Octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable study report which meets basic scientific principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Principles of method if other than guideline:

Five males and five females were administered intraperitoneal with the test substance at dose levels of 0, 500 and 1000 mg/kg bw. All animals were observed for 14 days post-treatment.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate

EC Number:

218-216-0

EC Name:

Octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate

Cas Number:

2082-79-3

Molecular formula:

C35H62O3

IUPAC Name:

octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl)propionate

Details on test material:

- Physical state: solid

Test animals

Species:

rat

Strain:

other: Tif:RAIf

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: CIBA-GEIGY LTD. Tierfarm, 4334 Sisseln, Switzerland
- Age at study initiation: 7-8 weeks
- Weight at study initiation: male 192-202 g, female 182-184 g
- Housing: 5 per cage
- Diet (e.g. ad libitum): Rat food, NAFAG No. 89U, NAFAG AG, Gossau, SG (Switzerland)
- Water (e.g. ad libitum): tap water

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22±3
- Humidity (%): 55±15
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

arachis oil

Doses:

0, 500 and 1000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: mortality and signs were observed daily. Body weight on days 1, 7, 14 and at death.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Statistics:

The LD50 including the 95% confidence limit were computed by the logit method (J. Berkson, J.Am. Stat. Ass. 39. 357-65, 1944).

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 1 000 mg/kg bw

Mortality:

no mortalities were observed.

Clinical signs:

Control (0 mg/kg bw): dyspnea, ruffled fur and curved body position till 5 days post exposure.
500 and 1000 mg/kg bw: sedation, dyspnea, exophthalmus, ruffled fur and curved body position. All symptoms were reversible with 11 days post exposure.

Body weight:

animals gained normal body weight.

Gross pathology:

No compound specific organ changes were observed.