Reaction product of 3,9-dibromobenzanthrone condensed with 2 equivalents of 1-aminoanthraquinone, subsequently further condensed under oxidative conditions

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 October 1992 to 06 November 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Test concentrations: 0 / 22 / 50 / 100 / 220 / 500 mg/L
The substance concentration was determined in all tested concentration groups after 0, 48 and 96 hours (in the 500 mg/L group only the value at study start was determined).

Vehicle:

no

Details on test solutions:

Water for dilution was added to the test substance, the mixture was homogenized using an Ultra-Turrax and Ultrasonic Bath and poured into the chamber under stirring with a glass rod.The test batches were present as dark red solutions. No segregation or formation of precipitates could be observed.In order to determine the substance concentration, water samples (approx. 20 mL) were taken from the middle of the test chamber using a screw-neck glass bottle at study start, and after 48 and 96 hours.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Species: zebrafish (Brachydanio rerio) (HAMILTON-BUCHANAN)
Origin: Central Toxicology (Hoechst AG)Date of hatching: December 15, 1991
Delivery date: May 14, 1992
The fish were kept for 14 days in water for dilution before the start of the study under the following conditions:
Temperature: 22 ± 1 °C
Oxygen content: > 80 % of the saturation value
Duration of light period: 12 hours daily
Density: < 1 g fish / L water
Feeding: twice daily ad libitum
Food: Tetra Min, Tetra Werke, Melle
Prior to study start the body length of 10 representative fish from each batch was measured. These animals were not used during the study.
Mean length: 2.6 cm to 3.2 cm

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

No post exposure observation

Hardness:

No data

Test temperature:

21.0 - 22.3 °C

pH:

7.0 - 7.8

Dissolved oxygen:

6.1 - 10.1 mg/L

Salinity:

Not applicable.

Conductivity:

No data

Nominal and measured concentrations:

Nominal concentrations: 0 / 22 / 50 / 100 / 220 / 500 mg/L
Measured concentrations: The values determined showed a slight decrease of the test concentrations over 96 hours but were all in a range of +/-20% of the theoretical value.

Details on test conditions:

Water for dilution
Reconstituted water, composition according to ISO/DIS 7346/1 was used as water for dilution.
Preparation was carried out in a unit consisting of two Hostalen®- lined steel vessels with a capacity of 1700 liters each
The dilution water was prepared as described and was aerated until oxygen saturation. The pH was measured before the use of each study batch. It was in a range of 8.3 - 8.5.
Test conditions
The study was conducted in a static system. The test chambers, which were calibrated to 10 liters, were made of glass (length 30 cm, width 22 cm, height 24 cm) and stood in a water bath made of Hostalit Z® with a Plexiglas viewing panel. The temperature of the water bath was regulated by a thermostat to 22 + 1 °C. The chambers were illuminated from above from 06.00 a.m. to 06.00 p.m. The light intensity directly over the chambers was approximately 700 lux.The test chambers were not aerated during the course of the study.
After the test concentration had been prepared and water parameters recorded, 10 fish were assigned at random to each test and control chamber. The fish received feed for the entire study period. Inspection of the fish took place after 2 - 4, 24, 48, 72 and 96 hours and involved recording the mortality and visible alterations in appearance and behaviour. Dead fish were removed from the chambers. Fish were considered dead when there was a lack of opercular movement and no response to slight mechanil stimulus. The water parameters were measured and recorded at the same dates.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

220 mg/L

Conc. based on:

test mat.

Basis for effect:

behaviour

Details on results:

Recording of symptoms was disturbed by the dark red discoloration of the test water. The 220 mg/L group showed no effect in comparison with the control. The fish of the 500 mg/L group showed changes in behaviour and swimming behaviour.

Results with reference substance (positive control):

Not applicable.

