Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 274-427-8 | CAS number: 70210-31-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06.06. – 08.06. 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- Adopted July 22, 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- The stock solution was prepared by dissolution of 5.0 g of the test substance in 1000 mL deionized water on the day of the testing.The concentrations 10, 100 and three replicates of 1000 mg·L-1 of the test substance were tested in the preliminary test.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- TEST SYSTEM- Laboratory culture: The activated biological sludge containing mixed culture of microorganisms obtained from the second step of sewage treatment plant of Pardubice was used for the testing. The wastewater processed by the sewage treatment plant is predominantly municipal.PREPARATION OF THE INOCULUMThe activated sludge was collected two days before the day of testing. After the sample collection the sludge was washed with potable water for 30 minutes and subsequently decanted for 30 minutes. This procedure was repeated three times in total.Further the sludge was fed with the 100 mL synthetic sewage feed per 2 L of diluted sludge suspension at permanent aeration till the day of the test. The suspended solids concentration was determined from 10 mL of sludge suspension after 30-minutes sedimentation.Before the test the sludge was suspended in water up to concentration about 3000 mgof suspended solids per litre. The pH adjustment to 6.1 was carried out. In that way modified sludge suspension was aerated until the use.JUSTIFICATION FOR THE SELECTION OF THE TEST SYSTEMThe activated sludge used in the test is in conformity with the recommendations of the test guidelines.
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- approx. 7 – 8
- Nominal and measured concentrations:
- 10, 100 and three replicates of 1000 mg·L-1 (Z1 – Z5 preliminary test) The level of inhibition of oxygen consumption by the test substance in the preliminary test was lower than 50 % and demonstrated that a definitive test is unnecessary.5, 10 and 20 mg·L-1 (R1 – R3 reference substance)Further two blank controls, the three abiotic controls with the highest concentration of the test substance and two beakers with specific nitrification inhibitor to determine the nitrification potential of sludge were included.
- Details on test conditions:
- TEST SYSTEM- Test vessel: oxygen vessels (BOD bottles) with volume of about 280 mL for measurement of oxygen concentration- Type (delete if not applicable): closed- Aeration: by filtered pressured air- No. of vessels per concentration (replicates): 1- No. of vessels per control (replicates): 2- Sludge dose on concentration of 3000 mg.L-1 : 211 mL.L-1WATER PARAMETERS- deionized waterOTHER TEST CONDITIONS- Adjustment of pH: the pH adjustment to 6.1 was carried out. - Photoperiod: daily lightingTEST PROCEDUREThe defined dose of the test or reference substance, 100 mL of deionized water, 16 mL of the synthetic sewage feed and 250 mL of the prepared the sludge suspension were introduced into each test container. The volume was replenished up to 500 mL with deionized water. The control samples were prepared only from the same volume of nutrient medium and sludge and replenished up to 500 mL with water. The check of abiotic decomposition was performed at the highest concentration of the test substance and nutrient medium without sludge and with addition of 1 mL of mercury chloride solution.EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The decline of oxygen concentration was measured and recorded in 30-seconds intervals for 11 minutes.TEST CONCENTRATIONS- Spacing factor for test concentrations: This inhibition of oxygen consumption by the test substance in the preliminary test was lower than 50 % and demonstrated that a definitive test is unnecessary. Range finding study- Test concentrations: 10, 100 and 1000 mg.L-1 Results with positive control:- Results with reference substance valid? yes- Relevant effect levels: EC50 = 7.5 mg·L-1 (95% confidence interval: 3.0 – 18.4 mg·L-1)
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid? yes - Relevant effect levels: EC50 = 7.5 mg·L-1 (95% confidence interval: 3.0 – 18.4 mg·L-1)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The highest inhibition the respiration rate of the activated sludge caused by the test substance Direct Blue 26 was 15.6 % in the preliminary test. This low level of inhibition of oxygen consumption by the test substance in the preliminary test demonstrated that a definitive test is unnecessary. The result could be used as final one, because preliminary test design includes three replicates required by guidelines.Exact value of EC50 could not be calculated, the value of EC50 is given in the form of a range: EC50 > 1 000 mg·L-1
- Executive summary:
The influence of the test substance, Direct Blue 85, on the respiration rate of activated sludge was investigated after a contact time of 3 hours.
Test performance
Test was performed according to:
OECD Test Guideline No. 209 – Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation). Adopted July 22, 2010
Method C.11 – Activated Sludge Respiration Inhibition Test (Carbon and Ammonium Oxidation), Council Regulation (EU) No. 2016/266, published in O.J. L 54/1, 2016.
The preliminary test was performed using 3 concentrations 10, 100 and 1000 mg·L-1. Further two blank controls, the three abiotic controls with the highest concentration of the test substance was included and two beakers with specific nitrification inhibitor to determine the nitrification potential of sludge.
3,5-dichlorophenol was used as reference substance. Three concentrations in geometric progression with a factor of 2.0 (5, 10 and 20 mg·L-1) were selected.
The abiotic decomposition was not detected even at the highest concentration of the test substance.
The pH value during the preliminary test was in the range of 7.0 to 7.8.
Validity of the test
The prescribed validity criteria in the test were fulfilled. Since all criteria of acceptability were met, this study is considered to be valid.
Results of the respiration inhibition test
The highest inhibition the respiration rate of the activated sludge caused by the test substance Direct Blue 26 was 15.6 % in the preliminary test. This low level of inhibition of oxygen consumption by the test substance in the preliminary test demonstrated that a definitive test is unnecessary.
The result could be used as final one, because preliminary test design includes three replicates required by guidelines.
Exact value of EC50 could not be calculated, the value of EC50 is given in the form of a range: EC50 > 1 000 mg·L-1
Reference
Description of key information
The highest inhibition the respiration rate of the activated sludge caused by the test substance Direct Blue 26 was 15.6 % in the preliminary test. This inhibition of oxygen consumption by the test substance in the preliminary test was lower than 50 % and demonstrated that a definitive test is unnecessary. Exact value of EC50 could not be calculated, the value of EC50 is given in the form of a range: EC50 > 1 000 mg·L-1
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.