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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study; no deviations

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid: viscous
Details on test material:
- Name of test material (as cited in study report):Vinylpseudoionol
- Molecular formula (if other than submission substance): C15H24O
- Molecular weight (if other than submission substance): 220.36 g/mol
- Physical state: viscous-liquid
- Analytical purity: 87.1 %
- Impurities (identity and concentrations): 1.2 % water
- Purity test date: not indicated
- Lot/batch No.: technikumscharge M 401
- Expiration date of the lot/batch: not indicated
- Storage condition of test material: storage at room temperature under nitrogen
- Water solubility: poorly soluble

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
sewage, domestic (adaptation not specified)
Details on inoculum:
Activated sludge from laboratory wastewater plants treating municipal sewage . Concentration of dry substance
30 mg/L
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
25 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
other: TOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium:
- Additional substrate: no
- Test temperature: 20 - 25 °C
- pH: 7.0 - 7.4
- pH adjusted: yes
- Aeration of dilution water: yes (CO2 free air)
- Suspended solids concentration: 7.5 ml/L inoculum (6 g/l dry matter)
- Continuous darkness: yes/no
- Other:

TEST SYSTEM
- Culturing apparatus: brown glass bottles
- Number of culture flasks/concentration: 2 per test concentration, 1 per controls
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: CO2 trap according to OECD 301 B


SAMPLING
- Sampling days: 0, 1, 3, 5, 7, 10, 12 14, 17, 21, 24, 27, 29
- Sample storage before analysis: not relevant as the samples were analysed asap after sampling


CONTROL AND BLANK SYSTEM
- Inoculum blank: 1
- Abiotic sterile control: 1
- Toxicity control: 1


Reference substance
Reference substance:
aniline

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (CO2 evolution)
Value:
0 - 10
Sampling time:
3 d
Parameter:
% degradation (CO2 evolution)
Value:
10 - 20
Sampling time:
7 d
Parameter:
% degradation (CO2 evolution)
Value:
60
Sampling time:
12 d
Parameter:
% degradation (CO2 evolution)
Value:
60 - 70
Sampling time:
14 d
Parameter:
% degradation (CO2 evolution)
Value:
90 - 100
Sampling time:
27 d

BOD5 / COD results

Results with reference substance:
day 0: 0 %
day 3: -1 %
day 5: 27 %
day 14: 60 - 70 %
day 28: 70 - 80 %

Applicant's summary and conclusion