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EC number: 255-138-6 | CAS number: 40910-49-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Jan - March 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study done to guideline, in compliance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3-(1-ethoxyethoxy)-3,7-dimethylocta-1,6-diene
- EC Number:
- 255-138-6
- EC Name:
- 3-(1-ethoxyethoxy)-3,7-dimethylocta-1,6-diene
- Cas Number:
- 40910-49-4
- Molecular formula:
- C14H26O2
- IUPAC Name:
- 1-[(3,7-dimethylocta-1,6-dien-3-yl)oxy]-1-ethoxyethane
- Test material form:
- gas under pressure: refrigerated liquefied gas
- Details on test material:
- Chemical name (IUPAC) 3-(1-ethoxyethoxy)-3,7-dimethylocta-1,6-diene
CAS Number 40910-49-4
EC number 255-138-6
Molecular formula C14H26O2
Molecular weight 226.40 g/mol
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Nominal and measured concentrations:
- Nominal: 1.0, 3.2, 10, 32 and 100% of a water soluble fraction (saturated solution).
Initial measured concentrations: 0.18, 0.60, 2.4, 8.5 and 29 mg/l
Time-weighted average concentrations: 0.099, 0.13, 0.28, 2.0 and 6.7 mg/l - Details on test conditions:
- The test item was known to be volatile and hence testing was conducted in sealed test vessels in order to minimize possible losses due to volatilization. In order to prevent inhibition of growth due to the restriction of gaseous exchange, additional sodium bicarbonate was added to the culture mediumto provide a source of carbon dioxide for algal growth.
- Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 12 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL 9.6-16
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 2.3 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL 1.6-2.9
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.3 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks on result:
- other: 95% CL 2.5-4.2
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.85 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Remarks on result:
- other: 95% CL 0.35-1.3
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.28 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Inhibition of both growth rate and yield
- Details on results:
- During the exposure period the measured concentrations decreased to 1-30% of initial at the end of the test. The decrease in test concentrations may be related to one or more or a combination of factors, like for instance: photosensitivity of the test substance, adsorption of the test substance to algal cells, chemical instability of the test substance. Given these results, the EC50 was based on the TWA concentrations that were calculated as 0.099, 0.13, 0.28, 2.0 and 6.7 mg/l.
- Results with reference substance (positive control):
- A reference test was carried out to check the sensitivity of the test system used by WIL Research Europe to Potassium dichromate (Merck, Art. 1.04864, Batch K44879664). Closed test vessels were used as to mimic testing a volatile test article and results were compared with standard ‘open’ systems.
The EC50 for growth rate inhibition (72h-ERC50) was 1.3 mg/l with a 95% confidence interval ranging from 1.2 to 1.5 mg/l. The historical ranges for growth rate inhibition using standard ‘open’ systems range between 0.82 and 2.3 mg/l. Hence, the 72h-ERC50 for the present test in closed systems is within the historical range.
The EC50 for yield inhibition (72h-EYC50) was 0.50 mg/l with a 95% confidence interval ranging from 0.47 to 0.53 mg/l. The historical ranges for yield inhibition using standard ‘open’ systems lie between 0.43 and 1.1 mg/l. Hence, the 72h-EYC50 for the present test in closed systems was within range.
It was concluded that the sensitivity of algae exposed in closed test systems towards Potassium dichromate was not significantly different from the sensitivity of algae exposed in standard ‘open’ reference studies during the past years.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study with Pseudokirchneriella subcapitata, Elintaal inhibited both growth rate and yield of this fresh water algae species significantly at TWA concentrations of 2.0 mg/l and higher.
The EC50 for growth rate inhibition (72h-ERC50) was 12 mg/l with a 95% confidence interval ranging from 9.6 to 16 mg/l. The corresponding EC10 was 2.3 mg/L (95% CL 1.6 to 2.9 mg/L).
The EC50 for yield inhibition (72h-EYC50) was 3.3 mg/l with a 95% confidence interval ranging from 2.5 to 4.2 mg/l. The corresponding EC10 was 0.85 mg/L (95% CL 0.35 to 1.3 mg/L).
The 72h-NOEC for inhibition of both growth rate and yield was 0.28 mg/l.
The preferred observational end point in this study is algal growth rate inhibition because it is not dependent on the test design (ECHA guidance Chapter R.7b v1.1) and classification shall be based on the ErC50 (EU CLP regulation No 1272/2008 and its adaption 286/2011). In terms of the NOEC and EC10 values, there is a preference for use of the EC10 value because it is derived from the dose response curve. In contrast the NOEC depends on the experimental design (e.g. the concentrations used in the test). Thus the 72-h EC50 of 12 mg/L and EC10 of 2.3 mg/L based on growth rate are used for classification purposes.
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