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EC number: 203-836-6 | CAS number: 111-12-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study completed on 16 May 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Methyl oct-2-ynoate
- EC Number:
- 203-836-6
- EC Name:
- Methyl oct-2-ynoate
- Cas Number:
- 111-12-6
- Molecular formula:
- C9H14O2
- IUPAC Name:
- methyl oct-2-ynoate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- For determination of the appropriate test concentrations for the main test, a range-finding test with nominal test item concentrations of 0.25, 2.5 and 25 mg/L was performed. For this, three replicates per treatment were tested. The test medium of the highest concentration of 25 mg/L was stirred until dissolution for 1 hour at room temperature in the dark. The test media of the two lower concentrations were prepared in a series of further dilution steps.
For the determination of the actual test item concentrations, duplicate samples were taken from each treatment at the start of the test.
After 24 hours and at the end of the test (after 72 hours), stability samples (containing algae) were taken in duplicate from all test concentrations and from the control.
The stability samples at 24 hours could not be taken from the test vessels itself, as the principle of a closed system is, that the test vessels have to remain completely filled with test medium during the entire test period. Therefore, for this sampling at 24 hours, a set of additional flasks containing the corresponding test medium (with algae) was incubated under conditions identical to the test.
For sampling at the end of the test, the test medium of the treatment replicates was pooled.
All samples were stored frozen (at -20 ± 5 °C) immediately after sampling. Based on pre-experiments for investigation of the storage stability, the test item was found to be stable in the test water under these storage conditions (see Analytical Procedure, Appendix 1).
The concentrations of FOLIONE were analytically measured in one of the duplicate samples taken from the control and all test concentrations from the start, 24 hours and end of the test.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Based on the results of the range-finder, the following nominal concentrations of FOLIONE were selected for the main test: 0.068, 0.15, 0.33, 0.72, 1.6 and 3.5 mg/L, applying a spacing factor of 2.2. The main test was performed in a closed system. Additionally, a control (test water without test item) was tested in parallel.
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
The test was started using a nominal algal cell density of 5000 cells/mL. The initial cell density was selected according to the recommendations of the OECD test guideline. The algal cell density in the pre-culture was determined using an electronic particle counter (Coulter Counter, Model Z2).
Study design
- Test type:
- static
- Water media type:
- other: Reconstituted test water (AAP Medium) prepared according to the test guidelines was used for algal cultivation and testing. Analytical grade salts were dissolved in sterile purified water.
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 15 mg/L as CaCO3
- Test temperature:
- The water temperature during the test was maintained at 22 °C.
- pH:
- The pH of the test media was in the range of 7.5 to 7.8 during the test period.
- Nominal and measured concentrations:
- The measured concentrations of FOLIONE in the test media of the nominal test concentrations of 0.068 to 3.5 mg/L were between 82 and 85% of the nominal values at the start of the test. Thus, the correct dosage of the test item could be verified. During the test period, the test item concentrations in the test media decreased and were between
The Time-Weighted mean measured concentrations determined over the course of the 72 hour exposure were 0.012, 0.063, 0.13, 0.30, 0.88 and 2.4 mg/L, respectively, at the 0.068, 0.15, 0.33, 0.72, 1.6 and 3.5 mg/L (nominal) test concentrations. - Reference substance (positive control):
- yes
- Remarks:
- Potassium DiChromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.79 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI: 0.75 - 0.85 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.2 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CI: 0.18 - 0.23 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.063 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.32 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: 95% CI: 0.30 - 0.33 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.12 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Remarks on result:
- other: 95% CI: 0.11 - 0.13 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.012 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Yield
- Results with reference substance (positive control):
- For evaluation of the algal quality and experimental conditions, potassium dichromate is tested as a positive control twice a year to demonstrate satisfactory test conditions. The 72-hour EC50 for growth rate in the reference test IES Study Number 20160277 was 0.9 mg/L (October 2016) and showed that the sensitivity of the test system was within the range recommended by the guideline (72-hour EC50 for the growth rate 0.9 - 1.5 mg/L).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Executive summary:
A clear concentration-response relationship was observed for both biological endpoints growth rate and yield during the exposure period.
Based on the results of the study, the :
EC50 (growth rate - 72 hr) = 0.79 mg/L
EC10 (growth rate - 72 hr) = 0.20 mg/L
NOEC (growth rate - 72 hr) = 0.063 mg/L
EC50 (yield - 72 hr) = 0.32 mg/L
EC10 (Yield - 72 hr) = 0.12 mg/L
NOEC (Yield - 72 hr) = 0.012 mg/L
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