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EC number: 700-978-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: study was well performed and documented, according to OECD and GLP guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- trisodium 2-(2-sulfonatoethoxy)ethane-1-sulfonate ethenesulfonate
- EC Number:
- 700-978-5
- Molecular formula:
- C2H3O3S.Na C4H8O7S2Na2
- IUPAC Name:
- trisodium 2-(2-sulfonatoethoxy)ethane-1-sulfonate ethenesulfonate
- Details on test material:
- - Batch/Lotnr: 200911040014
- Production date: 04/11/2009
- Shelf-life: 6 months
- Purity of active: 25.09%
- Details of supplier: Proviron Functional chemicals nv
- Date of receipt: 05/02/2010
- Storage: Ambient room temperature 20 to 30°C
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- sewage, industrial
- Details on inoculum:
- - Source: collected from Sri Venkateswara Sugar Mill, Palayasivaram, Kancheepuram District, Tamil Nadu.
- Dry weight was determined and enriched by adding synthetic waste-water (50 ml per litre of sewage sludge)
- Mixture was aerated overnight at 20 +/- 2°C and used for the study
- Sludge containing 3.9 g/L dry weight solid, filterable components
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- pH:
- 7.42-7.82
- Nominal and measured concentrations:
- 2.1; 5.92; 18.56; 60.02; 120.16 g/L + control
- Details on test conditions:
- SEWAGE FEED
- synthetically prepared by dissolving following ingredient in one liter of water: 16.0 g peptone, 11 g meat extract, 3 g urea
0.7 g sodium chloride, 0.4 g calcium chloride, 0.2 g magnesium sulphate and 2.8 g dipotassium hydrogen phosphate.
TEST PROCEDURE
- Preparation of stock solution
- Aeration
- the following were added into 500 ml flat bottom flask and aerated for 3h: 16 ml synthetic sewage feed; X ml stock solution (5; 10; 20; 10 and 80 ml) of 3,5-dichlorophenol/ x mg of Sodium vinyl sulphonate solution (1.0500; 2.9802; 9.4750; 30.0101; 60.0801g); approximately 300 ml water; 200 ml aerated sewage sludge and made up to 500 ml using water
- After the end of 3h, the aeration was stopped. The decrease in oxygen was determined over a period of 10 minutes by reading off the oxygen concentration at every minute.
- The measurements of the oxygen concentrations were carried out with calibrated dissolved oxygen meter. - Reference substance (positive control):
- yes
- Remarks:
- 500 mg of 3,5-dichlorophenol was dissolved in 10 ml of 1N NaOH and diluted to 30 ml with distilled water. Then 8 ml of 1N H2SO4 was added by continuous stirring and finally the volume was made upto 1L with distilled water. The pH stock solution = 7.08
Results and discussion
Effect concentrations
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 120 g/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- 3h EC50: 22.49 mg/L (measured) with 3,5-dichlorophenol
Any other information on results incl. tables
the respiration rate was determined from the linear range of the oxygen decrease. From the rates of oxygen decrease, the inhibition of the respiration rate of the sewage sludge was calculated by using the following formula:
%inhibition = 1 - (2Rs/(Rc1 +Rc2)) x 100
where Rs = oxygen consumption rate at tested concentration of test substance
Rc1 = oxygen consumption rate, control 1
Rc2 = oxygen consumption rate, control 2
the decrease in oxygen was determined over a period of 10 minutes by reading off the oxygen concentration at every minute.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- From the results it was observed that the test item is non-inhibitory to the microbial organisms up to 120 g/L. The EC50 for Sodium vinyl sulphonate solution (Provichem 2202) after an exposure time of 3 hours was > 120 g/L.
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