxicity (significant reduction in body weight gain and mean food consumption).
The NOAEL for developmental toxicity was established at ≥ 90 mg/kg bw, the highest dose administered.
Justification for classification or non-classification
According to the result of the key study and to CLP criteria, the registered substance 2 -amino-6 -chloro-4 -nitrophenol was not classified for toxicity to reproduction and teratogenicity.
The NOAEL for developmental toxicity was established at ≥ 90 mg/kg bw.
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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