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Diss Factsheets
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EC number: 413-090-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 April 2006 to 28 April 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Principles of method if other than guideline:
- The method is based on that described by ETAD (Ecological and Toxicological Association of the Dyestuffs Manufacturing Industry), in which activated sludge obtained from a sewage treatment plant is used as the microbial source.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Details on sampling:
- The test item and reference substance concentrations are not confirmed by analytical methods; they are based on nominal concentrations.
- Vehicle:
- no
- Details on test solutions:
- Concentration reference substance (3.5-Dichlorophenol, mg/L): 5, 10, 20
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Type: mixed population of aquatic microorganisms (activated sludge)
Origin: aeration tank of a domestic sewage treatment plant (Municipal STP Cologne-Stammheim)
Date of collection: 2006-04-18
Pretreatment: aeration of the activated sludge, daily fed with synthetic medium
pH of the suspension before application: 7.8
Synthetic sewage feed: A synthetic sewage feed is made by dissolving the following amounts of substance in 1 litre of water.
16.0 g peptone
11.0 g meat extract
3.0 g urea
0.7 g NaCl
0.4 g CaCl2 2H2O
0.2 g MgSO4 7H20
2.8 g K2HOP4
pH of the synthetic sewage feed: 7.1 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- No post exposure observation period
- Hardness:
- No data
- Test temperature:
- 21.3-21.7 °C
- pH:
- 7.8-8.1
- Dissolved oxygen:
- 2.5-4.5 mg/L
- Salinity:
- Not applicable.
- Nominal and measured concentrations:
- Test concentrations (mg/L): 100, 1000, 10000 mg/L
Test item concentration in physico-chemical oxygen consumption control (mg/L) : 10000 - Details on test conditions:
- Type of application: direct weighing
Test concentration of the activated sludge: 1200 mg/L suspended solids
Test temperature: 20 + 2° C
Stirring period of test item before start of incubation time: 16 hours
Incubation time: 3 hours with permanent aeration
The physico-chemical oxygen consumption control is carried out, since some substances may consume oxygen by chemical reactivity, in order to be able to differentiate between physico-chemical oxygen consumption and biological oxygen consumption (respiration), at least the maximum concentration of the test item is additionally tested without activated sludge. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The physico-chemical oxygen consumption has been determined at 10000 mg/l test substance concentration. As no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations.
- Results with reference substance (positive control):
- Reference substance : 3.5-Dichlorophenol
Test concentration [mg/1] Respiratory rate [mg/1 . h] Inhibition [%]
5 24.0 23.8
10 18.0 42.9
20 8.4 73.3
Reference compound
Reference compound name: 3.5 Dichlorophenol (Aldrich)
Purity: 97 %
Batch number: 15809KI-392
Comments: The reference substance concentrations result from the quantities used in the preparations and have not been confirmed by analytical methods - Reported statistics and error estimates:
- None reported.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Reaktiv Gelb F68072 FW showed 17.5 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/L.
EC50 > 10000 mg/L - Executive summary:
A study was performed to assess the toxicity of Reaktiv Gelb F68072 FW to bacteria.
The study was in accordance with the Commission Directive 88/302/EEC Part C. 11.This test method is in most parts identical with OECD Guideline 209. Study conducted in compliance with the Principles of Good Laboratory Practice (GLP).
The activated sludge was exposed to Reaktiv Gelb F68072 FW at different concentrations. The respiration rate of each mixture was determined after aeration periods of 3 hours.
Reaktiv Gelb F68072 FW showed 17.5 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/L.
The EC50 is higher than 10000 mg/L.
Reference
Test item : Reaktiv Gelb F68072 FW
Test concentration [mg/1] |
Respiratory rate test item [mg/1 • h] |
Phys.-chem. O2consumption [mg/1 • h] |
Respiratory rate - phys.-chem. O2consumption [mg/1 • h] |
Inhibition [%] |
100 |
30.0 |
*0.0 |
30.0 |
4.8 |
1000 |
34.0 |
*0.0 |
34.0 |
0.0 |
10000 |
26.0 |
0.0 |
26.0 |
17.5 |
Control, mean |
31.5 |
|
||
Control 1 |
30.0 |
|
||
Control 2 |
33.0 |
|
Comments relevant to result:
Test concentrations are given as nominal concentrations and have not been confirmed by analytical methods.
* The physico-chemical oxygen consumption has been determined at 10000 mg/l test substance concentration. As no physico-chemical oxygen consumption was observed at that test item concentration this observation also holds true for the lower test item concentrations
Description of key information
The EC50 is higher than 10000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
Additional information
A study was performed to assess the toxicity of Reaktiv Gelb F68072 FW to bacteria.
The study was in accordance with the Commission Directive 88/302/EEC Part C. 11.This test method is in most parts identical with OECD Guideline 209. Study conducted in compliance with the Principles of Good Laboratory Practice (GLP).
The activated sludge was exposed to Reaktiv Gelb F68072 FW at different concentrations. The respiration rate of each mixture was determined after aeration periods of 3 hours.
Reaktiv Gelb F68072 FW showed 17.5 % respiration inhibition of activated sludge at a test item concentration of 10000 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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