(Benzyloxycarbonyl)methyl 4-(1-Butyl-5-cyano-1,6-dihydro-2-hydroxy-4-methyl-6-oxo-3-pyridylazo)-benzoate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From October 28th, 2002 to November 1st, 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

1984

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1992

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Yellow 6314-PPT

IUPAC Name:

Yellow 6314-PPT

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Due to the low water solubility of the test item, a supersaturated dispersion of the test item with a loading rate of 100 mg/l was continuously stirred at room temperature in the dark for 3 hours. Then the dispersion was filtered. Only the undiluted filtrate with the maximum concentration of dissolved test item was used as test medium. Additionally, a control was tested in parallel.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

20 daphnids tested per control
20 daphnids tested per undiluted filtrate

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

250.0 mg CaCO3 /l

Test temperature:

20 °C

pH:

7.8 control
7.7 - 7.9 undiluted filtrate

Dissolved oxygen:

8.3-8.8 mg/l control
8.3-8.4 mg/l undiluted filtrate

Nominal and measured concentrations:

Nominal 100 mg/l as loading rate

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The 48-hour NOEC and the 48-hour EC0 of the test item to Daphnia magna were determined to be at least at a loading rate of 100 mg/l. The 48-hour NOEC and the 48 hour EC0 might even be higher, but concentrations in excess of the loading rate of 100 mg/l have not been tested, due to the low water solubility of the test item in the used test water.

Applicant's summary and conclusion

Conclusions:

The test item had no toxic effect on the daphnia up to its solubility limit in test water.