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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Analytical data missing

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: internal company guideline
Deviations:
not specified
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
p-phenylenediamine
EC Number:
203-404-7
EC Name:
p-phenylenediamine
Cas Number:
106-50-3
Molecular formula:
C6H8N2
IUPAC Name:
benzene-1,4-diamine
Details on test material:
Nako H is aquivalent to p-Phenylenediamine (see NCI)
The test substance was pre-desolved in the test-water and afterwards applicated to the test basins.
pH, O2 and temperature was determined before adding test material and checked after 2, 24, 48, 72 and 96 h

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Leuciscus idus melanotus
Details on test organisms:
ex Paul Egger, 2345 Hohenwestedt

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
de-chlorinated water (activ-carbon filter), hardness 17.2 and 17.0 °d
carbonate hardness 11 and 9 °d (Aquamerck, E. Merck, Darmstadt)
Test temperature:
20 +/- 1°C
temperature was tested directly after adding the substance and 2, 24, 48,72 and 96 h afterwards with Hg-thermometer
pH:
8.1 - 8.7 (Digital-ph-Meter 650, Knick, Elektronische Meßgeräte, Berlin)
Dissolved oxygen:
100 ml air/min, O2-content > 7mg/l
O2 was tested directly after adding the substance, 2, 24, 48,72 and 96 h afterwards with Oxi 39 (Wissenschaftlich-Technische-Werkstätten GmbH, Weilheim, Obb.)
O2-content 7.8 - 8.7 mg/l
Details on test conditions:
light/dark rythm 12/12 h, light from the top, light density directly over the tank approx. 700 Lux
Fishes were added to the water min. 14 days at 20 °C before adding test substance. 65 h before beginning of the test 10 fishes per tank (20l) were added.

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 0.1 - <= 1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: no data
Details on results:
highest tested concentration without mort. 0.1 mg/l at 48 and 96 h
lowest tested concentration on 100 % mort. 1 mg/l at 48 and 96 h
fishes died within 24 h after starting the test.
The group in concentration between 0.01 and 0.1 mg/lg didn't show any difference to control group

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
LC50 = 0.1 to 1 mg/l