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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From July 15th, 2002 to August 15th, 2002
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Version / remarks:
1992
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Version / remarks:
1992
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Yellow 6314-PPT
IUPAC Name:
Yellow 6314-PPT
Test material form:
solid

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Duration of test (contact time):
28 d
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradationopen allclose all
Parameter:
other: % biodegradation = 100 × BOD / ThODNH4
Remarks:
Without nitrification
Value:
3
Sampling time:
28 d
Parameter:
other: % biodegradation = 100 × BOD / ThODNO3
Remarks:
With nitrification
Value:
2
Sampling time:
28 d
Details on results:
Points of degradation plot (test substance) based on ThOD_NH4:
1.5 % degradation after 7 d
2.5 % degradation after 14 d
3 % degradation after 21 d
3 % degradation after 27 d
3 % degradation after 28 d

Points of degradation plot (test substance) based on ThOD_NO3:
1 % degradation after 7 d
2 % degradation after 14 d
2 % degradation after 21 d
2 % degradation after 27 d
2 % degradation after 28 d

BOD5 / COD results

BOD5 / CODopen allclose all
Parameter:
ThOD
Value:
1.75 g O2/g test mat.
Remarks on result:
other: without nitrification
Parameter:
ThOD
Value:
2.26 g O2/g test mat.
Remarks on result:
other: with nitrification
Results with reference substance:
Points of degradation plot (reference substance) based on ThOD:
79 % degradation after 7 d
85.5 % degradation after 14 d
88.5 % degradation after 21 d
90 % degradation after 27 d
90 % degradation after 28 d

Any other information on results incl. tables

Biodegradadtion of reference item was 85.5 % at day 14, thus confirming the suitability of activated sludge. At the end of the test on day 28, degradation was 90 %.

The test item has no inhibitory effect on activated sludge microorganisms, as biodegradation rate in the toxicity control was > 25 % within 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
The test item was found to be not biodegradable under the test consitions within 28 days.