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EC number: 248-273-7 | CAS number: 27157-94-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- O,O-bis(methylphenyl) hydrogen dithiophosphate
- EC Number:
- 248-273-7
- EC Name:
- O,O-bis(methylphenyl) hydrogen dithiophosphate
- Cas Number:
- 27157-94-4
- Molecular formula:
- C14H15O2PS2
- IUPAC Name:
- O,O-bis(2-methylphenyl) sulfanylphosphonothioate
- Reference substance name:
- Phosphorodithioic acid, O,O-bis(methylphenyl) ester
- IUPAC Name:
- Phosphorodithioic acid, O,O-bis(methylphenyl) ester
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): Cresyl-P1
- Physical state: Liquid
- Stability under test conditions: Stable
- Storage condition of test material: ambient
Constituent 1
Constituent 2
Method
- Target gene:
- Strain Designation Histidine Gene Locus Affected Additional Mutations Type of Mutation Detected
Repair LPS Plasmids
TA 98#1 his D 3052 uvr B- rfa- pKM 101 Frameshift
TA 100#1 his G 46 uvr B- rfa- pKM 101 Base-pair substitution
TA 102#1 his G 428 wild-type rfa- pKM 101 / pAQ1 Base-pair substitution
TA 1535#2 his G 46 uvr B- rfa- none Base-pair substitution
TA 1537#2 his C 3076 uvr B- rfa- none Frameshift
rfa-: partial loss of lipopolysaccharide (LPS) barrier that causes increased permeability to macromolecules
uvr B-: loss of DNA excision repair system
pKM 101: R-factor plasmid, thought to cause an increased error-prone DNA repair
pAQ1: plasmid, carrier of tetracycline resistance
#1 resistance to Ampicillin
#2 non-resistance to Ampicillin
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- a microsomal preparation derived from Aroclor 1254-induced rat liver.
- Test concentrations with justification for top dose:
- 5.0, 15.8, 50, 158, 500 and 1580 µg per plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: ethanol (Batch no. B1810; Honeywell Specialty Chemicals Seelze GmbH, 30926 Seelze Germany)
- Justification for choice of solvent/vehicle: Cresyl-P1 (DANAFLOAT™ 070) was not soluble in dimethyl sulfoxid (DMSO), water, methanol or acetone.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Remarks:
- ethanol
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- in aqua ad iniectabilia (10 µg/plate) : TA 1535, TA 100
- Untreated negative controls:
- yes
- Remarks:
- ethanol
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- in DMSO (10 µg/plate) : TA 98
- Untreated negative controls:
- yes
- Remarks:
- ethanol
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- in ethanol, abs. (100 µg/plate) : TA 1537
- Untreated negative controls:
- yes
- Remarks:
- ethanol
- Positive controls:
- yes
- Positive control substance:
- mitomycin C
- Remarks:
- in DMSO (10 µg/plate) : TA 102
- Untreated negative controls:
- yes
- Remarks:
- ethanol
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- in DMSO (10 µg/plate) : TA 98, TA 102, TA 1537
- Untreated negative controls:
- yes
- Remarks:
- ethanol
- Positive controls:
- yes
- Positive control substance:
- other: 2-amino-anthracene
- Remarks:
- in DMSO (2 µg/plate) : TA 100, TA 1535
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: 20 minutes at 37°C
- Exposure duration: 48-72 hours at 37°C - Evaluation criteria:
- A test item is considered to show a positive response if
- the number of revertants is significantly increased (p ≤ 0.05, U-test according to MANN and WHITNEY, see COLQUHOUN, D.: Lectures on Biostatistics, Clarendon Press, Oxford (1971)) compared with the solvent control to at least 2-fold of the solvent control for TA 98, TA 100 and TA 102 and 3-fold of the solvent control for TA 1535 and TA 1537 in both independent experiments;
- in addition, a significant (p ≤ 0.05) concentration (log value)-related effect (Spearman’s rank correlation coefficient, see COLQUHOUN, D.: Lectures on Biostatistics, Clarendon Press, Oxford (1971)) is observed;
- positive results have to be reproducible and the histidine independence of the revertants has to be confirmed by streaking random samples on histidine-free agar plates.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- No increase in revertant colony numbers as compared with control counts was observed for test item, tested up to a cytotoxic concentration of 3250 µg/plate, in any of the 5 test strains in two independent experiments w./wo metabolic activation, respective
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- In the plate incorporation test and the preincubationcubation test, each carried out without and with metabolic activation cytotoxicity (scarce background lawn and reduction of the number of revertants) was noted at the top concentration of 1580 µg /plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- It can be concluded that the test material is negative with and without metabolic activation in all tester strains.
Under the present test conditions Cresyl-P1 tested up to a cytotoxic concentration of 1580 µg Cresyl-dtp/plate, caused no mutagenic effect in the Salmonella typhimurium strains TA 98, TA 100, TA 102, TA 1535 and TA 1537 neither in the plate incorporation test nor in the preincubation test each carried out without and with metabolic activation. - Executive summary:
The registered substance was tested for its mutagenicity in an Ames Plate Incorporation Assay according to OECD Guideline 471 and under GLP in Salmonella typhimurium strains TA 98, TA 100, TA 102, TA 1535 and TA 1537 with and without metabolic activation.
Under the present test conditions Cresyl-P1 tested up to a cytotoxic concentration of 1580 µg Cresyl-dtp/plate, caused no mutagenic effect in the Salmonella typhimurium strains TA 98, TA 100, TA 102, TA 1535 and TA 1537 neither in the plate incorporation test nor in the preincubation test each carried out without and with metabolic activation.
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