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EC number: 269-085-1 | CAS number: 68187-30-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001-01-15 to 2001-01-31
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with acceptable restrictions. No coefficient of variation was reported for the control for both endpoints (growth rate/biomass).
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples of the 7.76, 37.6 and 182 mg/L treatments were taken after 0, 24, 48 and 72 h.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A clear stock solution with a nominal concentration of 2 g product/L deionized water was prepared. Appropriate volumes were taken from the constantlly stirred solution and pipetted into the test vessels. 10 mL of a 10-fold concentrate of the test medium were pipetted to each test vessel and then each test solution was filled up to a final volume of 100 mL with deionized water. - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Source: Pflanzenphysiologisches Institut der Universittät Göttingen, delivered on 2000-09-21
ACCLIMATION
- Acclimation period: Preincubation about 3-4 days in test medium
- Culturing media and conditions: same as test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24.5 °C
- pH:
- after 0 h: 7.8
after 72 h: 7.9 - 8.5 - Nominal and measured concentrations:
- Nominal concentration: 0, 3.53, 7.76, 17.1, 37.6, 82.6, 182, 400 mg product/L.
The base analysis yielded in 186 mg TOC/g product resulting in 0, 0.66, 1.44, 3.18, 6.99, 15.36, 33.85, 74.4 mg TOC/L. Three of the eight concentrations (1.44, 6.99 and 33.85 mg TOC/L) were determined via TOC-Analysis. Mean measured concentrations were 1.42, 6.5 and 28.4 mg TOC/L with a mean substance recovery of 91.9 %. - Details on test conditions:
- TEST SYSTEM
- Test vessel: conical flasks
- Material: glass; Size: 300 mL; Fill volume: 100 mL
- Initial cells density: 10^4 cells/mL
- Control end cells density: 1.1x10^6 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes, according to guideline
TEST MEDIUM / WATER PARAMETERS
- Source of dilution water: deionized water
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH was measured daily and the temperature range was recorded
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity and quality: daylight spectrum with approximately 2000 lux
EFFECT PARAMETERS MEASURED: Algal cell concentrations were measured after 24, 48 and 72 h test duration.
- Determination of cell concentrations: Coulter-Counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2 - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 32.56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks on result:
- other: converted to active matter
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 11.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks on result:
- other: converted to active matter
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 38 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 110 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 750 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Reported statistics and error estimates:
- Effect concentrations were calculated using probit method. A t-test was performed to obtain to EC0.
- Validity criteria fulfilled:
- yes
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 32.56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
Referenceopen allclose all
After 0, 24, 48 and 72 hours samples were taken from nominal concentrations of 7.76 mg/L, 37.6 mg/ and 182 mg/L (according to the base analysis which yielded in 186 mg TOC/g product: 1.44, 6.99 and 33.85 mg TOC/L, respectively). Mean measured concentrations were 1.42, 6.5 and 28.4 mg TOC/L. The mean substance recovery was 91.9 % of the nominal values, therefore the nominal concentrations were taken into account for the calculation of the EC-values.
Description of key information
EC50 (72h) = 32.56 mg/L, NOEC (72h) = 11.25 mg/L (nominal, based on active matter) for Scenedesmus subspicatus (OECD 201, read across)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 32.56 mg/L
- EC10 or NOEC for freshwater algae:
- 11.25 mg/L
Additional information
No studies investigating the toxicity of l-Glutamic acid, N-coco acyl derivs., disodium salts (CAS No. 68187-30-4) to algae are available. Thus, available data for the source substance l-Glutamic acid, N-coco acyl derivs., monosodium salts (CAS No. 68187-32-6) was used in a read across approach. The read across is considered valid as both substances are structurally identical (solely differing in the degree of deprotonation being di- and monosodium salts, respectively), which assume very similar physico-chemical properties resulting in identical environmental behavior and ecotoxicity.
The read across key study was performed according to GLP and OECD guideline 201 using Scenedesmus subspicatus as test organism (Cognis, 2001). A 29.6 % solution of the substance in water was tested. Nominal concentrations between 3.53 and 400 mg/L (based on test material) were tested over a period of 72 h. The 72h-EC50 (based on active matter) is determined to be 32.56 mg/L and the 72h-NOEC is determined to be 11.25 mg/L. Due to the very high structural similarity, this result can also be considered for l-Glutamic acid, N-coco acyl derivs., disodium salts (CAS No. 68187-30-4).
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