Fatty acids, C16-18 and C18-hydroxy, oligomeric reaction products with adipic acid, decanoic acid, isooctadecanoic acid, octanoic acid, pentaerythritol and stearic acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May - June 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Fatty acids, C16-18 and C18-hydroxy, oligomeric reaction products with adipic acid, decanoic acid, isooctadecanoic acid, octanoic acid, pentaerythritol and stearic acid

EC Number:

500-320-5

EC Name:

Fatty acids, C16-18 and C18-hydroxy, oligomeric reaction products with adipic acid, decanoic acid, isooctadecanoic acid, octanoic acid, pentaerythritol and stearic acid

Cas Number:

130353-58-1

Molecular formula:

not applicable, the substance is UVCB

IUPAC Name:

1,6-bis({2-[(hexadecanoyloxy)methyl]-3-hydroxy-2-(hydroxymethyl)propyl}) hexanedioate; 1-{2-[(decanoyloxy)methyl]-3-hydroxy-2-[(octadecanoyloxy)methyl]propyl} 6-[3-hydroxy-2-(hydroxymethyl)-2-{[(3-methylheptadecanoyl)oxy]methyl}propyl] hexanedioate; 3-hydroxy-2-{[(6-{[3-hydroxy-2-(hydroxymethyl)-2-[(octadecanoyloxy)methyl]propyl]peroxy}-6-oxohexanoyl)oxy]methyl}-2-(hydroxymethyl)propyl 12-hydroxyoctadecanoate

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Activated sludge was obtained from Totnes Sewage Treatment Works, Totnes, Devon, UK. This works treats sewage of predominantly domestic origin.
- Storage conditions: kept at room temperature at pH 7.0 ± 1.0
- Preparation of inoculum for exposure: Seven days prior to the exposure start the activated sludge was centrifuged, washed and re-suspended in the mineral medium.
- Concentration of sludge:
- Initial cell/biomass concentration:
- Water filtered: yes/no
- Type and size of filter used, if any:

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

100 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

2.36 other: g O2/g

Based on:

COD

Initial test substance concentration 3

Initial conc.:

1.67 other: g O2/g

Based on:

ThOD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: 0.25 mg/L KH2PO4, 22.5 mg/L K2HPO4, 36.4 mg/L Na2HPO4.2H2O, 5.0 mg/L NH4Cl, 334.0 mg/L CaCl2.2H2O, 217.5 mg/L MgSO4.7H2O, 85.0 mg/L FeCl3.6H2O
- Test temperature: 21 ±1°C
- pH: 7.4 ± 0.2 (test start), 7.6 to 7.8 (day 28)
- CEC (meq/100 g):
- Suspended solids concentration: 30 mg/L
- Continuous darkness: no


TEST SYSTEM
- Culturing apparatus: Oxitop respirometers (Wissenschaftlich-Technische Werkstätten, GmbH, Germany)
- Number of culture flasks/concentration: 3
- Measuring equipment: cuvette tests provided by Hach Lange Ltd using a DR2800 spectrophotometer


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

65

Sampling time:

28 d

Details on results:

The ten day window criterion was not met. As The test substance is an UVCB the ten day window is not relevant for the substance.

BOD5 / COD results

Results with reference substance:

Biodegradation of reference substance: 73% after 14 d

Any other information on results incl. tables

The mean toxicity control degradation achieved on day 14 was 38% (based on ThOD and COD), as this is > 25% the test substance is assumed not to be inhibitory at this concentration.

Applicant's summary and conclusion

Interpretation of results:

readily biodegradable, but failing 10-day window

Conclusions:

As the substance is an UVCB the 10-day window is not relvant