Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 220-395-5 | CAS number: 2752-17-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Two instead of five animals per sex per dose are used. At half of the test animals, the skin is abraded before application of the test substance.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- Two instead of five animals per sex per dose are used. At half of the test animals, the skin is abraded before application of the test substance.
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2,2'-oxydi(ethylamine)
- EC Number:
- 220-395-5
- EC Name:
- 2,2'-oxydi(ethylamine)
- Cas Number:
- 2752-17-2
- Molecular formula:
- C4H12N2O
- IUPAC Name:
- 2-(2-aminoethoxy)ethan-1-amine
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 5601-22-1, Order #J-169
- Substance type: clear liquid
- Physical state: liquid
- Base factor: 1.047 gm/ml
- Purity: responsibility of the sponsor
- Other: stability: there was no apparent change in the physical state of the test article during administration
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Perfection Breeders, Douglasseville, Pennsylvania and Sgarlat's Rabbitry, Harvey's Lake, Pennsylvania
- Weight at study initiation: 2 to 3 kilograms
- Housing: Separate isolation by test system
- Diet (e.g. ad libitum): Wayne Rabbit Ration, ad libitum, checked daily and added or replaced as needed. Feeders are designed to reduce soiling, bridging and scattering.
- Water (e.g. ad libitum): Availability - fresh tap water, fit for human consumption, ad libitum using 16 ounce glass bottles with rubber stopper and stainless steel sipper tube or an automatic watering system supplied by Edstrom Industries, Inc., Waterford, Wisconsin.
- Acclimation period: five days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20°C ± 3°C
- Humidity (%): 30 - 70%
- Photoperiod (hrs dark / hrs light): light cycle - 12 hours light, 12 hours dark
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: shaved trunk
- % coverage: no less than 20% of the dorsal body surface area
- Type of wrap if used: The test article was applied directly onto the exposed skin of the animals taking care to spread the substance evenly over the entire abraded area. A layer of gauze was wrapped around the animals to cover the dosed area. The animals were wrapped with rubber dam and an ace bandage to retard evaporation. The test article was held in contact with the skin for twenty-four hours.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Following the twenty-four hour period of exposure, the rubber dam and ace bandage were removed. The test site was washed to remove any remaining material.
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1 quart - Duration of exposure:
- 24 hours
- Doses:
- 2500, 3200, 4000, 5000, 6300 and 8000 mg/kg bw
- No. of animals per sex per dose:
- 2
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days: Observations were recorded at 30 minutes, 2 and 4 hours after the 24 hour period of exposure, and twice daily thereafter for fourteen days. All rabbits were sacrificed by CO2 inhalation on Day 14 and a gross necropsy was performed.
- Statistics:
- By the method of Litchfield and Wilcoxon (1949).
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 3 550 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 2 530 - 4 980
- Mortality:
- Dose-range finding study: None of the rabbits died at the 1000 mg/kg dose level. One of two rabbits died at the 3000, 5000 and 8000 mg/kg dose levels.
Dermal LD50 determination:
None of the rabbits died at 2500 mg/kg, two of four rabbits died at 3200 mg/kg, four of four died at 4000 mg/kg, two of four at 5000 mg/kg and four of four died at the 6300 and 8000 mg/kg dose levels. - Clinical signs:
- other: Dose-range finding study: Signs included necrosis, slight edema, ataxia, decreased activity, bleeding from the anal and genital areas, body drop, diarrhea, abnormal gait, abnormal stance and decreased body tone. Dermal LD50 determination: Signs observed
- Gross pathology:
- Necropsy of the animals dying on the study revealed dark discolorations and hemorrhages at the application sites, pale kidneys, dark red fluid-filled bladders and pale livers with multiple small irregular yellow nodules, hemorrhages in the cortex of both kidneys, fluid-filled intestines and erosions in the stomach mucosa. Fluid in the thoracic cavity, discolored adrenals, distended bladders, congested lungs and distended fluid-filled intestines were also observed. Terminal necropsy revealed hemorrhages of the muscle layers under the application sites, fluid in the abdominal cavity, liver adhered to abdominal walls, kidneys pitted with surface hemorrhages and small areas of necrosis on the skin sites.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Based upon the observations made in the Acute Dermal Toxicity Study in Rabbits, the calculated dermal LD50 for the test substance was determined to be 3550 mg/kg with 95% confidence limits of 2530 to 4980 mg/kg. Based on the test results and according to the criteria of the CLP Regulation, the substance should not be classified for acute dermal toxicity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.