1,7-Naphthalenedisulfonic acid, 2-[[4-chloro-6-(cyanoamino)-1,3,5-triazin-2-yl]amino]-5-hydroxy-6-[2-[2-methoxy-5-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-, lithium sodium salt (1:?:?)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Oct. 12, 1999 to Nov. 08, 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Analysis of the only test concentration plus a control at test start and after 48 h of exposure.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method of administration: Direct weighing

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna STRAUS, parthenogenetic females
- Source: Laboratory bred at Bayer AG Leverkusen
- Age at study initiation (mean and range, SD): 0-24 h

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

None

Post exposure observation period:

No

Hardness:

14.5 °dH

Test temperature:

20.1 °C

pH:

8.0-8.1

Dissolved oxygen:

8.5-8.6 mg/L (48 h)

Nominal and measured concentrations:

Nominal: 0 and 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Cylindrical test vessels
- Material, size, headspace, fill volume: diameter 4.0 cm, height 6.5 cm, 20 mL test medium
- Aeration: No
- Renewal rate of test solution: No
- No. of organisms per vessel: 10 daphnids/vessel
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Keeping water: M4-Medium according to Elendt and BGA (1992)
- Method of administration: Direct weighing

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-Medium according to Elendt and BGA


OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Number of immobile daphnids after 24 h and 48 h

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 100 mg/L

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 105.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 68.6 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

- Other biological observations: No
- Mortality of control: No

Table 1. Study Results

After 24 h Nominal concentration	After 48 h Arithmetic mean of analytical values
EC0:≥100 mg/L	≥ 68.6 mg/L organic components
1800	≥ 68.6 mg/L (test substance; including inorganic components)

 

The above test result refers to a concentration of the test substance which was calculated directly from analytically determined TOC values. According to the relevant product information, 1 mg/L TOC equals to 2.8 mg/L of the organic components of the test substance (structural formula: C23H21CIN6014S4; molecular weight 769.16 g/mol (free acid).

As these organic components correspond to 65 % of the molecular weight of the test substance, 100 mg/L of the test Substance equals to 23 mg/L of organic C. Thus, the above analytical values indicate a very good recovery rate.

Validity criteria fulfilled:

yes

Conclusions:

Under the test conditions, the 48 h EC0 value of the test substance (including inorganic components) was determined to be 105.5 mg/L.

Executive summary:

A study was performed to assess the acute toxicity of test substance to Daphnia magna under static conditions. The study was conducted in accordance with EU Method C.2. in compliance with GLP.

 

Daphnids were exposed to a limit test concentration of 100 mg/L of test substance dissolved in water. Analysis was performed for the test concentration plus a control at the test start and after 48 h of exposure.

Observations were made on the immobilisation rate, after 24 and 48 h of exposure.

 

Under the test conditions, the 48 h EC0 value of the test substance (including inorganic components) was determined to be 105.5 mg/L.

Description of key information

The 48 h EC0 value of the test substance was determined to be 105.5 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L

Additional information

A study was performed to assess the acute toxicity of test substance to Daphnia magna under static conditions. The study was conducted in accordance with EU Method C.2.

Daphnids were exposed to a limit test concentration of 100 mg/L of test substance dissolved in water. Analysis was performed for the test concentration plus a control at the test start and after 48 h of exposure.

Observations were made on the immobilisation rate, after 24 and 48 h of exposure.

The 48 h EC0 value of the test substance (including inorganic components) was determined to be 105.5 mg/L (Dr. Caspers, 1999).