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EC number: 249-958-3 | CAS number: 29923-31-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994/03/23 - 1994/05/31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Remarks:
- Only four bacterial strains were tested. But these were two strains each with base pair substitution and frameshift mutation and thus did not influence the quality and validity of the study. Also a read-across study with an analogous substance was scientifically justified, because it was conducted from the disodium salt to the monosodium salt of the test substance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Only four bacterial strains were tested. But these were two strains each with base pair substitution and frameshift mutation and thus did not influence the quality and validity of the study.
- Principles of method if other than guideline:
- Only four bacterial strains were tested. But these were two strains each with base pair substitution and frameshift mutation and thus did not influence the quality and validity of the study
- GLP compliance:
- yes
- Remarks:
- Only four bacterial strains were tested. But these were two strains each with base pair substitution and frameshift mutation and thus did not influence the quality and validity of the study.
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- L-Glutamic acid, N-coco acyl derivs., monosodium salts
- EC Number:
- 269-087-2
- EC Name:
- L-Glutamic acid, N-coco acyl derivs., monosodium salts
- Cas Number:
- 68187-32-6
- IUPAC Name:
- 68187-32-6
Constituent 1
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Remarks:
- Only 4 bacterials strains were tested but there were 2 strains each with base pair substitution and frameshift mutation and this did not influence the quality and validity of the study
- Additional strain / cell type characteristics:
- not specified
- Remarks:
- Only 4 bacterials strains were tested but there were 2 strains each with base pair substitution and frameshift mutation and this did not influence the quality and validity of the study
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix (Aroclor 1254 induced)
- Test concentrations with justification for top dose:
- 0, 4, 20, 100, 500, 2500 and 5000 µg/plate (all doses were tested in triplicates)
- Vehicle / solvent:
- bi-distilled water
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2- aminoanthracene
- Remarks:
- TA 98, TA 100, TA 1535, TA 1537 with S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- TA 1537 without S9
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- TA 100, TA 1535 without S9
- Details on test system and experimental conditions:
- Test group
Top agar was prepared for the Salmonella strains by mixing 100 mL agar (0.6 % agar, 0.5 % NaCI) with 10 mL of a 0.5 mM histidine-biotin solution.
The following ingredients were added (in order) to 2 mL of molten top agar at approx. 45 °C:
0.1mL of an overnight nutrient broth culture of the bacterial tester strain
0.1 mL test compound solution
0.5 mL S-9 Mix (if required) or buffer
After mixing, the liquid was poured into a petridish with minimal agar (1.5 % agar, Vogel-Bonner E medium with 2 % glucose). After incubation for approximatly 48 hours at approx. 37 °C in the dark, colonies (his* revertants) were counted.
Two independent experiments were performed.
Positive controls
Positive control plates were included for each strain. The following substances were used as positive controls.
a) without metabolic activation:
Sodium-azide: TA 100, TA 1535 9-Aminoacridine: TA 1537 2-Nitrofluorene: TA 98
b) with metabolic activation:
2-Aminoanthracene: TA 98, TA 100, TA 1535, TA 1537
Negative controls
a) solvent controls (0 microgram/plate)
b) untreated controls - Evaluation criteria:
- A test article is classified mutagenic if either of the following conditions under a) and b) is achieved:
a) a test article produces at least a 2-fold increase in the mean number of revertants per plate of at least one of the tester strains over the mean number of revertants per plate of the appropriate vehicle control at complete bacterial background lawn
b) a test article induces a dose-related increase in the mean number of revertants per plate of at least one of the tester strains over the mean number of revertants per plate of the appropriate vehicle control in at least two to three concentrations of the test article at complete bacterial background lawn.
The test results must be reproducible.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Remarks:
- Only 4 bacterials strains were tested but there were 2 strains each with base pair substitution and frameshift mutation and this did not influence the quality and validity of the study
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- .TEST-SPECIFIC CONFOUNDING FACTORS
- Water solubility: test substance did not precipitate on the plates up to the highest investigated dosis
- Precipitation: no
RANGE-FINDING/SCREENING STUDIES: The substance proved to be toxic for the bacterial strain TA 100 in the absence of a metabolizing system at a dose of 5000µg/plate only in the cytotoxic experiment. Therefore 5000 µg/plate was chosen as the highest dose in the experiment. - Remarks on result:
- other: All strains/cells were tested. Only four bacterial strains were tested. But these were two strains each with base pair substitution and frameshift mutation and thus did not influence the quality and validity of the study.
Any other information on results incl. tables
Only four bacterial strains were tested. But these were two strains each with base pair substitution and frameshift mutation and thus did not influence the quality and validity of the study.
Applicant's summary and conclusion
- Conclusions:
- Based on the results of the read across study, the substance can be considered as not mutagenic in S. typhimurium with and without matabolic activation
- Executive summary:
The test substance was investigated for its mutagenicity in accordance with OECD guideline 471 in test strains TA 98, TA 100, TA 102, TA 1537 and TA 1538 in two independant experiments. None of the test strains showed an increased mutation rate at any test concentration. The respective positive controls were valid. The test substance is considered to be non mutagenic in this test system.
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