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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From Jun. 09, 1986 to Jun. 11, 1986
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Controls: Yes, control and solvent control
- Chemical name of vehicle (organic solvent): Acetone
- Concentration of stock solution and final test solution: Working standard/Stock solution: 100 mg/mL; Final test solution: 1, 1.8, 3.2, 5.6 and 10 mg/mL
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Source: ABC in-house culture
- Age at study initiation: 24 h

ACCLIMATION
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Algae (Selenastrum capricornutum) supplemented with a suspension of fish food
- Feeding frequency: Once in three days
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
225-275 ppm (as CaCO3)
Test temperature:
20 ± 2 °C
pH:
8.2-8.3
Dissolved oxygen:
8.2-8.9 mg/L
Salinity:
No data
Nominal and measured concentrations:
Nominal concentrations:
- Range finding test: 1.0, 10 and 100 mg/L
- Definitive test: 0 (control and solvent control), 1.0, 1.8, 3.2, 5.6 and 10 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beakers
- Type: Open
- Material, size, headspace, fill volume: 250 mL glass beakers containing 200 mL of ABC aged well water
- No. of organisms per vessel: Ten
- No. of vessels per concentration (replicates): Two


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Well water
- Dissolved oxygen: 9.2-10.1 ppm
- Total alkalinity: 325- 375 ppm as CaCO3
- pH: 7.8- 8.3
- Pesticides: < 0.10 and < 0.50 ppb for organophosphorous and organochlorine pesticides respectively
- Conductivity: 700 µ mhos/cm


OTHER TEST CONDITIONS
- Photoperiod: 16 h day light with 30-minute simulated dawn and dusk periods
- Light intensity: 50-70 foot candles
- Water quality parameters of temperature, dissolved oxygen and pH were measured at the termination of the test and were within acceptable limits


TEST CONCENTRATIONS
- Spacing factor for test concentrations: Logarithmic series
- Range finding study
- Test concentrations: 1, 10 and 100 mg/L
Reference substance (positive control):
no
Key result
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
6.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 5.6- 10
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
5.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: (e.g. 95 % CL) 4.3- 6.5
Key result
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
< 1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks:
and abnormal effects
Remarks on result:
other: -
Details on results:
- Mortality of control: Single mortality in the control, considered aberrant
- Abnormal responses: Quiescence, surfacing and/or daphnids lying on the bottom of test vessels were observed at all test concentrations
Results with reference substance (positive control):
No data
Reported statistics and error estimates:
Statistical analysis was performed using a computerized LC50 program using the binomial, moving average angle and probit methods.
Validity criteria fulfilled:
yes
Conclusions:
Under the test conditions, the 48 h LC50 of the test material to Daphnia magna was determined to be 5.2 mg/L (95% C.L.: 4.3-6.5 mg/L). The NOEC was estimated to be < 1.0 mg/L, the lowest dose tested.
Executive summary:

A study was performed to evaluate the acute toxicity of the test material to Daphnia magna under static conditions. The study was conducted according to a method equivalent or similar to OECD Guideline 202, in compliance with GLP. Daphnia magna were exposed to the test substance at concentrations of 0, 1.0, 1.8, 3.2, 5.6 and 10 mg/L. Mortality and abnormal effects were determined at 24 and 48 h. Mortality, quiescence, surfacing and/or daphnids lying on the bottom of test vessels were observed at all test concentrations. The single mortality in the control was considered aberrant. Under the test conditions, the 48 h LC50 of the test substance to Daphnia magna was determined to be 5.2 mg/L (95% C.L.: 4.3-6.5 mg/L). The NOEC was estimated to be <1.0 mg/L, the lowest dose tested (Schoen, 1986).

Description of key information

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
5.2 mg/L

Additional information

A study was performed to evaluate the acute toxicity of the test material to Daphnia magna under static conditions. The study was conducted according to a method equivalent or similar to OECD Guideline 202, in compliance with GLP. Daphnia magna were exposed to the test substance at concentrations of 0, 1.0, 1.8, 3.2, 5.6 and 10 mg/L. Mortality and abnormal effects were determined at 24 and 48 h. Mortality, quiescence, surfacing and/or daphnids lying on the bottom of test vessels were observed at all test concentrations. The single mortality in the control was considered aberrant. Under the test conditions, the 48 h LC50 of the test substance to Daphnia magna was determined to be 5.2 mg/L (95% C.L.: 4.3-6.5 mg/L). The NOEC was estimated to be <1.0 mg/L, the lowest dose tested (Schoen, 1986).