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EC number: 202-708-7 | CAS number: 98-86-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Study with test protocol similar to OECD guideline 402 with acceptable deviations: less detailed documentation of testing procedure, no microscopic examination, confidence interval not specified; sufficient documentation of test results; study acceptable for assessment
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- (less detailed documentation of testing procedure, no microscopic examination, confidence interval not specified)
- Principles of method if other than guideline:
- 5 doses of acetophenone covering a dose range of 1.82-5.2 g/kg were dermally applied with occclusion to groups of 5 male and 5 female rats. Mortality, clinical symptoms and weight development were observed for up to 15 days. All animals found dead and survivors sacrificed on day 15 p.a. were subjected to an autopsy with macroscopic examination of the principal viscera in abdomen and thorax.
- GLP compliance:
- no
- Test type:
- standard acute method
Test material
- Reference substance name:
- Acetophenone
- EC Number:
- 202-708-7
- EC Name:
- Acetophenone
- Cas Number:
- 98-86-2
- Molecular formula:
- C8H8O
- IUPAC Name:
- 1-phenylethanone
- Details on test material:
- - Name of test material (as cited in study report): 39280 R.P., pure acetophenone
- Analytical purity: 99.7%
- Batch No. 77258
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: IFFA CREDO
- Weight at study initiation: 130-150 g
- Fasting period before study: no data
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: 6 x 6 cm
- % coverage: not specified
- Type of wrap if used: aluminium foil and sparadrap
REMOVAL OF TEST SUBSTANCE
- Washing (if done): with soap water
- Time after start of exposure: 24 hrs - Duration of exposure:
- 24 hrs
- Doses:
- 1,82, 2,36, 3, 4, 5,2 g/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days post application
- Frequency of observations and weighing: clinical signs daily, body weight on day 0, 5, 10 and 15
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, macroscopic examination of viscera in abdomen and thorax - Statistics:
- Statistically significant difference of body weight development with p<0.05, p<0.01 (statistical method not specified)
LD50 calculated by method of Dragstedt and Lang
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 300 mg/kg bw
- Based on:
- test mat.
- Mortality:
- 3 g/kg: 3/10; 4 and 5.2 g/kg: all rats died (for details see Table 1)
- Clinical signs:
- other: see Table 1
- Gross pathology:
- Jejunum and ileum identified as target after dermal application
Any other information on results incl. tables
Table 1: Findings after acute dermal application of acetophenone to rats
Dose group (g/kg) |
Mortality |
Clinical symptoms |
Autopsy |
Body weight (g) |
|||||
Incidence |
Time to death p.a. |
Timepoint p.a. |
Symptoms |
Day 0 |
Day 5 |
Day 10 |
Day 15 |
||
0 |
0/10 |
- |
140.5 ±5 |
167.2±8.7 |
184.2±17 |
221±22.9 |
|||
1.82 |
0/10 |
1-2 hrs in 5/10, 1-24 hrs in 5/10 From 6 hrs |
Reduced spontaneous activity Prostration (1/10) and palpebral ptosis (10/10) |
No macroscopic findings |
|||||
2.36 |
0/10 |
Up to 48 hrs Up to 72 hrs |
Reduced spontaneous activity with staggering gait, lacrimation palpebral ptosis (10/10) |
9/10: congestion of mucous layer of jejunum; yellowish liquid in a part of the jejunum |
137.3±4.2 |
155.5±8.4* |
175±15 |
212.5±22.4 |
|
3 |
3/10 |
6 hrs – 6 d |
Up to 5 d 2 hrs – 2 d 6 hrs – 5 d |
Reduced spontaneous activity staggering gait, prostration, tremor; lacrimation, palpebral ptosis Flat and halting breath 1/10 loss of weight and cyanosis on day 5, death on day 6 |
6/7 survivors: mucous intestinal congestion, 2/7 thinning of the abdominal lining in the region of jejunum and ileum, yellowish liquid present |
||||
4 |
10/10 |
6-48 hrs |
From start of exposure |
Staggering gait, prostration and inhibition of turn-around reflex, piloerection, lacrimation, palpebral ptosis, flat breath |
No macroscopic findings |
||||
5.2 |
10/10 |
2-24 hrs |
0 min up to death |
Same as 4 g/kg |
No macroscopic findings |
Applicant's summary and conclusion
- Interpretation of results:
- Category 5 based on GHS criteria
- Conclusions:
- With a LD50 of 3.300 mg/kg in rats, acetophenone showed only very slight toxicity after dermal exposure. Clinical symptoms were typical of effects on the nervous symptoms and on the eyes with a LOAEL of 1820 mg/kg. Autopsy revealed the intestinal tract (jejunum and ileum) to be a target of the adverse action of acetophenone with a LOAEL of 2360 mg/kg.
- Executive summary:
5 doses of acetophenone ranging from 1.82-5.2 g/kg were applied dermally with occclusion to groups of 5 male and 5 female rats. Mortality, clinical symptoms and weight development were observed for up to 15 days. All animals found dead and survivors sacrificed on day 15 p.a. were subjected to an autopsy with macroscopic examination of the principal viscera in abdomen and thorax. With a LD50 of 3.300 mg/kg in rats, acetophenone showed only very slight toxicity after dermal exposure. Clinical symptoms were typical of effects on the nervous symptoms and on the eyes with a LOAEL of 1820 mg/kg. Autopsy revealed the intestinal tract (jejunum and ileum) to be a target of the adverse action of acetophenone with a LOAEL of 2360 mg/kg.
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