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EC number: 233-143-4 | CAS number: 10043-84-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical. The study was performed as per the plate incorporation protocol and the test chemical dissolved in 0.067M sodium phosphate buffer and was used at dose levels of 0, 0.033, 0.10, 0.33, 1.0, 3.3 and 10 mg per plate. Concurrent solvent and positive controls were run with the test chemical. Test results were considered valid only if the positive control compounds induced increases in mutant counts to at least twice background. The test chemical did not induce mutation in the Salmonella typhimurium strain TA98, TA100, TA1535, TA1537 and TA1538 and Escherichia coli strain WP2 with and without rat liver S9 mix and hence the chemical is negative for gene mutation in vitro.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed publication
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- Gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
- Target gene:
- Histidine for Salmonella and tryptophan for E. coli
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537 and TA1538
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- other: Histidine auxotroph
- Species / strain / cell type:
- E. coli WP2
- Details on mammalian cell type (if applicable):
- No data
- Additional strain / cell type characteristics:
- other: Tryptophan auxotroph
- Cytokinesis block (if used):
- No data
- Metabolic activation:
- with and without
- Metabolic activation system:
- 10% Aroclor 1254-induced liver S9 from male Sprague-Dawley rats.
- Test concentrations with justification for top dose:
- 0, 0.033, 0.10, 0.33, 1.0, 3.3 and 10 mg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: 0.067 M sodium phosphate buffer
- Justification for choice of solvent/vehicle: The chemical was soluble in sodium phosphate buffer - Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Sodium phosphate buffer
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- other: AF-2 (furylfuramide) or N-methyl-N'-nitro-N-nitrosoguanidine (E. coli WP2;without S9), -Anthramine (All strains; with S9)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: No data
- Exposure duration: No data
- Expression time (cells in growth medium): No data
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells): No data
SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data
NUMBER OF REPLICATIONS: All platings were performed in duplicate and all tests were repeated.
NUMBER OF CELLS EVALUATED: No data
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data
OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data
- Other: No data
OTHER: No data - Rationale for test conditions:
- No data
- Evaluation criteria:
- Test results were considered valid only if the positive control compounds induced increases in mutant counts to at least twice background.
- Statistics:
- None--straight counting of colonies on plates.
- Species / strain:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537 and TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Effects of pH: No data
- Effects of osmolality: No data
- Evaporation from medium: No data
- Water solubility: No data
- Precipitation: No data
- Definition of acceptable cells for analysis: No data
- Other confounding effects: No data
RANGE-FINDING/SCREENING STUDIES: The test compound was first tested in a range finding assay at dose levels between 0.3 and 10000 µg/plate
CYTOKINESIS BLOCK (if used)
- Distribution of mono-, bi- and multi-nucleated cells: No data
NUMBER OF CELLS WITH MICRONUCLEI
- Number of cells for each treated and control culture: No data
- Indication whether binucleate or mononucleate where appropriate: No data
HISTORICAL CONTROL DATA (with ranges, means and standard deviation and confidence interval (e.g. 95%)
- Positive historical control data: No data
- Negative (solvent/vehicle) historical control data: No data
ADDITIONAL INFORMATION ON CYTOTOXICITY:
- Measurement of cytotoxicity used: No data
- Other observations when applicable: No data - Remarks on result:
- other: No mutagenic potential
- Conclusions:
- The test chemical did not induce mutation in the Salmonella typhimurium strain TA98, TA100, TA1535, TA1537 and TA1538 and Escherichia coli strain WP2 with and without rat liver S9 mix and hence the chemical is negative for gene mutation in vitro.
- Executive summary:
Gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical. The study was performed as per the plate incorporation protocol and the test chemical dissolved in 0.067M sodium phosphate buffer and was used at dose levels of 0, 0.033, 0.10, 0.33, 1.0, 3.3 and 10 mg per plate. Concurrent solvent and positive controls were run with the test chemical. Test results were considered valid only if the positive control compounds induced increases in mutant counts to at least twice background. The test chemical did not induce mutation in the Salmonella typhimurium strain TA98, TA100, TA1535, TA1537 and TA1538 and Escherichia coli strain WP2 with and without rat liver S9 mix and hence the chemical is negative for gene mutation in vitro.
