3-(diethylamino)-7-imino-7H-[1]benzopyrano[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile

Endpoint summary

Description of key information

Acute oral toxicity:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the acute oral toxicity of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS no.: 52372-39-1). The studies are as mentioned below:

1. The acute oral toxicity study was conducted by using test chemical in 10 Sprague-Dawley (albino) male rats at the concentration of 5000 mg/kg bw. The test material was dosed as a 25% w/v suspension in corn oil and administered via oral gavage route. Animals were observed over 14 days, several times during the first day, and once daily thereafter. No mortality was observed in treated rats at 5000 mg/kg bw. Therefore, LD50 was considered to be >5000 mg/kg bw, when 10 Sprague-Dawley (albino) male rats were treated with test chemical via oral gavage route.

2. The acute oral toxicity study was conducted by using test chemical in rats at the concentration of 5000 mg/kg bw. 50% mortality was observed in treated rats. Therefore, LD50 was considered to be 5000 mg/kg bw, when rats were treated with test chemical via oral route.

Thus, based on the above summarised studies, 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS no.: 52372-39-1) and it’s structurally similar read across substances, it can be concluded that LD50 value is >2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS no.: 52372-39-1) cannot be classified for acute oral toxicity. Hence, 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile is not toxic for acute oral toxicity.

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

experimental data of read across substances

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

WoE report is based on 2 acute oral toxicity studies as- WoE-2 and WoE-3.
Acute Oral toxicity test was carried out to study the effects of the test chemicals on rodents.

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Specific details on test material used for the study:

Name: 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile
InChI:1S/C23H19N5O/c1-3-27(4-2)15-10-9-14-11-16-21(29-20(14)12-15)17(13-24)22(25)28-19-8-6-5-7-18(19)26-23(16)28/h5-12,25H,3-4H2,1-2H3
Smiles:c12cc3c4nc5ccccc5n4c(=N)c(C#N)c3oc1cc(N(CC)CC)cc2
Molecular Formula: C23H19N5O
Molecular Weight: 381.437 g/mole

Species:

rat

Strain:

other: 1. Sprague-Dawley Rat (albino) 2. not specified

Sex:

male

Details on test animals or test system and environmental conditions:

1. TEST ANIMALS
- Weight at study initiation: 219 – 240 grams
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
2. not specified

Route of administration:

other: 1. oral: gavage 2. oral: unspecified

Vehicle:

other: 1. corn oil 2. not specified

Details on oral exposure:

1. VEHICLE
- Amount of vehicle (if gavage): 25% w/v suspension in corn oil.
2. not specified

Doses:

1. 5000 mg/kg bw
2. 5000 mg/kg bw

No. of animals per sex per dose:

1. 10 male rats
2. not specified

Control animals:

not specified

Details on study design:

1. - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Animals were Observed over 14 days, several times during the first day, and once daily thereafter.
2. not specified

Statistics:

1. not specified
2. not specified

Preliminary study:

1. not specified
2. not specified

Sex:

male

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: No mortality was observed

Sex:

not specified

Dose descriptor:

LD50

Effect level:

5 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: 50% mortality was observed

Mortality:

1. No mortality was observed in treated rats at 5000 mg/kg bw
2. 50% mortality was observed in treated rats at 5000 mg/kg bw

Clinical signs:

other: 1. not specified 2. not specified

Gross pathology:

1. not specified
2. not specified

Other findings:

1. not specified
2. not specified

Interpretation of results:

other: Not classified

Conclusions:

According to CLP regulation the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS no.: 52372-39-1) cannot be classified for acute oral toxicity, as the LD50 value is >2000 mg/kg bw.

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the acute oral toxicity of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS no.: 52372-39-1). The studies are as mentioned below:

1. The acute oral toxicity study was conducted by using test chemical in 10 Sprague-Dawley (albino) male rats at the concentration of 5000 mg/kg bw. The test material was dosed as a 25% w/v suspension in corn oil and administered via oral gavage route. Animals were observed over 14 days, several times during the first day, and once daily thereafter. No mortality was observed in treated rats at 5000 mg/kg bw. Therefore, LD50 was considered to be >5000 mg/kg bw, when 10 Sprague-Dawley (albino) male rats were treated with test chemical via oral gavage route.

2. The acute oral toxicity study was conducted by using test chemical in rats at the concentration of 5000 mg/kg bw. 50% mortality was observed in treated rats. Therefore, LD50 was considered to be 5000 mg/kg bw, when rats were treated with test chemical via oral route.

Thus, based on the above summarised studies, 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS no.: 52372-39-1) and it’s structurally similar read across substances, it can be concluded that LD50 value is >2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS no.: 52372-39-1) cannot be classified for acute oral toxicity. Hence, 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile is not toxic for acute oral toxicity.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Dose descriptor:

LD50

Value:

5 000 mg/kg bw

Quality of whole database:

Data is Klimisch 2 and from report.

Acute toxicity: via inhalation route

Endpoint conclusion

Endpoint conclusion:

no study available

Acute toxicity: via dermal route

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Acute oral toxicity:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the acute oral toxicity of the test chemical 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS no.: 52372-39-1). The studies are as mentioned below:

1. The acute oral toxicity study was conducted by using test chemical in 10 Sprague-Dawley (albino) male rats at the concentration of 5000 mg/kg bw. The test material was dosed as a 25% w/v suspension in corn oil and administered via oral gavage route. Animals were observed over 14 days, several times during the first day, and once daily thereafter. No mortality was observed in treated rats at 5000 mg/kg bw. Therefore, LD50 was considered to be >5000 mg/kg bw, when 10 Sprague-Dawley (albino) male rats were treated with test chemical via oral gavage route.

2. The acute oral toxicity study was conducted by using test chemical in rats at the concentration of 5000 mg/kg bw. 50% mortality was observed in treated rats. Therefore, LD50 was considered to be 5000 mg/kg bw, when rats were treated with test chemical via oral route.

Thus, based on the above summarised studies, 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS no.: 52372-39-1) and it’s structurally similar read across substances, it can be concluded that LD50 value is >2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS no.: 52372-39-1) cannot be classified for acute oral toxicity. Hence, 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile is not toxic for acute oral toxicity.

Justification for classification or non-classification

Based on the above experimental studies on 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile (CAS no.: 52372-39-1) and it’s structurally similar read across substances, it can be concluded that LD50 value is >2000 mg/kg bw, for acute oral toxicity. Thus, comparing this value with the criteria of CLP regulation, 3-(diethylamino)-7-imino-7H-chromeno[3',2':3,4]pyrido[1,2-a]benzimidazole-6-carbonitrile cannot be classified for acute oral toxicity.