(undec-10-enoylamino)acetic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 2008-09-11 to 2008-11-12

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study, OECD guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

2007-01-12

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

As solubility surpasses 100mg/L, a solution containing 100 mg/L in dilution water was prepared. The resulting solution was clear and homogeneous. The pH of the solution was determined as 6.5.

Test organisms (species):

Daphnia magna

Details on test organisms:

Variety STRAUS
Strain: Berlin
Sex: female
Age: between 0 end 24 hours
Origin: Umweltbundesamt Berlin
Arrival of Strain: September, 27th 2007
Selection of the test system was made following the proposal of the guidelines.
Food: unicellular green algae, not during the test
Medium renewal: twice a week
Photo period: 16/8 hours, using neon tubes
T: 20 +/- 2°C

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

2.50 mmol/L

Test temperature:

23.5 - 24.1 °C

pH:

from 7.2 to 7.7

Dissolved oxygen:

above 8.3 mg/L

Details on test conditions:

- Vessels: glass beakers, nominal volume 50 mL, tall shape
- Replicates: four vessels, each containing 20 mL test solution and five daphnia
- Control: four vessels, each containing 20 mL dilution water and five daphnia
- Feeding: none
- lighting: none
-Temperature: 20 +/-2°C

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

24h EC50 of K2Cr2O7 was determined at 0.79mg/L
Immobilisation in the controls was 10% at he most
The lowest concentration of dissolved oxygen at the end of the test was 8.4mg/L
The highest pH variation was 0.7 units.
The study can be considered valid

Results with reference substance (positive control):

24h EC50 of K2Cr2O7 was determined at 0.79 mg/L

Validity criteria fulfilled:

yes

Conclusions:

24h NOEC= 100mg/L
48h NOEC= 100mg/L
24h EC50i> 100mg/L
48h EC50> 100 mg/L

Executive summary:

This study was performed in order to evaluate the toxic potential of Lipacide UG towards freshwater shrimp, using species Daphnia magna. D magna STRAUSS, which belongs to the family of crustacea, was chosen in the guideline as a typical part of zoo-plancton.

Twenty daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.

No animals were immobilised in the control. 10% of the animals were immobilised in the treatment, which was stated as not significant.

The 24h-EC50i of potassium dichromate was tested in a current reference test, the value was determined at 0.79mg/L, lying within the demanded range of 0.6 -2.1 mg/L.

at the start and at the end of the test, the content of the test item in the test solutions was determined. the recovery after 48 hours was 97% of the start concentration, the correlation between nominal and measured concentration was very good. Therefore, the determination of the biological results was based on the nominal concentration.

24h NOEC= 100mg/L

48h NOEC= 100mg/L

24h EC50_i > 100mg/L

48h EC50_i > 100 mg/L

Description of key information

In order to evaluate the toxic potential of the test substance towards freshwater shrimp, using species Daphnia magna an OECD Guideline 202( Daphnia sp.Acute Immobilisation test was performed towards freshwater shrimp.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L