Reference
Table: In vitro assay results
Compound |
Metabolic activation |
Dose (µg/plate) |
Histidine revertants/plate TA1535 |
|||||
Experiment 1 |
Experiment 2 |
|||||||
|
|
Average |
|
|
Average |
|||
Negative control |
- |
|
16 |
18 |
20 |
18 |
15 |
24 |
- |
|
26 |
21 |
34 |
29 |
|||
+ |
|
16 |
15 |
15 |
14 |
13 |
10 |
|
+ |
|
14 |
14 |
6 |
8 |
|||
Positive control |
|
|
|
|
|
|
|
|
Sodium azide |
- |
1.0 |
91 |
103 |
97 |
382 |
435 |
409 |
2-Anthramine |
- |
2.5 |
25 |
19 |
22 |
24 |
20 |
22 |
+ |
2.5 |
304 |
281 |
293 |
322 |
325 |
324 |
|
Manganese hypophosphite |
- |
33.0 |
15 |
20 |
18 |
17 |
26 |
22 |
- |
100.0 |
27 |
24 |
26 |
19 |
25 |
22 |
|
- |
333.3 |
18 |
26 |
22 |
18 |
14 |
16 |
|
- |
1000.0 |
17 |
31 |
24 |
21 |
24 |
23 |
|
- |
3333.3 |
25 |
24 |
25 |
20 |
21 |
21 |
|
- |
10000.0 |
19 |
13 |
16 |
20 |
19 |
20 |
|
+ |
33.0 |
17 |
18 |
18 |
9 |
10 |
10 |
|
+ |
100.0 |
20 |
18 |
19 |
10 |
12 |
11 |
|
+ |
333.3 |
18 |
15 |
17 |
14 |
10 |
12 |
|
+ |
1000.0 |
13 |
14 |
14 |
10 |
13 |
12 |
|
+ |
3333.3 |
19 |
18 |
19 |
7 |
12 |
10 |
|
+ |
10000.0 |
21 |
11 |
16 |
14 |
7 |
11 |
Compound |
Metabolic activation |
Dose (µg/plate) |
Histidine revertants/plate TA1537 |
|||||
Experiment 1 |
Experiment 2 |
|||||||
|
|
Average |
|
|
Average |
|||
Negative control |
- |
|
8 |
7 |
7 |
10 |
6 |
6 |
- |
|
6 |
8 |
2 |
4 |
|||
+ |
|
6 |
7 |
10 |
9 |
15 |
11 |
|
+ |
|
12 |
16 |
10 |
11 |
|||
Positive control |
|
|
|
|
|
|
|
|
9- Aminoacridine |
- |
50.0 |
201 |
195 |
198 |
306 |
269 |
288 |
2-Anthramine |
- |
2.5 |
20 |
6 |
13 |
7 |
8 |
8 |
+ |
2.5 |
190 |
220 |
205 |
89 |
77 |
83 |
|
Manganese hypophosphite |
- |
33.0 |
8 |
7 |
8 |
6 |
7 |
7 |
- |
100.0 |
2 |
3 |
3 |
6 |
11 |
9 |
|
- |
333.3 |
6 |
8 |
7 |
10 |
14 |
12 |
|
- |
1000.0 |
5 |
8 |
7 |
7 |
9 |
8 |
|
- |
3333.3 |
8 |
10 |
9 |
6 |
7 |
7 |
|
- |
10000.0 |
7 |
5 |
6 |
9 |
8 |
9 |
|
+ |
33.0 |
8 |
8 |
8 |
12 |
15 |
14 |
|
+ |
100.0 |
8 |
15 |
12 |
20 |
17 |
19 |
|
+ |
333.3 |
14 |
7 |
11 |
8 |
11 |
10 |
|
+ |
1000.0 |
5 |
9 |
7 |
18 |
9 |
14 |
|
+ |
3333.3 |
9 |
8 |
9 |
7 |
10 |
9 |
|
+ |
10000.0 |
14 |
7 |
11 |
15 |
11 |
13 |
Compound |
Metabolic activation |
Dose (µg/plate) |
Histidine revertants/plate TA1538 |
||||||||
Experiment 1 |
Experiment 2 |
Experiment 3 |
|||||||||
|
|
Avrg |
|
|
Avrg |
|
|
Avrg |
|||
Negative control |
- |
|
28 |
28 |
29 |
19 |
14 |
16 |
25 |
18 |
22 |
- |
|
36 |
26 |
15 |
16 |
||||||
+ |
|
|
|
|
16 |
22 |
1 |
19 |
25 |
20 |
|
+ |
|
|
|
14 |
15 |
18 |
16 |
||||
Positive control |
|
|
|
|
|
|
|
|
|
|
|
2-Nitrofluorene |
- |
5.0 |
483 |
530 |
507 |
897 |
977 |
937 |
796 |
813 |
805 |
2-Anthramine |
- |
1.0 |
17 |
17 |
17 |
11 |
14 |
13 |
12 |
13 |
13 |
+ |
1.0 |
|
|
|
131 |
197 |
164 |
417 |
437 |
427 |
|
Manganese hypophosphite |
- |
33.0 |
16 |
8 |
12 |
10 |
9 |
10 |
|
|
|
- |
100.0 |
15 |
C* |
15 |
10 |
17 |
14 |
|
|
|
|
- |
333.3 |
17 |
19 |
18 |
13 |
13 |
13 |
|
|
|
|
- |
1000.0 |
30 |
26 |
28 |
8 |
9 |
8 |
|
|
|
|
- |
3333.3 |
25 |
21 |
23 |
10 |
9 |
10 |
|
|
|
|
- |
10000.0 |
32 |
20 |
26 |
13 |
7 |
10 |
|
|
|
|
+ |
33.0 |
C |
C |
|
8 |
7 |
8 |
21 |
16 |
19 |
|
+ |
100.0 |
C |
C |
|
12 |
23 |
18 |
10 |
28 |
19 |
|
+ |
