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EC number: 202-430-6 | CAS number: 95-54-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Remarks:
- As per ICCA HPV review conducted by Haskell.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: 40 CFR Part 797.1330 Daphnid chronic toxicity test. Environmental effects testing guidelines.
- GLP compliance:
- yes
Test material
- Reference substance name:
- o-phenylenediamine
- EC Number:
- 202-430-6
- EC Name:
- o-phenylenediamine
- Cas Number:
- 95-54-5
- Molecular formula:
- C6H8N2
- IUPAC Name:
- benzene-1,2-diamine
- Details on test material:
- - Purity: 99.8%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Solutions for quantitative analysis were taken from controls and selected test solutions at 0, 7, 14, and 21 days of the study. Two replicates per concentration were selected using random numbers on each sample day.
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Nominal and measured concentrations:
- Nominal: 0.0066, 0.016, 0.041, 0.1, 0.26, 0.64, and 1.6 mg/L
Measured: Lowest concentration was below detection limit, 0.0027, 0.021, 0.065, 0.18, 0.54, and 1.5 mg/L, respectively - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Pyrex beakers 250 mL; 6.1 cm diameter
- Aeration: Unaerated
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 10
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.065 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.54 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.18 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.67 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- mortality
- Remarks on result:
- other: 0.57-0.83 = 95% fiducial interval
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.46 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- reproduction
- Remarks on result:
- other: 0.33-0.77 = 95% fiducial interval
- Reported statistics and error estimates:
- For length: Jonckherre's trend test and Kruskal-Wallis and Dunn's multiple comparison test.
Any other information on results incl. tables
Mean Measured Concentration (mg/L) |
Percent Adult Survival |
1stReprod Day |
Total Young |
Young/Surv. Parent |
Immobilized Young |
Length (mm) |
Control |
93 |
8.0 |
399 |
105 |
0 |
4.5 |
0.0066 |
88 |
8.0 |
340 |
93 |
0 |
4.5 |
0.0027 |
95 |
8.0 |
355 |
93 |
0 |
4.5 |
0.021 |
95 |
8.1 |
372 |
96 |
0 |
4.5 |
0.065 |
85 |
8.1 |
324 |
93 |
0 |
4.4 |
0.18 |
95 |
8.0 |
319 |
82 |
0 |
4.3 |
0.54 |
55 |
12 |
64 |
30 |
3.5 |
3.8 |
1.5 |
0 |
D |
D |
D |
D |
D |
D = all dead
Applicant's summary and conclusion
- Conclusions:
- This study established a 21-day EC50 (mortality) and EC50 (reproduction) for o-phenylenediamine in daphnids of 0.67 and 0.46 mg/L, respectively. The NOEC established by this study was 0.065 mg/L o-phenylenediamine.
- Executive summary:
Nominal test concentrations of oPDA were 0.0066, 0.016, 0.041, 0.10, 0.26, 0.64 and 1.6 mg/L. The lowest concentration (0.0066 mg/L) was below the present limit of quantitation and was not analyzed. The remaining mean, measured concentrations were 0.0027, 0.021, 0.065, 0.18, 0.54, and 1.5 mg/L, respectively. The number of adults surviving to day 21 was statistically significantly reduced at 0.54 mg/L and above. The total number of young and the total number of young produced per surviving adult in 21 days was statistically significantly reduced at 0.18 mg/L and above. Length was statistically significantly reduced at 0.065 mg/L and above using Jonckheere's trend test. However, length at 0.065 mg/L was not statistically significant using Kruskal-Wallis and Dunn's multiple comparison test. In addition, length at 0.065 mg/L was not considered biologically significant based on the slight decrease in average measurement compared to control, the variability between replicates, and the lack of precision in measuring daphnid length. The 21-day EC50, based on adult survival and nominal concentrations, was 0.67 mg/L. The 21-day EC50, based on the total number of young produced per surviving adult in 21 days and nominal concentrations was 0.46 mg/L. The 21-day NOEC was 0.065 mg/L and 21 -day MATC was 0.11 mg/L, based upon adult reproduction and length.
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