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EC number: 201-126-0 | CAS number: 78-59-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: gene mutation
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- study conducted under the auspices of the National Toxicology Program (1983)
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- publication
- Title:
- Chemical mutagenesis testing in Drosophila. X. results of 70 coded chemicals tested for the National Toxicology Program
- Author:
- Foureman P, Mason JM, Valencia R and Zimmering S
- Year:
- 1 994
- Bibliographic source:
- Environ. Molec. Mutagen. 23, 208-227
Materials and methods
- Principles of method if other than guideline:
- Method: NTP SLRL test method
- GLP compliance:
- not specified
- Type of assay:
- Drosophila SLRL assay
Test material
- Reference substance name:
- 3,5,5-trimethylcyclohex-2-enone
- EC Number:
- 201-126-0
- EC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Cas Number:
- 78-59-1
- Molecular formula:
- C9H14O
- IUPAC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Reference substance name:
- Isophorone
- IUPAC Name:
- Isophorone
- Details on test material:
- - origin: Leidy
- lot/batch 1204/01
- analytical purity: 97.2 %
Constituent 1
Constituent 2
Test animals
- Species:
- Drosophila melanogaster
- Strain:
- other: Canton S and Basc stocks
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ORGANISMS:
- Age: adult
- Origin: Canton S and Basc stocks maintained at Brown University and the University of Wisconsin
Administration / exposure
- Route of administration:
- oral: feed
- Vehicle:
- Solvent: ethanol
- Details on exposure:
- ADMINISTRATION:
- Vehicle: ethanol, CAS RN 64-17-5
- Solutions: two or three glass fiber discs were saturated with the test compound carried in solvent (ethanol) at the bottom of a standard glass vial.
Solutions were renewed at 24 hr and 48 hr.
- Duration of test: first mating after 72 hours of exposure
- Frequency of treatment:
- Sampling times and number of samples: three broods, for each >= ca. 5000 chromosomes scored unless mutant frequency > 1.0 %
- Control groups and treatment: concurrent solvent (ethanol)
FOLLOW-UP TESTING:
2-3-day-old males were injected with 0.7 % NaCl solution containing the test chemical at 12,500 ppm. At 24 hours postinjection, toxicity was tested
and survivors were mated. - Duration of treatment / exposure:
- 72 hours
- Frequency of treatment:
- 1 time
- Post exposure period:
- no data
Doses / concentrations
- Remarks:
- Doses / Concentrations:
2000 mg/kg
Basis:
nominal in diet
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- none
Examinations
- Tissues and cell types examined:
- postmeiotic and meiotic germ cells
- Details of tissue and slide preparation:
- For the SLRL test, each male was mated individually to three Basc virgin females, then transferred to fresh Basc virgin females every 2 to 3 days to
make a total of three broods. To reduce the probability of recovering multiple lethals from one male, no more than 100 F1 females were mated over
the three broods from any P1 male. F2 cultures were scored as presumptive lethals if the number of wild-type males was 0, 1, or < 5% of the number
of Basc males. All putative lethals were confirmed through an additional generation. - Evaluation criteria:
- Criteria for evaluating results:
mutation frequency > 0.15 % (P < 0.05): positive
mutation frequency > 0.10 % (P < 0.01): positive
mutation frequency 0.10-0.15 % (P 0.01-0.05): equivocal
other: negative - Statistics:
- For the SLRL assay, a minimum of 5000 chromosomes was scored from each of the treated and concurrent control groups unless the mutant
frequency exceeded 1%.
The treated and control data were compared using a normal approximation to the binomial distribution. In addition, the trated data were compared to the historial control.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Remarks:
- no mutagenic effects were observed
- Toxicity:
- no effects
- Remarks:
- no toxic effects were observed
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Additional information on results:
- MORTALITY:
- feeding: 15 %
- injection: 47 %
PERCENT STERILITY:
- feeding, injection: 0 %
PERCENT LETHALS:
- feeding: 0.11 %; control: 0.18 %
- injection: 0.22 %; control: 0.17 %
RESULT: - feeding, injection: negative
Any other information on results incl. tables
no remarks
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
In this Sex Linked Recessive Lethal (SLRL) test with Drosophila melanogaster no indication of a mutagenic effect was observed. - Executive summary:
In this Sex Recessive Lethal (SLRL) test with Drosophila melanogaster, fruit flies were fed a diet containing 2000 mg/kg isophorone. After 72 hrs of exposure, surviving males were mated. As feeding exposure was found to be non-mutagenic, 2 -3 day old males were injected with a 0.7 % NaCl solution containing 12,500 mg/l isophorone. 24 hrs post injection, males were mated. Again there was no indication of a mutagenic effect. Concurrent control males were treated with ethanol used to dissolve the test chemical.
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