333.3 |
C |
C |
|
26 |
17 |
22 |
20 |
18 |
19 |
|
+ |
1000.0 |
C |
C |
|
9 |
18 |
14 |
16 |
27 |
22 |
|
+ |
3333.3 |
C |
C |
|
20 |
13 |
17 |
22 |
13 |
18 |
|
+ |
10000.0 |
C |
C |
|
11 |
18 |
15 |
22 |
21 |
22 |
*C: contaminated
Compound |
Metabolic activation |
Dose (µg/plate) |
Histidine revertants/plate TA98 |
|||||
Experiment 1 |
Experiment 2 |
|||||||
|
|
Average |
|
|
Average |
|||
Negative control |
- |
|
68 |
48 |
51 |
40 |
33 |
38 |
- |
|
48 |
40 |
45 |
33 |
|||
+ |
|
43 |
31 |
41 |
42 |
60 |
48 |
|
+ |
|
38 |
51 |
40 |
51 |
|||
Positive control |
|
|
|
|
|
|
|
|
2- Nitrofluorene |
- |
5.0 |
356 |
452 |
404 |
418 |
524 |
471 |
2-Anthramine |
- |
1.0 |
39 |
25 |
32 |
48 |
35 |
42 |
+ |
1.0 |
178 |
219 |
198 |
212 |
206 |
209 |
|
Manganese hypophosphite |
- |
33.0 |
21 |
40 |
31 |
35 |
32 |
34 |
- |
100.0 |
30 |
34 |
32 |
30 |
35 |
33 |
|
- |
333.3 |
30 |
38 |
34 |
45 |
51 |
48 |
|
- |
1000.0 |
19 |
40 |
30 |
35 |
41 |
38 |
|
- |
3333.3 |
27 |
45 |
36 |
39 |
41 |
40 |
|
- |
10000.0 |
33 |
52 |
43 |
38 |
24 |
31 |
|
+ |
33.0 |
21 |
23 |
22 |
37 |
40 |
39 |
|
+ |
100.0 |
27 |
43 |
35 |
47 |
48 |
48 |
|
+ |
333.3 |
22 |
37 |
30 |
42 |
67 |
55 |
|
+ |
1000.0 |
33 |
28 |
31 |
40 |
51 |
46 |
|
+ |
3333.3 |
43 |
18 |
31 |
51 |
49 |
50 |
|
+ |
10000.0 |
C* |
C |
C |
39 |
43 |
41 |
*C: contaminated
Compound |
Metabolic activation |
Dose (µg/plate) |
Histidine revertants/plate TA100 |
|||||
Experiment 1 |
Experiment 2 |
|||||||
|
|
Average |
|
|
Average |
|||
Negative control |
- |
|
99 |
100 |
95 |
93 |
93 |
97 |
- |
|
100 |
81 |
84 |
117 |
|||
+ |
|
122 |
96 |
109 |
104 |
91 |
95 |
|
+ |
|
104 |
112 |
101 |
82 |
|||
Positive control |
|
|
|
|
|
|
|
|
Sodium azide |
- |
1.0 |
499 |
441 |
470 |
354 |
356 |
355 |
2-Anthramine |
- |
1.0 |
108 |
109 |
109 |
102 |
120 |
111 |
+ |
1.0 |
349 |
352 |
351 |
371 |
405 |
388 |
|
Manganese hypophosphite |
- |
0.3 |
116 |
98 |
107 |
35 |
32 |
34 |
- |
3.3 |
108 |
98 |
98 |
30 |
35 |
33 |
|
- |
33.3 |
92 |
101 |
97 |
102 |
105 |
104 |
|
- |
100.0 |
113 |
111 |
112 |
112 |
97 |
105 |
|
- |
333.3 |
136 |
100 |
118 |
99 |
104 |
102 |
|
- |
1000.0 |
91 |
109 |
100 |
105 |
98 |
102 |
|
- |
3333.3 |
93 |
93 |
93 |
126 |
125 |
126 |
|
- |
10000.0 |
108 |
108 |
108 |
108 |
100 |
104 |
|
+ |
0.3 |
122 |
88 |
105 |
|
|
|
|
+ |
3.3 |
101 |
97 |
99 |
|
|
|
|
+ |
33.3 |
90 |
105 |
98 |
104 |
102 |
103 |
|
+ |
100.0 |
105 |
101 |
103 |
110 |
115 |
113 |
|
+ |
333.3 |
92 |
92 |
92 |
86 |
120 |
103 |
|
+ |
1000.0 |
101 |
72 |
87 |
109 |
104 |
107 |
|
+ |
3333.3 |
87 |
81 |
84 |
115 |
101 |
108 |
|
+ |
10000.0 |
90 |
84 |
87 |
114 |
125 |
120 |
Compound |
Metabolic activation |
Dose (µg/plate) |
Histidine revertants/plate E. coli WP2 |
|||||
Experiment 1 |
Experiment 2 |
|||||||
|
|
Average |
|
|
Average |
|||
Negative control |
- |
|
20 |
21 |
26 |
41 |
49 |
48 |
- |
|
32 |
31 |
50 |
50 |
|||
+ |
|
45 |
49 |
47 |
50 |
48 |
52 |
|
+ |
|
53 |
42 |
72 |
39 |
|||
Positive control |
|
|
|
|
|
|
|
|
AF-2 |
- |
0.1 |
516 |
574 |
545 |
146 |
152 |
149 |
2-Anthramine |
- |
10.0 |
27 |
24 |
26 |
18 |
30 |
24 |
+ |
10.0 |
605 |
662 |
634 |
263 |
285 |
274 |
|
Manganese hypophosphite |
- |
33.3 |
