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EC number: 229-176-9 | CAS number: 6422-86-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 8 days
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Reliable without restriction; study followed acceptable scientific principles.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 005
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Five pregnant Sprague-Dawley rats were exposed from gestation day 12 through gestation day 19 to di (2-ethylhexyl) terephthalate at 500 mg/kg bw/day. On gestation day 19, all females were euthanized by carbon dioxide inhalation and a laprohysterectomy was performed. Fetuses were weighed, anogential distance obtained, and fetuses were decapitated. After decapitation, fetuses were sexed and the right and left testes were removed and snap-frozen in liquid nitrogen until gene expression analysis was carried out. Global gene expression in the fetal testis was determined for gene pathways involved in: cholesterol transport and steroidogenesis, intracellular lipid and cholesterol homeostasis, insulin signaling, transcriptional regulation, oxidative stress, alpha inhibin (essential for normal Sertoli cell development), and genes involved in communication between Sertoli cells and gonocytes.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Bis(2-ethylhexyl) terephthalate
- EC Number:
- 229-176-9
- EC Name:
- Bis(2-ethylhexyl) terephthalate
- Cas Number:
- 6422-86-2
- Molecular formula:
- C24H38O4
- IUPAC Name:
- 1,4-bis(2-ethylhexyl) benzene-1,4-dicarboxylate
- Reference substance name:
- Reference substance 001
- Cas Number:
- 6422-86-2
- Details on test material:
- -Name as cited in publication: diethylhexyl phthalate (DEHP)
Constituent 1
Constituent 2
Results and discussion
Any other information on results incl. tables
-Anogenital Distance:
Anogenital distance was not significantly different in male fetuses exposed to di (2-ethylhexyl) terephthalate (1.6344 ± 0.03) relative to control (1.61 ± 0.02).
-Microarray analysis and gene ontology:
A heat map was generated to compare the expression level of 391 genes between the treatment group relative to a universal mean. The list of 391 significant genes contained 225 unknown and uncharacterized transcribed sequences, which were not considered in the classification. The remaining 167 genes were grouped into the following categories: related to lipid, sterol, and cholesterol homeostatis (31 genes); related to lipid, sterol, and cholesterol transport (10 genes); steroidogenesis (12 genes); transcription factors (9 genes); signal transduction (22 genes); oxidative stress (11 genes); and cytoskeleton-related (13 genes).
-Quantitation of gene expression by real-time RT-PCR:
None of the genes from animals exposed to di (2-ethylhexyl) terephthalate were changed in a statistically significant manner compared to controls.
Applicant's summary and conclusion
- Conclusions:
- Five pregnant Sprague-Dawley rats were exposed from gestation day 12 through gestation day 19 to di (2-ethylhexyl) terephthalate at 500 mg/kg bw/day. On gestation day 19, all females were euthanized by carbon dioxide inhalation, fetuses were weighed, and anogential distance was obtained. Fetuses were sacrificed, sexed, and the right and left testes were removed. Genes associated with pathways involving lipid, sterol, and cholesterol transport, steroidogenesis, intracellular lipid and cholesterol homeostasis, oxidative stress, insulin signaling, and transcriptional regulation, were evaluated in the present study using Real-time Quantitative Reverse Transcription-Polymerase Chain Reactions. A total of 18 genes were investigated. No statistically significant alterations were noted in any of these genes in animals exposed to di (2-ethylhexyl) terephthalate.
Based on an absence of adverse effects on major gene pathways that allow for normal male reproductive tract development, di (2-ethylhexyl) terephthalate is not expected to be classified for “Germ Cell Mutagenicity” according to GHS. - Executive summary:
In the present study, five pregnant Sprague-Dawley outbred CD rats were exposed to di (2-ethylhexyl) terephthalate from gestation day 12 through 19 at 500 mg/kg bw/day. Ten pregnant Sprague-Dawley rats were also administered the vehicle (corn oil) and served as the controls. After administering the last dose on gestation day 19, all dams were euthanized by carbon dioxide asphyxiation and a laprohysterectomy was performed. Fetuses were removed, weighed, anogenital distance obtained, sexed, and the testes were removed from male fetuses. Anogenital distance was not significantly altered in male fetuses exposed to the test substance and none of the genes representing major gene pathways that allow for normal male reproductive tract development were altered by exposure to di (2-ethylhexyl) terephthalate.
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