Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 265-512-0 | CAS number: 65140-91-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
Bacterial mutagenicity
In an Ames assay equivalent to OECD TG 471, the Salmonella tester strains TA1535, TA 1537, TA 1538, TA 98, TA 100 and the E. coli WP2 uvr A were treated with 5, 10, 50, 100, 500, 1000 and 5000 µg test substance per plate (Ciba-Geigy, 1981). The experiments were performed in the presence and absence of a metabolic activation system (S9 fraction of liver from rats induced with Aroclor 1254). Solvent and positive controls were included in this study and have shown to be valid. In contrast to actual regulations (OECD TG 471, adopted 1997) 2-Aminoanthracene was the sole positive control in the presence of metabolic activation. In the experiments performed without and with microsomal activation, comparison of the number of histidine- or tryptophanprototrophic mutants in the controls and after treatment with test substance revealed no marked differences. Thus, the test substance showed no potential to induce gene mutations in bacteria.
Mutagenicity in mammalian cells (HPRT)
A GLP-compliant mammalian cell mutagenicity test according to OECD guideline 476 was performed to investigate the potential of the test article to induce gene mutations at the HPRT locus in V79 cells of the Chinese hamster (BASF, 2012). The assay was performed in two independent experiments, using two parallel cultures each. The first main experiment was performed with and without liver microsomal activation and a treatment period of 4 hours. The second experiment was performed with a treatment time of 4 hours with and 24 hours without metabolic activation. The highest concentration of 1600 μg/mL was limited by the solubility properties of the test item in organic solvents and aqueous medium. The test item was dissolved in DMSO. The tested concentrations ranged from 100 to 1600 µg/ml. Precipitation of the test item visible to the naked eye at the end of treatment was noted at 1200 μg/mL and above in experiment I with metabolic activation and at 800 μg/mL and above in experiment II with metabolic activation. Relevant cytotoxic effects indicated by a relative cloning efficiency I or cell density below 50% in both parallel cultures solely occurred in the first experiment at 1200 μg/mL and above without metabolic activation. No substantial and reproducible dose dependent increase of the mutation frequency was observed up to the maximum concentration with and without metabolic activation. Appropriate reference mutagens (EMS and DMBA), used as positive controls, induced a distinct increase in mutant colonies and thus, showed the sensitivity of the test system and the activity of the metabolic activation system. In conclusion it can be stated that under the experimental conditions reported the test item did not induce gene mutations at the HPRT locus in V79 cells. Therefore, the test article is considered to be non-mutagenic in this HPRT assay.
Chromosome damage (in vivo)
In a Nucleus Anomaly Test, 3 Chinese hamsters per sex were treated with 1250, 2500, and 5000 mg/kg bw test substance by gavage (Ciba-Geigy, 1983). Treatments were performed once daily on 2 consecutive days. Cyclophosphamide was used as a positive control substance, and a vehicle control was used as negative control. 24 h after the second treatment the animals were sacrificed and the bone marrow was prepared for scoring. 1000 bone marrow cells were scored per animal, and the following parameters were investigated: Single Jolly bodies, fragments of nuclei in erythrocytes, micronuclei in erythroblasts, micronuclei in leucopoietic cells, polyploid cells. One male animal each of the low-dose, the intermediate-dose and the high-dose group and one female animal of the intermediate dose group died in the course of the experiment. In all dosage groups the percentage of cells displaying anomalies of nuclei did not differ significantly from the negative control. By contrast, the positive control (cyclophosphamide, 128 mg/kg bw) yielded a marked increase of the percentage of cells with anomalies. Here the mean percentage of anomalies was 11.4, whereas the negative control yielded a percentage of 0.08.
Justification for selection of genetic toxicity endpoint
The in vivo study was selected, although all available studies are required to cover mutagenicity.
Short description of key information:
Ames test (Ciba-Geigy, 1981): negative
HPRT (BASG, 2012): negative
In vivo micronucleus test (Ciba-Geigy, 1983): negative
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
Dangerous Substance Directive (67/548/EEC)
-No classification required for genotoxicity.
Classification, Labeling, and Packaging Regulation (EC) No. 1272/2008:
- No classification required for genotoxicity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.