Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study, no deviations

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report Date:
1999

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid: viscous
Details on test material:
- Name of test material (as cited in study report): Vinylpseudoionol
- Molecular formula (if other than submission substance): C15H24O
- Molecular weight (if other than submission substance): 220.36 g/mol
- Physical state: viscous liquid
- Analytical purity: 87.1 %
- Impurities (identity and concentrations): 1.2 % water
- Purity test date: not indicated
- Lot/batch No.: Technikumscharge M401
- Expiration date of the lot/batch: not indicared
- Storage condition of test material: ca. 4 °C under nitrogen
- Water solubility: poorly water soluble

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
Inoculum:
Activated sludge from laboratory wastewater plants treating municipal sewage Concentration of dry substanec 1 g/L

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
30 min

Test conditions

Test temperature:
20 +/- 2 °C
pH:
6.3 - 6.5 before correction; 7.2 - 7.4 after correction
Dissolved oxygen:
> 2.5 mg/l during aeration; > 6.5 mg/L before measurement
Nominal and measured concentrations:
nominal: 0, 100 mg/L
Details on test conditions:
Preparation of the stock solution of the test substance: - none , direct addition
Preparation of the inoculum:
The inoculum was washed, brought to a concentration of 5 g/I dry substance and aerated for 24 hours. 50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test.

TEST SYSTEM
- Test vessel: Erleenmeyer vessel (nominal 250 ml)
- Material, size, headspace, fill volume: 250 ml
- Aeration: yes
- No. of organisms per vessel: 5 g/L dry substance
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 209

OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: not relevant
- Light intensity: not relevant

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : 30, oxygen consumption

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test)
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC20: ca. 3.3 mg/L
EC50: ca. 20 mg/L

Applicant's summary and conclusion