29 |
24 |
27 |
30 |
39 |
35 |
- |
100.0 |
32 |
27 |
30 |
42 |
38 |
40 |
|
- |
333.3 |
33 |
32 |
33 |
39 |
45 |
42 |
|
- |
1000.0 |
21 |
26 |
24 |
61 |
40 |
51 |
|
- |
3333.3 |
41 |
37 |
39 |
43 |
48 |
46 |
|
- |
10000.0 |
37 |
32 |
35 |
52 |
52 |
52 |
|
+ |
33.3 |
41 |
34 |
38 |
51 |
60 |
56 |
|
+ |
100.0 |
42 |
48 |
45 |
64 |
64 |
64 |
|
+ |
333.3 |
52 |
49 |
51 |
52 |
52 |
52 |
|
+ |
1000.0 |
39 |
51 |
45 |
26 |
62 |
44 |
|
+ |
3333.3 |
33 |
68 |
51 |
65 |
77 |
71 |
|
+ |
10000.0 |
49 |
65 |
57 |
89 |
82 |
86 |
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Gene mutation in vitro:
Various data available for the target chemical was reviewed to determine the mutagenic nature of Manganese hypophosphite. The studies are as mentioned below:
Gene mutation toxicity study was performed by to determine the mutagenic nature of target chemical and its structurally and functionally similar read across chemical . The study was performed as per the plate incorporation protocol and the test chemical dissolved in 0.067M potassium or sodium phosphate buffer and was used at dose levels of 0, 0.033, 0.10, 0.33, 1.0, 3.3 and 10 mg per plate. Concurrent solvent and positive controls were run with the test chemical. Test results were considered valid only if the positive control compounds induced increases in mutant counts to at least twice background. The target and the test chemical did not induce mutation in the Salmonella typhimurium strain TA98, TA100, TA1535, TA1537 and TA1538 and Escherichia coli strain WP2 with and without rat liver S9 mix and hence the two chemicals are negative for gene mutation in vitro.
In another study for structurally and functionally similar read across chemical (RA CAS no 7664 -38 -2), Gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical. The study was performed by the standard plate incorporation method. The chemical was dissolved in distilled water as the solvent and used at dose levels of 0, 0.5, 1.00 or 2.00µL/plate using Salmonella typhimurium strainTA97, TA98, TA100 and TA104 with and without S9 metabolic activation system. The spontaneous reversions of the tester strains were within the acceptable range and a decreased spontaneous reversion frequency of TA97 was noted. In the presence or absence of S9 mix, the numbers of revertants in all tester strains for all concentrations of the acids tested were not significantly different from the respective negative controls.Phosphoric acid did not induce gene mutation in Salmonella typhimurium strainTA97, TA98, TA100 and TA104 in the presence and absence of S9 metabolic activation system and hence is not likely to classify as a gene mutant in vitro.
Based on the data available for the target chemical and its two read across chemicals, Manganese hypophosphite does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant in vitro.
Justification for classification or non-classification
Based on the data available for the target chemical and its two read across chemicals, Manganese hypophosphite (CAS no 10043 -84 -2) does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant in vitro.
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