Registration Dossier
Registration Dossier
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EC number: 215-385-2 | CAS number: 1324-76-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 02.1993-03.1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study was performed according to test guideline and in compliant with GLP for Pigment Blue 56, which is structurally similar to Pigment Blue 61. Thus the study is used for read-across to avoid duplicate tests.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-methyl-4-[[4-[[4-[(3-tolyl)amino]phenyl][4-[(3-tolylimino]-2,5-cyclohexadien-1-ylidene]methyl]phenyl]amino]benzenesulphonic acid
- EC Number:
- 229-139-7
- EC Name:
- 2-methyl-4-[[4-[[4-[(3-tolyl)amino]phenyl][4-[(3-tolylimino]-2,5-cyclohexadien-1-ylidene]methyl]phenyl]amino]benzenesulphonic acid
- Cas Number:
- 6417-46-5
- IUPAC Name:
- 2-methyl-4-{[4-({4-[(3-methylphenyl)amino]phenyl}{4-[(3-methylphenyl)imino]cyclohexa-2,5-dien-1-ylidene}methyl)phenyl]amino}benzenesulfonic acid
- Reference substance name:
- Pigment Blue 56
- IUPAC Name:
- Pigment Blue 56
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- HOE CG-Nr.: 0432 0D ZD24 0001
Charge: 21/29
Molecular formular: C40H36N3SO3
Molecular wiehgt: 638
Epx date: February 1997
Purity 24.2% (m/m) Certificate 11.06.1992 Nr.: 04737
Constituent 1
Constituent 2
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, non-adapted
- Details on inoculum:
- supernatant water of activated sludge from the domestic sewage: Frankfurt-Sindling was used according to EG 88/302/EWG 1.6.3
7 days oxygeneatad for depletion of carbon in the activated sluge - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- ca. 5.33 mg/L
- Based on:
- DOC
- Initial conc.:
- ca. 5.06 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 1.9 - <= 2.3
- Sampling time:
- 3 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 4.2 - <= 4.6
- Sampling time:
- 7 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 5.3 - <= 7.1
- Sampling time:
- 14 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 6.4 - <= 9.8
- Sampling time:
- 21 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 15.9 - <= 22.7
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- degradation 83.2% after 21 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- It is not readily biodegradable
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