2-{4-[4-[4-fluoro-6-(2-(2-vinylsulfonylethoxy)ethylamino)-1,3,5-triazin-2-ylamino]phenylazo]phenylazo}naphthalene-4,6,8-trisulfonate, trisodium salt

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 August 2001 to 17 October 2001

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

other: HPLC estimation method

Specific details on test material used for the study:

Identity: FAT 40800/A
Batch number: WP 2/01
Active ingredient: ca. 70 %
Aggregate state at room temperature: Solid / powder
Color: Dark orange
Storage conditions: At room temperature at about 20 °C, away form direct sunlight
Expiration Date: February 14, 2007

Radiolabelling:

no

Test temperature:

25 °C

Details on study design: HPLC method:

TEST ITEM SOLUTION:
FAT 40'800/A (10.1 mg) was dissolved in methanol (25 mL) to prepare a stock solution of 404 µg/mL. 1 mL of the stock solution was diluted with a solvent mixture of methanol and water (55:45 v/v) to 10 mL to obtain a standard solution of 40.4 µg/mL. The pH value of the test item solution was determined to be 7.2. The test item solution was injected three times.

REFERENCE SOLUTIONS:
The reference items were chosen from the list given in the OECD Guideline No. 121. Stock solutions of the reference items in methanol were prepared as described: 23.7 mg of the reference item phenol, 21.2 mg of isoproturon, 23.7 mg of triadimenol, 22.4 mg of linuron, 23.2 mg of pyrazophos and 24.8 mg of diclofop-methyl were each dissolved in 25 mL methanol.
A combined reference solution was prepared by transferring 220 µL of the phenol, 250 µL of the isoproturon stock solution, 800 µL of the triadimenol stock solution, 140 µL of the linuron, 2 ml of the pyrazophos stock solution and 1 ml of the diclofop-methyl stock solution into one volumetric flask and diluted to a volume of 10 ml with eluent. This reference solution was injected six times.
Sodium nitrate (55 mg) was diluted to 50 mL with methanol to obtain a stock solution. The stock solution (50 pi) was diluted to 1 mL with a solvent mixture of methanol and water (55:45 v/v). The sodium nitrate solution was injected two times for the determination of the dead time of the HPLC system.

HPLC CONDITIONS:
This method utilises a high performance liquid Chromatograph with UV-VIS detector, reversed phase column and an aqueous mobile phase. Details:
- Apparatus:
- Varian Work Station
- Varian Pump ProStar 230
- Varian Autosampler 9100
- Varian Detector ProStar 310
- Jones Column Oven Mod. 7990
- Column: LiChrospher 100 CN, part, size 5 µm, 250 mm · 4 mm
- Mobile Phase: Methanol/Water (55:45 v/v); pH 7.5
- Detection: 224 nm for the test item and the blank solution, 210 nm for the reference items the sodium nitrate solution and the blank solution
- Temperature: 25 °C
- Injection Volume: 50 µL
- Flow: 1.0 mL/min

Type:

log Koc

Value:

< 1.32

Validity criteria fulfilled:

yes

Conclusions:

The log Koc of the test substance was estimated to be <1.32 which is equal to a Koc value of <21. This value indicates a very mobile character of the test substance.

Executive summary:

The determination of the adsorption coefficient (Koc) of the test substance was performed according to the OECD Guideline for the Testing of Chemicals, Estimation of the Adsorption Coefficient (Koc) on Soil and Sewage Sludge using High Performance Liquid Chromatography (HPLC), Draft Guideline (June 2000) under GLP compliance. Using the corresponding log Koc values of the reference items from the OECD guideline, the regression coefficients were calculated. The log Koc value of the test substance was calculated from the calibration equation. The adsorption coefficient (Koc) of the test substance was estimated to be log Koc <1.32 which is equal to a Koc value of <21. This value indicates a mobile character of the test substance.

Description of key information

With a log Koc of <1.32 L/kg (equivalent to a Koc value of <21), the substance is be considered to bemobile.

Key value for chemical safety assessment

Koc at 20 °C:

21

Additional information

The determination of the adsorption coefficient (Koc) of the test substance was performed according to the OECD Guideline for the Testing of Chemicals, Estimation of the Adsorption Coefficient (Koc) on Soil and Sewage Sludge using High Performance Liquid Chromatography (HPLC), Draft Guideline (June 2000) under GLP-compliance. Using the corresponding log Koc values of the reference items from the OECD guideline, the regression coefficients were calculated. The log Koc value of the test substance was calculated from the calibration equation. The adsorption coefficient (Koc) of the test substance was estimated to be log Koc <1.32 which is equal to a Koc value of <21. This value indicates a mobile character of the test substance.