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EC number: 421-320-0 | CAS number: 118289-55-7 CP-89,575
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 5 March 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: done under GLP and OECD method
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Principles of method if other than guideline:
- The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex
to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 2 "Acute toxicity to Daphnia" and the OECD Guideline for Testing of Chemicals No. 202, Part I "Daphnia Acute Immobilisation Test".
Test was assessed under static exposure conditions. - GLP compliance:
- yes
Test material
- Reference substance name:
- 6-chloro-5-(2-chloroethyl)-1,3-dihydroindol-2-one
- EC Number:
- 421-320-0
- EC Name:
- 6-chloro-5-(2-chloroethyl)-1,3-dihydroindol-2-one
- Cas Number:
- 118289-55-7
- Molecular formula:
- Hill formula: C10 H9 Cl2 N O CAS formula: C10 H9 Cl2 N O
- IUPAC Name:
- 6-chloro-5-(2-chloroethyl)-2,3-dihydro-1H-indol-2-one
- Test material form:
- not specified
- Details on test material:
- Expiry: 2 June 1999
Purity: 98.82% by hplc
Date received: 2 June 1997
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- TEST SUBSTANCE PREPARATION: concentrations were verified by chemical analysis. Samples, 100 ml, were taken from the pooled replicates of control and each test concentration at 0 hours and after 48 hours
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test organisms were maintained and the tests conducted in a reconstituted medium Elendt M4. The medium was prepared using analytical grade reagents and deionised water. Appendix 2 gives full details of the medium
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain:Straus
- Source:cultured in-house and were obtained from a strain originating from the Institute National de Recherche Chimique Applique (IRChA), France
- Age at study initiation (mean and range, SD):less than 24 hours old
ACCLIMATION
- Acclimation period:None
Stock cultures of Daphnia magna were maintained in glass vessels containing approximately 300 ml of Elendt M4 culture medium with a 16 hour light: 8 hour dark photoperiod at 20 ± 2°C. Cultures were fed daily with a suspension of the unicellular green alga, Chiorella vulgaris. Culture conditions ensure that cultures reproduce by parthenogenesis.
The day before the start of the study, all juvenile Daphnia were removed from the laboratory cultures. The following morning, juveniles produced by the gravid (egg-bearing) adult Daphnia were used in the test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- without renewal of test media
- Post exposure observation period:
- The temperature in each vessel was measured daily and the pH and dissolved oxygen levels recorded at the start and at the end of the study
Test conditions
- Hardness:
- No Data
- Test temperature:
- 20 ± 1 °C
- pH:
- pH 7.8
- Dissolved oxygen:
- 6.8-8.4 mg/l
- Nominal and measured concentrations:
- The following test concentrations were prepared based on the results of the earlier range finding study.
Mean measured test concentrations: 0.044, 0.098, 0.23, 0.49, 1.1, 2.3 and 5.0 mg/L
Nominal exposure concentrations quoted in this report refer to the test material as received; no allowance has been made for a purity of less than 100%. - Details on test conditions:
TEST SYSTEM
- Test vessel:250 ml capacity glass jars.
- Type (delete if not applicable): loosely covered to minimise evaporative losses
- Material, size, headspace, fill volume:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel:
- No. of vessels per concentration (replicates):
- No. of vessels per control (replicates):
- No. of vessels per vehicle control (replicates):
- Biomass loading rate:Ten first instar Daphnia were placed without conscious bias into glass jars
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:with a photoperiod of 16 hours light: 8 hours dark and without supplementary aeration or feeding
- Light intensity:
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations:
- Results used to determine the conditions for the definitive study:- Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 5 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.51 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 0.34-0.75 (mg/l)
- Details on results:
- Highest test concentration resulting in 0% immobilisation: <0.044 mg/1*
Lowest test concentration resulting in 100% immobilisation:5.0 mg/1
*A 10% immobilisation was observed in the control and lowest test level. However, a 10% immobilisation is not considered to be biologically significant, and the "No observed effect concentration" is assumed to be 0.044 mg/1.
Any other information on results incl. tables
Summary of chemical analysis results
Occasion |
Nominal concentration(mgll) |
Measured concentration(mg/1) |
Achieved dilutionfactor(%)! |
%oftarget dilutionfactor |
0hours |
Control 1.0 2.2 4.6 10 22 46 100 |
ND 0.04580 0.1057 0.2285 0.4999 1.105 2.293 4.945 |
- 0.916 2.11 4.57 10.0 22.1 45.9 98.9 |
- 91.6 96.1 99.3 100 100 99.7 98.9 |
48 hours |
Control 1.0 2.2 4.6 10 22 46 100 |
ND 0.04264 0.08979 0.2345 0.4809 1.109 2.312 5.007 |
- 0.853 1.80 4.69 9.62 22.2 46.2 100 |
- 85.3 81.6 102 96.2 101 101 100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 h EC50 (immobilisation value for CP-89,575 with Daphnia magna) was determined to be 0.51 mg/1. The 'No-observed effect concentration' (NOEC) was 0.044 mg/1.
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