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Diss Factsheets
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EC number: 201-744-0 | CAS number: 87-41-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study (with GLP and analytics)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Phthalide
- EC Number:
- 201-744-0
- EC Name:
- Phthalide
- Cas Number:
- 87-41-2
- Molecular formula:
- C8H6O2
- IUPAC Name:
- 1(3H)-Isobenzofuranone
- Details on test material:
- - Name of test material (as cited in study report): Phthalide
- Analytical purity: about 100%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- -Method: The test solution was prepared by adding the test item to the test media using an ultra-turrax without any further pre-treatment. Then, the fish were placed into the test aquaria.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish (Danio rerio)
- Source: Hoechst Marion Russel Germany, GmbH
- Age at study initiation: about 4 months
- Length at study initiation (length definition, mean, range): 2.8 cm (2.5 - 3.2 cm)
- Weight at study initiation (mean and range): 0.24 g (0.1-0.4 g)
- Feeding during test: Withdrawal of food 1 day before and during exposure
ACCLIMATION
- Acclimation period: about 10 days
- Health during acclimation (any mortality observed): mortality of 1% during last 14 days
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 22 °C
- pH:
- 7.9-8.4
- Dissolved oxygen:
- 4.5-9.1 mg/L
- Nominal and measured concentrations:
- nominal (mg/L): 0, 46.4, 100, 315, 464, 1000;
measured, after 1 hour (mg/L): below limit of detection, 42.5, 85.4, 205.0, 381.9, 773.3;
measured, after 96 hours (mg/L): below limit of detection, 39.2, 82.2, 190.3, 423.6, not determined. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: All glass aquaria (30 x 22 x 24 cm), Volume of water: 10 L
- No aeration
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.24 g fish/L test water
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city of Frankenthal, not chlorinated and passed through a charcoal filter, aerated.
- pH about 8.0-8.6
- Ca/Mg ratio: (90 mg/L)/(10 mg/L)
OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime
EFFECT PARAMETERS MEASURED:
observations: mortality and symptoms after 1, 4, 24, 48, 72, and 96 h
measurements: pH, oxygen and temperature after 1, 24, 48, 72 and 96 h - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 82 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 190 - < 424 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: Symptoms of intoxication: Narcotic-like state, apathy (oserved at concentrations of 190 mg/L and above).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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