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EC number: 204-706-1 | CAS number: 124-63-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.6 (Skin Sensitisation)
- Version / remarks:
- Cited as Directive 96/54/EC, B.6
- GLP compliance:
- yes
- Type of study:
- guinea pig maximisation test
Test material
- Reference substance name:
- Methanesulphonyl chloride
- EC Number:
- 204-706-1
- EC Name:
- Methanesulphonyl chloride
- Cas Number:
- 124-63-0
- Molecular formula:
- CH3ClO2S
- IUPAC Name:
- methanesulfonyl chloride
- Details on test material:
- Origin: Atofina, Riverview
plant
Batch: 1491F10CD1
Dry extract: 99.9 %
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- male/female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Charles River Laboratories France, L’Arbresle, France.
- Age at study initiation: 1-2 months old
- Weight at study initiation: 458 ± 19 g for the males and 383 ± 16 g for the females
- Housing: individually in polycarbonate cages with stainless steel lid
- Diet (e.g. ad libitum): 1096 pelleted diet (SAFE, Villemoisson, Epinay-sur-Orge, France)
- Water (e.g. ad libitum): drinking water filtered by a FG Millipore membrane (0.22 micron)
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2
- Humidity (%): 30 to 70
- Air changes (per hr): approximately 12 cycles/hour of filtered, non-recycled air.
- Photoperiod (hrs dark / hrs light): 12/12
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal and epicutaneous
- Vehicle:
- paraffin oil
- Concentration / amount:
- Intradermal: 2 mg/l
Epicutaneous: 200 mg/ml
Challenge: 200, 50 or 20 mg/ml
Challengeopen allclose all
- Route:
- epicutaneous, occlusive
- Vehicle:
- paraffin oil
- Concentration / amount:
- Intradermal: 2 mg/l
Epicutaneous: 200 mg/ml
Challenge: 200, 50 or 20 mg/ml
- No. of animals per dose:
- 20, test group, 10 control group
- Details on study design:
- Methanesulfonyl chloride was provided as a 200.25 mg/mL solution in paraffin oil for ease of manipulation.
Thirty guinea pigs were allocated to two groups: a control group of five males and five females and a treated group of ten males and ten females.
On day 1, three pairs of intradermal injections were performed in the interscapular region of all animals:
* Freund's complete adjuvant (FCA) diluted to 50% with 0.9% NaCl (both groups),
* test item at the concentration of 2 mg/mL (i.e. solution at 1%) in paraffin oil (treated group) or vehicle alone (control group),
* test item at the concentration of 2 mg/mL (i.e. solution at 1%) in a mixture FCA/0.9% NaCl (50/50) (treated group) or vehicle at the concentration of 50% in a mixture FCA/0.9% NaCl (50/50) (control group).
On day 8, the animals of the treated group received a topical application of the test item at the concentration of 200 mg/mL in paraffin oil (i.e. undiluted solution) to the same test site, which was then covered by an occlusive dressing for 48 hours. The animals of the control group
received an application of vehicle under the same experimental conditions.
On day 22, all animals of both groups were challenged by a cutaneous application of the test item at the concentration of 200 mg/mL in paraffin oil (i.e. undiluted solution) to the right flank. The test item was maintained under an occlusive dressing for 24 hours. The vehicle was applied to the left flank under the same experimental conditions.
Skin reactions were evaluated approximately 24 and 48 hours after removal of the dressing.
As equivocal cutaneous reactions were noted after the first challenge, a second challenge application was performed on day 40. For this second challenge application, a control group of ten naive animals (five males and five females) was included in the study. The test item in paraffin oil was applied at the concentration of 50 mg/mL (i.e. solution at 25% (w/w)) to the left flank and at the concentration of 20 mg/mL (i.e. solution at 10% (w/w)) to the right flank of
animals of all groups, under the same experimental conditions as for the first challenge application.
At the end of the study, animals were killed without examination of internal organs.
No skin samples were taken from the challenge application sites. - Positive control substance(s):
- yes
- Remarks:
- Mercaptobenzothiazole
Results and discussion
- Positive control results:
- Mercaptobenzothiazole at the concentration of 20% (w/w) induced positive skin sensitization reactions in 80% (8/10) guinea pigs.
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 200 mg/ml
- No. with + reactions:
- 8
- Total no. in group:
- 10
- Clinical observations:
- discrete or patchy erythema (grade 1)
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: negative control. Dose level: 200 mg/ml. No with. + reactions: 8.0. Total no. in groups: 10.0. Clinical observations: discrete or patchy erythema (grade 1).
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 200 mg/ml
- No. with + reactions:
- 20
- Total no. in group:
- 20
- Clinical observations:
- moderate or intense erythema (grade 2 or 3)
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 200 mg/ml. No with. + reactions: 20.0. Total no. in groups: 20.0. Clinical observations: moderate or intense erythema (grade 2 or 3).
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 200 mg/ml
- No. with + reactions:
- 9
- Total no. in group:
- 10
- Clinical observations:
- discrete or moderate erythema (grade 1 or 2)
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: negative control. Dose level: 200 mg/ml. No with. + reactions: 9.0. Total no. in groups: 10.0. Clinical observations: discrete or moderate erythema (grade 1 or 2).
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 200 mg/ml
- No. with + reactions:
- 20
- Total no. in group:
- 20
- Clinical observations:
- moderate or intense erythema (grade 2 or 3)
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: test group. Dose level: 200 mg/ml. No with. + reactions: 20.0. Total no. in groups: 20.0. Clinical observations: moderate or intense erythema (grade 2 or 3).
