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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

Currently viewing:

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from peer reviewed journal

Data source

Reference
Reference Type:
publication
Title:
Safety of sodium fluorescein for in vivo study of skin
Author:
Ken-ichiro O’goshi and Jorgen Serup
Year:
2006
Bibliographic source:
Skin Research and Technology 2006; 12: 155–161

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: as per mentioned below
Principles of method if other than guideline:
Study is conducted to check the LD50 of Sodium fluorescein after oral administration in mice
GLP compliance:
not specified
Test type:
other: No data
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
EC Number:
208-253-0
EC Name:
Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
Cas Number:
518-47-8
Molecular formula:
C20H12O5.2Na
IUPAC Name:
disodium 3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diolate
Test material form:
other: Solid
Details on test material:
- Name of test material (IUPAC name): disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate
- Common name: Fluorescein sodium
- Molecular formula: C20H10Na2O5
- Molecular weight:376.274 g/mol
- Smiles notation: c1(c2c(oc3c1ccc(c3)[O-])cc(=O)cc2)c1c(cccc1)C(=O)[O-].[Na+].[Na+]
- InChl: 1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)25-18-10-12(22)6-8-16(18)19(15)13-3-1-2-4-14(13)20(23)24;;/h1-10,21H,(H,23,24);;/q;2*+1/p-2
- Substance type: Organic
Specific details on test material used for the study:
- Name of test material (as cited in study report):Sodium Fluorescein
- Molecular formula :C20H10O5.2Na
- Molecular weight :376.28 g/mol
- Substance type:Organic
- Physical state:Solid

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
no data available

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
not specified
Details on oral exposure:
No data available
Doses:
2200 mg/kg bw
No. of animals per sex per dose:
No data available
Control animals:
not specified
Details on study design:
no data available
Statistics:
No data available

Results and discussion

Preliminary study:
No data available
Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
2 200 mg/kg bw
Based on:
test mat.
Remarks on result:
other: 50 % mortality was observed
Mortality:
50% mortality was observed at dose 2200 mg/kg bw
Clinical signs:
other: No data available
Gross pathology:
No data available
Other findings:
No data available

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
the LD50 value were considered to be 2200 mg/kg,when mice were treated with sodium fluorescein orally.
Executive summary:

In a acute oral toxicity study, mice were treated with sodium fluorescein in the concentration of 2200 mg/kg bw. 50% mortality was observed at dose 2200 mg/kg bw.Therefore, LD50 value was considered to be 2200 mg/kg bw,when mice were treated with sodium fluorescein orally.