Fatty acids, C16-18 and C18-hydroxy, oligomeric reaction products with adipic acid, decanoic acid, isooctadecanoic acid, octanoic acid, pentaerythritol and stearic acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 - 15 Dec 2006

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study: Analytical purity is not stated. Age of test organisms is not given. Only two replicates are used. No analytics of the test substance was performed.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

no

Remarks:

But conducted in accordance with ISO/IEC 17025

Test material

Test material information

Constituent 1

Reference substance name:

Fatty acids, C8-10, mixed esters with adipic acid and trimethylolpropane

EC Number:

306-085-3

EC Name:

Fatty acids, C8-10, mixed esters with adipic acid and trimethylolpropane

Cas Number:

95912-89-3

IUPAC Name:

95912-89-3

Details on test material:

- Name of test material: Only trade name is given.
- Analytical purity: not stated
- Lot/batch No.: 10920

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Water Accomodated fraction was prepared by moderately stirring 100 mg test substance per litre of medium overnight followed by filtration (Millipore AP 15 glass fiber filter).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Age at study initiation: not stated
- Length at study initiation: < 2 mm
- Method of breeding: Parental and young Daphnia were held in 40 L glass aquaria, which contain continuously aerated reconstituted water according to ISO 6341 prepared with distilled water. The population were fed once a day (except weekends) with 15 g sera microspan (sera GmbH, 52525 Heinsberg, Germany) homogenized in 1 L deionized water. Every month, 25% of the total volume of the aquaria is replaced by fresh water.
- Feeding during test: no

ACCLIMATION
- Acclimation period: 4 h
- Acclimation conditions: same as test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

20 ± 0.5 °C

pH:

7.3 - 7.6

Dissolved oxygen:

5.6 - 6.0 mg/L

Nominal and measured concentrations:

Nominal: 100 mg/L loading rate WAF

Details on test conditions:

TEST SYSTEM
- Test vessel: flask
- Material:glass; Size: 100 mL; Fill volume: 50 mL test medium
- Aeration: No
- No. of organisms per vessel: 20
- No. of vessels per concentration: 2
- No. of vessels per control: 3

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: distilled water
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
- Conductivity: < 1.5 µS/cm
- Culture medium different from test medium: No, reconstituted water according to ISO 6341.
- Intervals of water quality measurement: Temperature was measured in a control flask at the start and at the end of the test. Oxygen and pH were measured in all vessels prior to the addition of Daphnia to the test medium and at the end of the test.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h photoperiod a day
- Light intensity: overhead white fluorescent light.

EFFECT PARAMETERS MEASURED: Immobilisation was recorded after 24 and 48 h exposure time.

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: limit test
- Range finding study: This study represents a limit test. Therefore, no range finding test was conducted.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

(WAF)

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EL50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

(WAF)

Basis for effect:

mobility

Any other information on results incl. tables

Table 1: Immobilisation of Daphnia magna after 24 and 48 h of exposure to the test material

Nominal concentration [mg/L]	Code	Number of individuals per test vessel	Immobile Daphnia [Ind./vessel] 24 h	Immobile Daphnia [Ind./vessel] 48 h	% immobile Daphnia after 24 h of exposure	% immobile Daphnia after 48 h of exposure
Control	A	20	0	0	0	0
	B	20	0	0	0	0
	C	20	0	0	0	0
100	A	20	0	1	0	5
	B	20	0	0	0	0