Pyridine-2-thiol 1-oxide, sodium salt

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPP 81-4 (Acute Eye Irritation)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Pyridine-2-thiol 1-oxide, sodium salt

EC Number:

223-296-5

EC Name:

Pyridine-2-thiol 1-oxide, sodium salt

Cas Number:

3811-73-2

Molecular formula:

C5H5NOS.Na

IUPAC Name:

sodium (1-oxo-1λ⁵-pyridin-2-yl)sulfanide

Details on test material:

- Analytical purity: 40%
- Lot/batch No.: 6RC190016

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

-Source: Ace Animals
Weight: 2.4kg

Test system

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

Amount: 0.1ml

Duration of treatment / exposure:

24h

Observation period (in vivo):

14d

Number of animals or in vitro replicates:

6

Details on study design:

-Scoring system: Draize Scale
-Examination times: 1h, 1, 2, 3, 7, 14 days

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

mean

Time point:

other: Throughout test

Score:

0

Max. score:

0

Reversibility:

other: not relevant

Results 2

Irritation parameter:

iris score

Basis:

mean

Time point:

other: Throughout study

Score:

1

Max. score:

1

Reversibility:

fully reversible

Remarks on result:

other: Cleared by day 2

Results 3

Irritation parameter:

conjunctivae score

Basis:

mean

Time point:

other: Throughout study

Score:

2

Max. score:

2

Reversibility:

fully reversible

Remarks on result:

other: Cleared by day 14

Other effects:

There was no corneal opacity noted at any observation period. Iritis, noted in 6/6 eyes, cleared by Day2. Conjuctival irritation, noted in 6/6 eyes, cleared by Day 14.
Three animals died by Day 2 with predeath signs of lethargy, prostration, soiling of the anogenital area, convulsions, tachypnea, ataxia, and wetness of the nose/mouth area. Physical signs noted in survivors included lethargy, yellow nasal discharge, and yellow staining of the nose/mouth area.
Necropsy of the animals that died during the study revealed abnormalities of the lungs, peritoneal cavity, and gastrointestinal tract, as well as wetness of the nose/mouth area and yellow nasal discharge.

Applicant's summary and conclusion

Conclusions:

The test article produced irritation which cleared within 14 days. However, the physical signs and mortality produced by the application indicates that the material is toxic to rabbits via this route. The information contained within this robust summary document comes from studies which are in the ownership of Arch Chemicals Inc. and which are protected in several regions globally. This information may not be used for any purpose other than in support of the Chemical safety Report submitted by Arch Chemicals Inc. under Regulation EC 1907/2006.