- Reading:
- rechallenge
- Hours after challenge:
- 24
- Group:
- other: naive control group
- Dose level:
- 50 mg/ml
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 24.0. Group: other: naive control group. Dose level: 50 mg/ml. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- rechallenge
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 50 mg/ml
- No. with + reactions:
- 2
- Total no. in group:
- 10
- Clinical observations:
- discrete erythema (grade 1)
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 24.0. Group: negative control. Dose level: 50 mg/ml. No with. + reactions: 2.0. Total no. in groups: 10.0. Clinical observations: discrete erythema (grade 1).
- Reading:
- rechallenge
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 50 mg/ml
- No. with + reactions:
- 13
- Total no. in group:
- 18
- Clinical observations:
- discrete or moderate erythema (grade 1 or 2)
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 24.0. Group: test group. Dose level: 50 mg/ml. No with. + reactions: 13.0. Total no. in groups: 18.0. Clinical observations: discrete or moderate erythema (grade 1 or 2).
- Reading:
- rechallenge
- Hours after challenge:
- 48
- Group:
- other: naive control group
- Dose level:
- 50 mg/ml
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 48.0. Group: other: naive control group. Dose level: 50 mg/ml. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- rechallenge
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 50 mg/ml
- No. with + reactions:
- 2
- Total no. in group:
- 10
- Clinical observations:
- discrete erythema (grade 1)
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 48.0. Group: negative control. Dose level: 50 mg/ml. No with. + reactions: 2.0. Total no. in groups: 10.0. Clinical observations: discrete erythema (grade 1).
- Reading:
- rechallenge
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 50 mg/ml
- No. with + reactions:
- 11
- Total no. in group:
- 18
- Clinical observations:
- discrete or moderate erythema (grade 1 or 2)
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 48.0. Group: test group. Dose level: 50 mg/ml. No with. + reactions: 11.0. Total no. in groups: 18.0. Clinical observations: discrete or moderate erythema (grade 1 or 2).
- Reading:
- rechallenge
- Hours after challenge:
- 24
- Group:
- other: naive control group
- Dose level:
- 20 mg/ml
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 24.0. Group: other: naive control group. Dose level: 20 mg/ml. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- rechallenge
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- 20 mg/ml
- No. with + reactions:
- 9
- Total no. in group:
- 10
- Clinical observations:
- discrete or moderate erythema (grade 1 or 2)
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 24.0. Group: negative control. Dose level: 20 mg/ml. No with. + reactions: 9.0. Total no. in groups: 10.0. Clinical observations: discrete or moderate erythema (grade 1 or 2).
- Reading:
- rechallenge
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 20 mg/ml
- No. with + reactions:
- 18
- Total no. in group:
- 18
- Clinical observations:
- discrete to intense erythema (grades 1 to 3)
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 48.0. Group: test group. Dose level: 20 mg/ml. No with. + reactions: 18.0. Total no. in groups: 18.0. Clinical observations: discrete to intense erythema (grades 1 to 3).
- Reading:
- rechallenge
- Hours after challenge:
- 48
- Group:
- other: naive control group
- Dose level:
- 20 mg/ml
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 48.0. Group: other: naive control group. Dose level: 20 mg/ml. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- rechallenge
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- 20 mg/ml
- No. with + reactions:
- 9
- Total no. in group:
- 10
- Clinical observations:
- discrete or moderate erythema (grade 1 or 2)
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 48.0. Group: negative control. Dose level: 20 mg/ml. No with. + reactions: 9.0. Total no. in groups: 10.0. Clinical observations: discrete or moderate erythema (grade 1 or 2).
- Reading:
- rechallenge
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 20 mg/ml
- No. with + reactions:
- 18
- Total no. in group:
- 18
- Clinical observations:
- discrete to intense erythema (grades 1 to 3) with crust
- Remarks on result:
- other: Reading: rechallenge. . Hours after challenge: 48.0. Group: test group. Dose level: 20 mg/ml. No with. + reactions: 18.0. Total no. in groups: 18.0. Clinical observations: discrete to intense erythema (grades 1 to 3) with crust.
Any other information on results incl. tables
No
systemic clinical signs and no deaths related to treatment were noted
during the study.
After the first challenge application, a discrete or moderate erythema
was observed in 9/10 animals of the control group.
In the treated group, a moderate or intense erythema was noted in all
animals. Dryness of the skin, crusts (which have sometimes masked the
evaluation of erythema) and oedema were also recorded.
After the second challenge application, no cutaneous reactions were
observed in the animals of the control group 3.
In the control group 1, a discrete erythema was observed on the left
flank of 2/10 animals and a discrete or moderate erythema was noted on
the right flank of 9/10 animals at the 24 and 48-hour readings.
In the treated group, a discrete or moderate erythema was recorded on the
These results confirmed those of the first challenge application. The cutaneous reactions observed in all the animals of the treated group after the first challenge application, which were of higher severity than those recorded in the animals of the control group, were therefore attributed to delayed contact hypersensitivity.
Applicant's summary and conclusion
- Interpretation of results:
- sensitising
- Remarks:
- Migrated information
- Conclusions:
- According to the maximization method of Magnusson and Kligman, Methanesulfonyl chloride induces delayed contact hypersensitivity in 20/20 (100%) guinea pigs.
Classification: sensitizing - Executive summary:
In a test performed according to the maximization method of Magnusson and Kligman, the OECD guideline #406 and the GLP, methanesulfonyl chloride (purity 99.9%) was administered to guinea pigs at the concentrations of 2 mg/ml intracutaneously and 200 mg/ml in paraffin oil under an occlusive patch.
The challenge concentration of 200 mg/ml under an occlusive patch induced delayed contact hypersensitivity in 20/20 (100%) guinea-pigs.Methanesulfonyl chloride was considered as a strong skin sensitizer.
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