Ethyltriphenylfosfonium bromide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material : Ethyltriphenylfosfonium bromide
- Molecular formula : C20H20P.Br
- Molecular weight : 371.256 g/mol
- Smiles notation : [P+](c1ccccc1)(c1ccccc1)(c1ccccc1)CC.[BrH-]
- InChl : 1S/C20H20P.BrH/c1-2-21(18-12-6-3-7-13-18,19-14-8-4-9-15-19)20-16-10-5-11-17-20;/h3-17H,2H2,1H3;1H/q+1;/p-1
- Substance type: Organic
- Physical state: Solid
- Physical Appearance: White crystalline powder
-Purity: 99.7%
-Batch no.: ETPB1/B/14016

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

The test substance was soluble in water. Initially, stock solution was prepared by dissolving 1g of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous stirring. From this stock solution, further test concentration was prepared for achieving test concentrations of 18 mg/L, 23.4 mg/L, 30.42 mg/L, 39.54 mg/L & 51.4 mg/L, respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Orange City Aquarium, Sitabuldi, Nagpur, MS (India)
- Length at study initiation (length definition, mean, range and SD): average 2.7 cm
- Weight at study initiation (mean and range, SD): average 0.272 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12 hours darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

50.75 mg of CaCO3

Test temperature:

23°C

pH:

6.91

Dissolved oxygen:

7.2 mg/l

Nominal and measured concentrations:

18 mg/L, 23.4 mg/L, 30.42 mg/L, 39.54 mg/L & 51.4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

23.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 39.42 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

51.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

8.1. Mortality and symptoms:

NominalConcentration (mg/l)	Number of fish	Mortality (cumulated) after
		24h	48h	72h	96h
0 (control)	8	-	-	-	-
18	8	-	-	-	-
23.4	8	-	-	-	-
30.42	8	-	-	1	1
39.54	8	-	1	1	1
51.4	8	2	6	-	-

NominalConcentration(mg/l)	Number of fish	Symptoms after
		24h	48h	72h	96h
0 (control)	8	NS8	NS8	NS8	NS8
18	8	M8	M8	M8	M8
23.4	8	M8	M8	M8	M8
30.42	8	M8	M8	M7	M6
39.54	8	M8	M7	M6	M5
51.4	8	M6	-	-	-

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish.

8.2. pH and oxygen content:

NominalConcentration(mg/l)	pH readings after
	24h	48h	72h	96h
0 (control)	7.4	7.2	7.2	6.5
18	6.7	6.5	6.0	6.0
23.4	6.6	6.5	6.4	6.1
30.42	6.6	6.6	6.4	6.0
39.54	6.7	6.6	6.6	6.1
51.4	6.7	6.6	-	-

NominalConcentration(mg/l)	Oxygen content (mg/l) after
	24h	48h	72h	96h
0 (control)	6.5	6.5	6.2	6.1
18	6.5	6.5	6.2	6.1
23.4	6.5	6.5	6.3	6.2
30.42	6.6	6.6	6.2	6.1
39.54	6.6	6.6	6.3	6.2
51.4	6.7	6.6	-	-

8.3. Test temperature

NominalConcentration(mg/l)	Temperature (°C) after
	24h	48h	72h	96h
0 (control)	23	23	23	23
18	23	23	23	23
23.4	23	23	23	23
30.42	23	23	23	23
39.54	23	23	23	23
51.4	23	23	-	-

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish Danio rerio was determine to be >39.42 mg/L. LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 51.4 mg/L.

Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Initially, stock solution was prepared by dissolving 1g of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous stirring. From this stock solution, further test concentration was prepared for achieving test concentrations of 18 mg/L, 23.4 mg/L, 30.42 mg/L, 39.54 mg/L & 51.4 mg/L, respectively. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations 18 mg/L, 23.4 mg/L, 30.42 mg/L, 39.54 mg/L & 51.4 mg/l

LC50 was determine to be >39.42 mg/l . Based on the LC50, LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 51.4 mg/L it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Initially, stock solution was prepared by dissolving 1g of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous stirring. From this stock solution, further test concentration was prepared for achieving test concentrations of 18 mg/L, 23.4 mg/L, 30.42 mg/L, 39.54 mg/L & 51.4 mg/L, respectively.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations18 mg/L, 23.4 mg/L, 30.42 mg/L, 39.54 mg/L & 51.4 mg/l

LC50 was determine to be >39.42 mg/l . Based on the LC50,LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 51.4 mg/Lit can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

39.42 mg/L

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Initially, stock solution was prepared by dissolving 1g of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous stirring. From this stock solution, further test concentration was prepared for achieving test concentrations of 18 mg/L, 23.4 mg/L, 30.42 mg/L, 39.54 mg/L & 51.4 mg/L, respectively.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations18 mg/L, 23.4 mg/L, 30.42 mg/L, 39.54 mg/L & 51.4 mg/l

LC50 was determine to be >39.42 mg/l . Based on the LC50,LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 51.4 mg/Lit can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.