Pyrazole

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: guideline study (no analytics, no GLP)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: DIN 38 412

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Pyrazole

EC Number:

206-017-1

EC Name:

Pyrazole

Cas Number:

288-13-1

Molecular formula:

C3H4N2

IUPAC Name:

1H-pyrazole

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

- Method: The test solution was prepared by adding the test item to the test media without any pre-treatment. Then, the fish were placed into the test aquaria.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe (Leuciscus idus)
- Source: Fischzucht Paul Eggers, 2354 Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range): 8.4 cm (range 7.5 - 9.3 cm)
- Weight at study initiation (mean and range): 6.0 g (range 4.4 - 7.6 g)
- Feeding during test: Withdrawal of food one day before and during exposure

ACCLIMATION
- Health during acclimation (any mortality observed): 0%

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol/L

Test temperature:

20 to 21 °C

pH:

7.1 to 7.5

Dissolved oxygen:

7.9 to 8.7 mg/L

Nominal and measured concentrations:

0, 1000, 2150, 4640 and 10000 mg/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: All glass aquaria (30 x 22 x 24 cm), Volume of water: 10 L
- Aeration: Continuous aeration with oil-free air, slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 6 g fish/L test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412, Part II, 1982
- Alkalinity: pH about 8.0
- Ca/Mg ratio: 4 : 1


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
observations: mortality and symptoms after 1, 4, 24, 48, 72, and 96 h
measurements: pH and oxygen content after 1, 24, 48, 72 and 96 h

Reference substance (positive control):

yes

Remarks:

Chloroacetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

ca. 2 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

- Behavioural abnormalities: Symptoms of intoxication: Narcotic-like state, Tumbling [LOEC: 2150 mg/L]

Results with reference substance (positive control):

- LC50: about 31 mg/L (after 48 h)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes