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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 2005-09-15 to 2005-10-14
Reliability:
1 (reliable without restriction)
Cross-reference
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Version / remarks:
adopted 1992-07-17
Deviations:
no
Principles of method if other than guideline:
NA
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Details on test material:
Batch no: EZ06-B001;
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
NA

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, adapted
Details on inoculum:
The activated sludge was washed twice with autoclaved tab water and maintained in an aerobic condition by aeration for 4 hours and then homogenised with a mixer. Thereafter the sludge was filtered with folded filter and aerated with CO2-free air for 5 days. 25 ml/l were used to initiate inoculation.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
15 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
NA
Reference substance
Reference substance:
acetic acid, sodium salt

Results and discussion

Preliminary study:
NA
Test performance:
The functional and the toxicity control confirmed the validity of the test.
% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
17
Sampling time:
28 d
Details on results:
NA

BOD5 / COD results

Results with reference substance:
34 % biodegradation occurred within 14 days and with a maximum of 43 % after 28 days.

Any other information on results incl. tables

NA

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: not ready biodegradable
Conclusions:
The test item is not ready biodegradable.
Executive summary:

The test item is not ready biodegradable.