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EC number: 700-831-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 February 2011 to 10 June 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was conducted in accordance with International Guidelines and in accordance with the OECD Principles of Good Laboratory Practice as incorporated into the United Kingdom (Statutory Instrument for GLP).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,2,2,4,4,4,4-heptakis[(2-ethylhexanoyl)oxy]cyclodimolybdoxan-2-yl 2-ethylhexanoate
- EC Number:
- 700-831-5
- Molecular formula:
- Mo2O2L8 (L = 2-ethylhexanoic acid; C8H16O2) (L' = 2-ethylhexanoic acid; C8H16O2)
- IUPAC Name:
- 2,2,2,4,4,4,4-heptakis[(2-ethylhexanoyl)oxy]cyclodimolybdoxan-2-yl 2-ethylhexanoate
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 Mix
- Test concentrations with justification for top dose:
- In the absence of S9 mix, the concentrations tested were: 5, 17, 50, 167, 500 and 1667 μg per plate
In the presence of S9 mix, the concentrations tested were: 17, 50, 167, 500, 1667 and 5000 μg per plate - Vehicle / solvent:
- Ethanol was used as the vehicle control and was plated in triplicate (50 μL per plate) with each strain used, both in the absence and in the presence of S9 mix.
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: With S9 mix: 2-Aminoanthracene (2AAN): 2 μg per plate with S typhimurium TA 1535 and TA 1537, 0.5 μg per plate with S. typhimurium TA 98 and TA 100 and 20 μg per plate with E. coli WP2uvrA
- Positive control substance:
- other: Without S9 mix: Sodium azide (NaN3): 1 μg per plate with S. typhimurium TA 1535 and TA 100 9-Aminoacridine (9-AA): 80 μg per plate with S. typhimurium TA 1537
- Positive control substance:
- other: Without S9 Mix: 2-Nitrofluorene (2-NF): 1 μg per plate with S. typhimurium TA 98 N-Ethyl-N-nitro-N-nitrosoguanidine (ENNG): 2 μg per plate with E. coli WP2uvrA
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- only in the absence of S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- Toxicity Tests
In the absence of S9 mix, concentrations of 500 μg per plate and above were toxic to the bacteria. Toxicity was observed as a reduction in the number of revertant colonies and as a slight thinning of the background lawn of microcolonies. No precipitation of the test item occurred.
In the presence of S9 mix, no toxicity was observed. HCAT precipitated at the highest concentration of 5000 μg per plate.
Evidence of mutagenic activity was not obtained with HCAT in any strain in either test.
In the first mutation assay, conducted by the direct plate incorporation method, toxicity to the bacteria was observed in the absence of S9 mix as a slight thinning of the background lawn of microcolonies at the 2 highest concentrations of 500 and 1667 μg per plate. In the presence of S9 mix, toxicity to the bacteria was observed only at 5000 μg per plate in strain TA 1537. HCAT precipitated at 5000 μg per plate in the presence of S9 mix.
In the second mutation assay, conducted by the pre-incubation method, toxicity to the bacteria was observed in the absence of S9 mix at 1667 μg per plate in all strains and at 500 μg per plate in strains TA1535 and TA 1537. In the presence of S9 mix, toxicity to the bacteria was observed at 5000 μg per plate in all S. typhimurium strains. HCAT precipitated at 5000 μg per plate in the presence of S9 mix. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
In conclusion, HCAT was not mutagenic in strains of S. typhimurium and E. coli when dissolved and diluted in ethanol and tested in the absence and presence of metabolic activation. HCAT was tested at concentrations that extended into the toxic range in the absence of metabolic activation and up to the predetermined maximum concentration of 5000 μg per plate in the presence of metabolic activation. This latter treatment was beyond the limit of HCAT’s solubility in the test system. - Executive summary:
HCAT was tested for mutagenic activity in Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 and in Escherichia coli WP2uvrA.
Two independent tests were conducted on agar plates in triplicate in the absence and presence of an Aroclor 1254-induced rat liver S9 preparation and the co-factors required for mixed- function oxidase activity (S9 mix). The first test was conducted by the direct plate incorporation method, while the second test was conducted by the pre-incubation method. HCAT was dosed at concentrations ranging from 5 to 1667 μg per plate in the absence of S9 mix and from 17 to 5000 μg per plate in the presence of S9 mix. HCAT was dissolved and diluted in ethanol.
Concurrent positive controls demonstrated the sensitivity of the assay and the metabolising activity of the S9 mix.
Evidence of mutagenic activity was not obtained with any strain in either test.
Dependent on strain and test conditions, HCAT was toxic to the bacteria at the higher concentrations. This toxicity was observed either as a reduction in the number of revertant colonies, or as a thinning of the background lawn of microcolonies. Precipitation of HCAT occurred in the presence of S9 mix only, at the highest concentration of 5000 μg per plate.
In conclusion, HCAT was not mutagenic in strains of S. typhimurium and E. coli when dissolved and diluted in ethanol and tested in the absence and presence of metabolic activation. HCAT was tested at concentrations that extended into the toxic range in the absence of metabolic activation and up to the predetermined maximum concentration of 5000 μg per plate in the presence of metabolic activation. This latter treatment was beyond the limit of HCAT’s solubility in the test system.
The study was performed in accordance with the principles of Good Laboratory Practice.
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