diethylmethoxyborane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study. Since the hydrolysis of the substance is slow, the daily renewal of the test solution is considered sufficient to keep the test concentration within an acceptable range. No effects were observed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

425-380-9

EC Name:

-

Cas Number:

7397-46-8

Molecular formula:

C5H13BO

IUPAC Name:

methyl diethylborinate

Details on test material:

- Name of test material (as cited in study report): DEMB
- Physical state: liquid
- Analytical purity: 99.4%
- Stability under test conditions: No, hydrolyses slowly
- Storage condition of test material: At room temperature in the dark

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
The test solutions originated from those prepared for the acute fish toxicity test (NOTOX Project 157736).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Age at study initiation: < 24 hours
- Method of breeding: With newborn animals, i.e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel.
- Feeding during test: none

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/L CaCO3

Test temperature:

20.8 - 21.0 °C

pH:

7.4 - 7.9

Dissolved oxygen:

6.3 - 9.2 mg/L

Nominal and measured concentrations:

100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL, all-glass
- Fill volume: 80 mL medium
- Aeration: No aeration of the test solution.
- Renewal rate of test solution (frequency): Test solutions were renewed after 24 hours of exposure.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
- Biomass loading rate: 10 per vessel containing 80 mL medium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ultrapure water (tap water purified by reverse osmosis (milli-R0) and subsequently passed over activated carbon and ion-exchange cartridges: Milli-Q water; Millipore Corp., Bedford, Mass., USA)
- Intervals of water quality measurement: temperature daily, pH and dissolved oxygen at the start and at the end of the test

OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Mobility, at 24 hours and 48 hours.

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: no effects observed

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

- Under the conditions of the present test, DEMB induced na effects in Daphnia magna exposed to 100 mg/L
- Analytical support was not available as detection of the concentrations tested was not possible with any of the analytical methods available. Hence, no sampling for analysis was performed.

Results with reference substance (positive control):

The actual responses in this reference test are within the ranges of the expected responses at the different concentrations.

Immobility at the different test concentrations:
0.00 mg/L: 0%
0.10 mg/L: 0%
0.18 mg/L: 0%
0.32 mg/L: 0%
0.56 mg/L: 20%
1.0 mg/L: 80%
1.8 mg/L: 100%