Reported statistics and error estimates:

No data

Sublethal observations / clinical signs:

0 mg/L (Control groups)

Hours after study start	3	6	24	48	72	96
Lethality (absolute)	0/21	0/21	0/21	0/21	0/21	0/21
No changes in appearance and behaviour	21/21	21/21	21/21	21/21	21/21	21/21

 

22 mg/L

Hours after study start	3	6	24	48	72	96
Lethality (absolute)	0/7	0/7	0/7	0/7	0/7	0/7
Recording of symptoms disturbed	x	x	x	x	x	x
No changes in appearance and behaviour	a	a	a	a	a	a

 

50 mg/L

Hours after study start	3	6	24	48	72	96
Lethality (absolute)	0/7	0/7	0/7	1/7	2/7	3/7
Recording of symptoms disturbed	x	x	x	x	x	x
No changes in appearance and behaviour	a	a	a	a	a	a

 

100 mg/L

Hours after study start	3	6	24	48	72	96
Lethality (absolute)	0/7	0/7	0/7	0/7	1/7	1/7
Recording of symptoms disturbed	x	x	x	x	x	x
No changes in appearance and behaviour	a	a	a	a	a	a

 

220 mg/L

Hours after study start	3	6	24	48	72	96
Lethality (absolute)	0/7	0/7	0/7	0/7	0/7	0/7
Recording of symptoms disturbed	x	x	x	x	x	x
No changes in appearance and behaviour	a	a	a	a	a	a

 

500 mg/L

Hours after study start	3	6	24	48	72	96
Lethality (absolute)	0/7	0/7	0/7	0/7	0/7	0/7
Recording of symptoms disturbed	x	x	x	x	x	x
No changes in appearance and behaviour	a	a	a	a	a	a

a = all fish

Validity criteria fulfilled:

yes

Conclusions:

In this 96-hour acute toxicity study with the test material, only one fish died at 500 mg/L . No mortality occurred in the 220 mg/L group. The LC50 after 48 and 96 hours therefore was > 500 mg/L.

Executive summary:

Indanthren-Oliv HT Colloisol was tested for acute toxicity in zebra fish (Brachydanio rerio) over a total of 96 hours according to the method, in a static test system. Because no lethality occurred at 220 and 500 mg/L the LC50 after 96 hours exceeds 500 mg/L.

The concentrations tested were 22 / 50 / 100 / 220 / 500 mg/L and a negative control (0 mg/L).

All tested concentrations were present as black discolorations.

The substance concentration was determined in all tested concentration groups after 0, 48 and 96 hours (in the 500 mg/L group only the value at study start was determined). The values determined show a slight decrease of the test concentrations over 96 hours but were all in a range of +/- 20 % of the theoretical value.

The test concentrations were present as stable suspensions in test water. The observed adverse effects to fish were not related to the concentration of the test substance. Lethality was observed in the 50 mg/L group (3/7 fish) and in the 100 mg/L group (1/7 fish) but not in the other tested groups. Therefore it can be assumed, that the effects are not caused by the soluble fraction. Mechanical or other physical effects of Indanthren-Oliv HT Colloisol were not reproducible.

So it is not possible to find out a LC50 based on these effects.

Recording of symptoms was disturbed because of the discoloration of the test water by the test substance.

Description of key information

Key value determined in a GLP accredited laboratory study performed in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test) EU Method C.1 (Acute Toxicity for Fish).

According to this test, the test item does not have toxic effects on fish.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

500 mg/L

Additional information

A GLP study was performed with the substance in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test) and EU Method C.1 (Acute Toxicity for Fish). Lethality was observed in the 50 mg/L group (3/7 fish) and in the 100 mg/L group (1/7 fish) but not in the other tested groups; no lethality occurred at 220 and 500 mg/L. The test concentrations were present as stable suspensions in test water. The substance concentration was determined in all tested concentration groups after 0, 48 and 96 hours (in the 500 mg/L group, only the value at study start was determined). The values determined show a slight decrease of the test concentrations over 96 hours but were all in a range of +/- 20 % of the theoretical value. The observed adverse effects to fish were not considered to be related to the concentration of the test substance. Therefore, it can be assumed, that the effects are not caused by the soluble fraction. Mechanical or other physical effects of the test were not reproducible. As no lethality occurred at 220 and 500 mg/L, the LC50 after 96 hours exceeds 500 mg/L.