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EC number: 421-750-9 | CAS number: 57280-22-5 TRIOXABICYCLOOCTAN; TRIOXABICYCLOOCTANE
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March to June 1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- 24 February 1987
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4,4-dimethyl-3,5,8-trioxabicyclo[5.1.0]octane
- EC Number:
- 421-750-9
- EC Name:
- 4,4-dimethyl-3,5,8-trioxabicyclo[5.1.0]octane
- Cas Number:
- 57280-22-5
- Molecular formula:
- C7H12O3
- IUPAC Name:
- 4,4-dimethyl-3,5,8-trioxabicyclo[5.1.0]octane
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Schriever
- Age at study initiation: adults
- Weight at study initiation: male: 2.6 kg; females: 3.1 kg
- Housing: individually in conventional metal cages
- Diet (e.g. ad libitum): pell. Altromin® K; ad libitum
- Water (e.g. ad libitum): demineralized water; ad libitum
- Acclimation period: 18 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21
- Humidity (%):52-58
- Photoperiod (hrs dark / hrs light): 12/12
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.1 mL
- Concentration (if solution): 100 % a.i.
- Duration of treatment / exposure:
- single exposure and observation 0.5, 1 and 2 h after application and once daily afterwards until day 16
- Observation period (in vivo):
- 16 days
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- SCORING SYSTEM: According to OECD test guideline 405
TOOL USED TO ASSESS SCORE: hand-slit lamp / biomicroscope / fluorescein: not specified
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- chemosis score
- Basis:
- animal: #2 #3
- Time point:
- 24/48/72 h
- Score:
- 3
- Max. score:
- 4
- Reversibility:
- fully reversible within: 16 days
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 2.67
- Max. score:
- 4
- Reversibility:
- fully reversible within: 16 days
- Irritation parameter:
- conjunctivae score
- Basis:
- animal: #2 #3
- Time point:
- 24/48/72 h
- Score:
- 2.67
- Max. score:
- 3
- Reversibility:
- fully reversible within: 16 days
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 1.3
- Max. score:
- 3
- Reversibility:
- fully reversible within: 16 days
- Irritation parameter:
- iris score
- Basis:
- animal: #1 - #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 2
- Reversibility:
- fully reversible within: 16 days
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: #2 #3
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 16 days
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 1.67
- Max. score:
- 4
- Reversibility:
- fully reversible within: 16 days
Any other information on results incl. tables
Test substance/ Calculation for EEC classification | ||||
Location (finding) | Time point after end of exposure time | 690M | 702F | 704F |
Cornea (opacity | 24 h | 1.0 | 1.0 | 1.0 |
48 h | 2.0 | 1.0 | 1.0 | |
72 h | 2.0 | 1.0 | 1.0 | |
Individual mean values |
| 1.67 | 1.0 | 1.0 |
| ||||
iris | 24 h | 1.0 | 1.0 | 1.0 |
48 h | 1.0 | 1.0 | 1.0 | |
72 h | 1.0 | 1.0 | 1.0 | |
Individual mean values |
| 1.0 | 1.0 | 1.0 |
| ||||
Conjunctivae (reddening) | 24 h | 2.0 | 3.0 | 3.0 |
48 h | 3.0 | 3.0 | 3.0 | |
72 h | 3.0 | 3.0 | 3.0 | |
Individual mean values |
| 2.67 | 3.0 | 3.0 |
| ||||
Conjunctivae (swelling) | 24 h | 2.0 | 3.0 | 3.0 |
48 h | 1.0 | 3.0 | 3.0 | |
72 h | 1.0 | 2.0 | 2.0 | |
Individual mean values |
| 1.3 | 2.67 | 2.67 |
|
Applicant's summary and conclusion
- Interpretation of results:
- Category 2 (irritating to eyes) based on GHS criteria
- Conclusions:
- On the whole, a single application of Trioxabicyclooctan into the conjunctival sac of the rabbit eye provoked severe irritation which lasted for several days after application and which only very slowly normalized over a period of up to 16 days.
Although the rabbit eye reacts more sensitively than the human eye due to anatomical and physiological. differences, similar effects have to be expected on the human eye. Therefore, inadvertent contact of the human eye with this substance should be strictly avoided. - Executive summary:
In a primary eye irritation study according to OECD test guideline 405 (1987), 100 µL Trioxabicyclooctan (100 % a.i.) were instilled into the conjunctival sac of one eye of 3 young adult New Zealand White rabbits. Animals then were observed for 16 days. Irritation was scored by the method recommended by the test guideline.
After application of 100 µL of the test sample into the conjunctival sac, there were slight reactions in the cornea and initially strong changes in the conjunctivae, which are completely reversible within 16 days. According to Regulation (EU) No. 1272/2008 (CLP) the test item is classified as Category 2 (irritating to eyes) based on the results obtained under the conditions described.
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