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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From Jan. 06, 1987 to Jan. 10, 1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
yes
Remarks:
ErC50 not reported
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Controls: Yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Strain: Selenastrum capricornutum, Strain no. 22662
- Source (laboratory, culture collection): American Type Culture Collection


ACCLIMATION
- Culturing media and conditions (same as test or not): Sterile OECD Algal Medium
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
No data
Test temperature:
21-25 ± 2 °C
pH:
6.4-6.7
Dissolved oxygen:
No data
Salinity:
No data
Nominal and measured concentrations:
0, 0.32, 1, 3.2 and 10 ppm (mg/mL)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): Closed
- Material, size, headspace, fill volume: Erlenmeyer flasks, 250 mL flask, capped with inverted glass beakers
- Initial cells density: 10,000 (for insoluble test material)
- No. of vessels per concentration (replicates): Three
- No. of vessels per control (replicates): Three
- No. of vessels per vehicle control (replicates): Three


GROWTH MEDIUM
- Standard medium used: Yes, fresh Water Algal Culture Medium (OECD)


OTHER TEST CONDITIONS
- Sterile test conditions: Yes
- Light intensity and quality: 8000 Lux, cool-white flourescent bulbs


TEST CONCENTRATIONS
- Range finding study
- Test concentrations: No data
- Results used to determine the conditions for the definitive study: Yes
Reference substance (positive control):
not specified
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
6.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: -
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
1.36 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: -
Key result
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
2.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: -
Key result
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
0.34
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: -
Details on results:
- Insufficient cell growth was observed at 24 and 48 h for all concentrations and for the blank control. The median effects, therefore, could not be calculated for these time periods.
Reported statistics and error estimates:
No data
Validity criteria fulfilled:
yes
Conclusions:
Under the test conditions, the EbC50 of the test material to Selenastrum capricornutum at 72 and 96 h were estimated to be 6.9 and 2.1 mg/L, respectively. The NOEC at 72 and 96 h were 1.36 and 0.34 mg/L, respectively.
Executive summary:

A study was performed to evaluate the toxicity of the test substance to Selenastrum capricornutum under static conditions according to OECD Guideline 201, in compliance with GLP. The algae were exposed at 0, 0.32, 1.0, 3.2 and 10 mg/L for 72 h. Growth inhibition was measured at 24, 48 and 72 h. Insufficient cell growth was observed at 24 and 48 h for all concentrations and for the blank control. The median effects, therefore, could not be calculated for these time periods. Under the test conditions, the EbC50 of the test substance to Selenastrum capricornutum at 72 and 96 h were estimated to be 6.9 and 2.1 mg/L, respectively. The NOEC at 72 and 96 h were found to be 1.36 and 0.34 mg/L, respectively (Drozdowski, 1987).

Description of key information

Key value for chemical safety assessment

EC50 for freshwater algae:
2.1 mg/L
EC10 or NOEC for freshwater algae:
0.34 mg/L

Additional information

A study was performed to evaluate the toxicity of the test substance to Selenastrum capricornutum under static conditions according to OECD Guideline 201, in compliance with GLP. The algae were exposed at 0, 0.32, 1.0, 3.2 and 10 mg/L for 72 h. Growth inhibition was measured at 24, 48 and 72 h. Insufficient cell growth was observed at 24 and 48 h for all concentrations and for the blank control. The median effects, therefore, could not be calculated for these time periods. Under the test conditions, the EbC50 of the test substance to Selenastrum capricornutum at 72 and 96 h were estimated to be 6.9 and 2.1 mg/L, respectively. The NOEC at 72 and 96 h were found to be 1.36 and 0.34 mg/L, respectively (Drozdowski, 1987).