(E)-3-methyl-5-cyclopentadecen-1-one

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 2001-09-13 to 2001-12-19

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Experiment conducted under GLP, following OECD Guidelines. No deviations were observed.

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

Principles of method if other than guideline:

Not applicable.

GLP compliance:

yes (incl. QA statement)

Remarks:

Inspected on 28 February 2000. Signed on 26 April 2000

Analytical monitoring:

no

Details on sampling:

- Concentrations: 100, 180, 320, 560, and 1000 mg/kg
- Sampling method: At the start of the test, 10 earthworms were placed in each test and control vessel on the artificial soil, at random. The test vessels were covered with plastic film with ventilation holes and maintained in a temperature controlled room at 21°C under continuous illumination at 548 to 720 lux. The earthworms were not individually identified and received no food during exposure. Any mortalities and sub-lethal effects of exposure were recorded 7, and 14 days after the start of treatment. The criterion of death was taken to be the absence of any reaction to a physical stimulus at either end of the body. Any unaccounted for worms were classed as mortalities.
- Sample storage conditions before analysis: none

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil (if used): Amount of test material were each separately incorporated into 2.5 kg (dry weight) of artificial soil with 650 mL of deionised reverse osmosis water and mixed using a Hobart QE 200 mixer to give the test concentrations with a nominal moisture content of 30% of dry weight. The control was prepared in an identical manner without the addition of test material.
- Controls: An amount of soil was taken for moisture content determination from the control and each test group. The remaining soil was dispersed to four replicates R1-R4.

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworms
- Source: Blades Biological, Edenbridge (Kent, UK)
- Age at test initiation (mean and range, SD): no data
- Weight at test initiation (mean and range, SD): 0.38 +/- 0.07 g

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Remarks:

none

Post exposure observation period:

no data

Test temperature:

21°C

pH:

5.7-6.0

Moisture:

27-30 % of dry weight

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 1 L glass beaker
- Amount of soil or substrate: 0.5 kg dry weight of substrate
- No. of organisms per container (treatment): 10 earthworms
- No. of replicates per treatment group: 1
- No. of replicates per control: 1
- No. of replicates per vehicle control: not applicable


OTHER TEST CONDITIONS
- Photoperiod: 24:0
- Light intensity: 483-705 lux

Nominal and measured concentrations:

- nominal concentrations: 0, 100, 180, 320, 560, and 1000 mg/Kg
- No measured concentration.

Reference substance (positive control):

yes

Remarks:

chloroacetamide (Sigma Batch No 70K0053)

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

250 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL

Remarks:

240-260 mg/kg

Duration:

14 d

Dose descriptor:

other: Highest concentration without observed effect

Effect conc.:

180 mg/kg soil ww

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

not determinable

Details on results:

no data

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: 14d-LC50 of 23 mg/Kg (95% confidence limits = 20 -25 mg/Kg).
- Other: none

Reported statistics and error estimates:

no data

Table 6.3.1/1: Cumulative mortality in the definitive test.

Nominal concentration (mg/Kg)	Cumulative mortality (initial population : 40)		% Mortality
	Day 7	Day14	Day 7	Day 14
Control	0	0	0	0
100	0	0	0	0
180	0	0	0	0
320	38	38	95	95
560	40	40	100	100
1000	40	40	100	100

Validity criteria fulfilled:

yes

Conclusions:

The 14d-LC50 for the test material to earthworms based on nominal test concentrations was 250 mg/Kg (95% confidence limits = 240 -260 mg/Kg).

Executive summary:

A study, according to OECD Guideline 207 and EEC Commission Directive 87/302/EEC, was performed to assess the acute toxicity of the test material to the earthworm (Eisenia foetida) in an artificial soil.

Following a preliminary range-finding test (test concentrations of 10, 100 and 1000 mg/Kg), earthworms were exposed in groups of 40 (4 replicates of 10 worms per concentration) to a range of concentrations of 100, 180, 320, 560 and 1000 mg/Kg of soil for a period of 14 days. The number of mortalities was determined after 7 and 14 days exposure.

A positive control study using chloroacetamide, conducted approximately every 6 months, was reported for reference purposes.

The 14d-LC50 for the test material to earthworms based on nominal test concentrations was 250 mg/Kg (95% confidence limits = 240 -260 mg/Kg).

The result of the positive control study gave a 14d-LC50 for chloroacetamide of 23 mg/Kg (95% confidence limits = 20 -25 mg/Kg).

Description of key information

OECD Guideline 207, GLP, key study, validity 1:

14d-LC50 (Eisenia fetida) = 250 mg/Kg (95% confidence limits = 240 -260 mg/Kg) based on nominal concentrations

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

250 mg/kg soil dw

Additional information

One key study is available, according to OECD Guideline 207 and EEC Commission Directive 87/302/EEC, to assess the acute toxicity of the registered substance to the earthworm (Eisenia fetida) in an artificial soil.

Following a preliminary range-finding test (test concentrations of 10, 100 and 1000 mg/Kg), earthworms were exposed in groups of 40 (4 replicates of 10 worms per concentration) to a range of concentrations of 100, 180, 320, 560 and 1000 mg test substance/Kg of soil for a period of 14 days. The number of mortalities was determined after 7 and 14 days exposure.

A positive control study using chloroacetamide, conducted approximately every 6 months, was reported for reference purposes. The result of the positive control study gave a 14d-LC50 for chloroacetamide of 23 mg/Kg (95% confidence limits = 20 -25 mg/Kg).

The 14d-LC50 for the test material to earthworms based on nominal test concentrations was 250 mg/Kg (95% confidence limits = 240 -260 mg/Kg).