Aluminium, 6-hydroxy-5-[(4-sulfophenyl)azo]-2-naphthalenesulfonic acid complex

Long-term toxicity to aquatic invertebrates

Description of key information

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on daphnia magna was predicted for aluminium, 6-hydroxy-5-[(4-sulfophenyl)azo]- 2-naphthalenesulfonic acid complex (15790-07-5). The EC50 value was estimated to be 83.527 mg/l when aluminium, 6-hydroxy-5-[(4-sulfophenyl)azo]-2-naphthalenesulfonic acid complex exposed to daphnia magna for 504 hrs. Thus based on the EC50 it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

83.527 mg/L

Additional information

Based on the various predicted data for the target chemical and experimental data for read across chemicals study have been reviewed to determine the toxic nature of target chemical aluminium, 6-hydroxy-5-[(4-sulfophenyl)azo]-2-naphthalenesulfonic acid complex (15790-07-5). The studies are as mentioned below: 

 

In the first weight of evidence study for the target chemical (15790-07-5) toxicity was predicted based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on daphnia magna was predicted for aluminium, 6-hydroxy-5-[(4-sulfophenyl)azo]- 2-naphthalenesulfonic acid complex (15790-07-5). The EC50 value was estimated to be 83.527 mg/l when aluminium, 6-hydroxy-5-[(4-sulfophenyl)azo]-2-naphthalenesulfonic acid complex exposed to daphnia magna for 504 hrs. Thus based on the EC50 it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

 

In the second weight of evidence study for the read across chemical (657-84-1) from J-check study on determination of the inhibition of the mobility of daphnids was carried out with the substance Aluminium,6-hydroxy-5- [(2-methoxy-5-methyl-4-sul fophenyl)azo ]-2-naphthalenesulfonic acid complex according to OECD Guideline 211. Acute Immobilization Test of 4-methylbenzenesulfonic acid (Sodium p-toluenesulfonate) to Daphnia magna was studied for 21 days. Test was performed according to the OECD Guideline 211 (Daphnia magna Reproduction Test). Test performed in the semi-static system. Control, 100, 32, 10 mg/L nominal concentration was added in the test vessel filled with 80 ml of test solution. Test conducted in the ten replicates, 10 daphnia magna per concentration (1 per vessel was added). Based on the effect of chemical 4-methylbenzenesulfonic acid (Sodium p-toluenesulfonate) on the normal activity of daphnia magna for the 21 days, the EC50 was ≥ 100 mg/l and NOEC was 100 mg/l. Thus based on the EC50, it can be concluded that the 4-methylbenzenesulfonic acid was nontoxic and can be consider to be not classified as per the CLP classification criteria. 

 

Similarly third weight of evidence study supports the first predicted study for RA chemical (5281-04-9) from OECD SIDS report, 1994 similar to the target chemical. Study was conducted to determine the long term toxicity of calcium 3-hydroxy-4-[(4-methyl-2-sulfonatophenyl)diazenyl]-2-naphthoate on the mortality rate of daphnia magna. Test conducted in the open system by semi-static method. 4 replicates were used in which 10 organisms were used in each replicates. Control of DMSO: HCO-40 was 9:1 (100 mg/kg). Test conducted on the nominal concentration for 7 days, 14 days and 21days. After the exposure of chemical lethal concentration was measured. Based on the mortality rate of daphnia magna due to the long period exposure with chemical calcium 3-hydroxy-4-[(4-methyl-2 sulfonatophenyl) diazenyl]-2-naphthoate, the LC50 value was 13 mg/l, 10 mg/l and 9.7 mg/l for 7 days, 14 days and 21 days of exposure. The EC50 was 4.4 and 9.1 after 14 and 21 days of exposure. Based on the LC50, it can be concluded that the chemical was nontoxic and not classified as per the CLP classification criteria.

 

Based on the data from various data, it can be concluded that the substance aluminium, 6-hydroxy-5-[(4-sulfophenyl)azo]-2-naphthalenesulfonic acid complex (15790-07-5) is considered to be not toxic to aquatic environment and cannot be classified as toxic as per the criteria mentioned in CLP regulation.