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EC number: 223-098-9 | CAS number: 3734-67-6
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Jahir Alam Khan (2011) studied the biodegradation of Azo Dye by Moderately Halotolerant Bacillus megaterium.
The standard inoculums (25 ml) was used to inoculate 225 ml mineral salts basal media in which 15 ml of the dye solution has been added after autoclaving. The inoculated media was then incubated in shaking incubator at 150 rpm. Percentage dye degradation was determined spectrophotometrically every alternate day for twenty days.
Percentage dye degradation by the isolated Bacillus megaterium was found to be 64.89% in 20 days. Under the test conditions, we can conclude that Red 2G is inherently biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
In the key study, Jahir Alam Khan (2011) studied the biodegradation of Azo Dye by Moderately Halotolerant Bacillus megaterium.
The standard inoculums (25 ml) was used to inoculate 225 ml mineral salts basal media in which 15 ml of the dye solution has been added after autoclaving. The inoculated media was then incubated in shaking incubator at 150 rpm. Percentage dye degradation was determined spectrophotometrically every alternate day for twenty days.
Percentage dye degradation by the isolated Bacillus megaterium was found to be 64.89% in 20 days. Under the test conditions, we can conclude that Red 2G is inherently biodegradable
In a supporting studies by Lata Kumari et al. (2016), BHUSSp X2 microbial strain was isolated from contaminated sites of carpet cluster Bhadhoi. The bacteria were used for the decolourization of effluent from dying plants of the cluster in Uttar Pradesh, India.
Within 24 hours, upto 97 % decolourization could be achieved at pH 8, temperature 35 °C under static condition, and initial concentration of 200 mg/l.
Similarly, under shaking conditions (100 rpm), upto 45 % decolorization could be achieved at pH 8, temperature 35 °C within 24 hours and initial concentration of 200 mg/l. This is possibly due to reduced activity of azoreductase with increase dissolved oxygen level under shaking conditions.
It can be concluded that under the test conditions RED 2G was found to be inherently biodegradable.
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