Registration Dossier
Registration Dossier
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EC number: 700-413-2 | CAS number: 6331-96-0
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
During 72 h the test substance showed no toxic effects against algae at a limit test concentration of 100 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
A study was performed to assess the adverse effects of 2-amino-4,5-dichlorobenzenesulfonic acid on the growth rate (= rate of increase in cell density with time) and the yield (= biomass at time t minus initial biomass) of the planktonic freshwater algal species Desmodesmus subspicatus (former name: Scenedesmus subspicatus) over several generations. The study was conducted in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 ‘Freshwater Alga and Cyanobacteria, Growth inhibition test’ (2009) which is equivalent to OECD Guideline for Testing of Chemicals No. 201 (2006). Exponentially growing algal cells were exposed for a period of 72 hours to a limit test concentration of nominally 100 mg/L of 2-amino-4,5-dichlorobenzenesulfonic acid dissolved in dilution water. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and a folded filter. The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate (index r), relative to control cultures grown under identical conditions. No toxic effects against algae were observed at a limit test concentration of 100 mg/L. The validity criteria of the test were met.
The result should read as >100 mg/L.
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