1-(3,5-dihydroxyphenyl)-2-[[2-(4-hydroxyphenyl)-1-methylethyl]amino]ethan-1-one hydrobromide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 November 1999 to 05 January 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Version / remarks:

1992

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Version / remarks:

1992

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.3110 (Ready Biodegradability)

Version / remarks:

1998

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-(3,5-dihydroxyphenyl)-2-[[2-(4-hydroxyphenyl)-1-methylethyl]amino]ethan-1-one hydrobromide

EC Number:

222-148-7

EC Name:

1-(3,5-dihydroxyphenyl)-2-[[2-(4-hydroxyphenyl)-1-methylethyl]amino]ethan-1-one hydrobromide

Cas Number:

3371-33-3

Molecular formula:

C17H19NO4.BrH

IUPAC Name:

1-(3,5-dihydroxyphenyl)-2-{[1-(4-hydroxyphenyl)propan-2-yl]amino}ethan-1-one hydrobromide

Test material form:

solid: particulate/powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
activated sludge from a domestic waste water treatment plant supplied by sewage plant Groß-Zimmern, Germany
- Preparation of inoculum for exposure:
The activated sludge used for this study was washed by centrifugation and supernatant liquid phase was decanted. The solid material was resuspended in tap water and again centrifuged. This procedure was repeated twice. An aliquot of the final sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined. Based on this ratio, calculated aliquots of washed sludge suspension, corresponding to 4 g dry material per litre were mixed with test water and then areated until use. Before use the sludge was filtered through cotton wool.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

3.26 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

5.2 mg/L

Based on:

ThOD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: grade salts were added to deionised water to give stock solutions. Stock solutions were diluted in deionised water, the test medium was aerated for 20 minutes and allowed to stand for about 20 hours at the test temperature. The dissolved oxygen concentration was 8.6-8.7 mg/L at about 22°C.
- Test temperature: 22°C
- pH: 7.5 - 7.6
- Aeration of dilution water: yes

TEST SYSTEM
- Culturing apparatus: Karlsruher flasks of 250 ml volume with glass stopper
- Number of culture flasks/concentration: 16 bottles containing the test substance and inoculum; 16 bottles containing the reference compound Aniline and inoculum (procedure control); 16 bottles containing only inoculum (inoculum control); 10 bottles containing the test substance, reference compound Aniline and inoculum (toxicity control).
- Method used to create aerobic conditions: inoculum and medium solutions were sufficiently aerated prior to the start of the test.
- Measuring equipment: oxygen electrode (WTW Oxi 340)
- Test performed in closed system: yes


SAMPLING
- Sampling frequency: Oxygen measurements were performed in duplicate on days 0, 2, 5, 7, 12, 14, 21 and 28 after the start of the test

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

other: based on ThOD (NH4)

Value:

33

Sampling time:

28 d

Details on results:

The residual oxygen concentration in the test flasks did not drop below 0.5 mg O2/L at any time. The difference of duplicate values for the degradation of the test substance at the plateau, at the end of the test and at the end of the 10-day window was less than 20%.

BOD5 / COD results

Results with reference substance:

The reference compound Aniline was sufficiently degraded to in the mean 72% after 14 days, and to 97 after 28 days of incubation. The percentage biodegradation of the reference compound confirms the suitability of the used activated sludge inoculum.

Any other information on results incl. tables

In the modified Closed Bottle test 3.26 mg/L of the test item were tested, corresponding to a ThOD of about 5.2 mgO2/L.

Since all acceptability criteria prescribed by the protocol were met, this study was considered to be valid.

Percentage biodegradation (based on ThOD_NH4) at different time intervals during the exposure period of 28 days

Treatment	Conc. [mg/L]	flask No.	% biodegradation after n days of exposure
			2	5	7	12	14	21	28
Test substance	3.26	1a	6	10	5	19	24	27	35
		1b	6	10	7	15	28	31	31
		mean	6	10	6	17	26	29	33
Procedure Control (aniline)	2.0	2a	2	54	55	66	72	95	95
		2b	2	54	55	68	72	95	98
		mean	2	54	55	67	72	95	97
Toxicity control	Test substance: 3.2  Aniline: 2.12	4a	n. d.	n. d.	38	n. d.	52	63	65
		4b	n d.	n. d.	37	n. d.	52	63	65
		mean	n. d.	n. d.	38	n. d.	52	63	65

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Under the test conditions in the mean 33% biodegradation of TH 1165 Keton II was determined during the test period of 28 days (based on ThOD(NH4)).
The percentage biodegradation did not exceed 60% within the 10-day window. The test substance can therefore not considered to be ready biodegradable.