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EC number: 221-717-7 | CAS number: 3209-22-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- other: peer-reviewed data from OECD SIDS 2002
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Vehicle:
- yes
- Remarks:
- DMSO: HCO-40 = 9:1
- Test organisms (species):
- Daphnia magna
- Total exposure duration:
- 21 d
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.06 mg/L
- Conc. based on:
- not specified
- Basis for effect:
- not specified
- Remarks on result:
- other: reported as ppm w/v
- Duration:
- 21 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.06 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- not specified
- Remarks on result:
- other: reported as ppm w/v
- Validity criteria fulfilled:
- not specified
- Executive summary:
No observed effect concentration (maximum) for 21 days: NOEC< 0.06 mg/L (w/v). (Reported as ppm w/v).
First (lowest) observed effect concentration (minimum) for 21 days LOEC= 0.06 mg/L (w/v). (Reported as ppm w/v).
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Principles of method if other than guideline:
- Method: other: UBA-Verfahrensvorschlag (vorläeufiger) "Verläengerter Toxizitäetstest bei Daphnia magna" (Bestimmung der NOEC fuer Reproduktionsrate, Mortalitaet und den Zeitpunkt des ersten Auftretens von Nachkommen; 21d) (01.02.1984)
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Test organisms (species):
- Daphnia magna
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Validity criteria fulfilled:
- not specified
- Executive summary:
Kühn, 1989
In a 21 d toxicity test, a NOEC of >0.1 mg/L (highest tested concentration) was determined for Daphnia magna.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- other: applicant's summary entry
- Adequacy of study:
- other information
- Reliability:
- other: applicant's summary entry
- Rationale for reliability incl. deficiencies:
- other: No reliability is given as this is an applicant's summary entry for a variety of studies.
- Principles of method if other than guideline:
- applicant's summary entry
- GLP compliance:
- not specified
- Validity criteria fulfilled:
- not specified
Referenceopen allclose all
highest conc. tested; nominal conc.
Deneer et al., 1989
Rel. 2: National guideline study. Basic data given.
Method:
A 21-day IC50 value for Daphnia magna was determined following the NEN 6502 (1980) semi-static procedure, feeding the daphnids 3x10E8 cells/l/d of the green algae Chlorella pyrenoidosa. Test concentrations increased geometrically with a factor of 3.2. IC50 values were calculated according to Kooyman (1981) on the basis of added amounts of test material. The length after 21 days of exposure of all surviving parent daphnids was determined using a binocular equipped with an oculary micrometer. The length of the animals was measured from the top of the head to the base of the caudal spine.
The population growth constant (rm) of Daphnia magna was determined in a semi-static test over a 21-day period as outlined by Van Leeuwen et al., (1985), but using 10 daphnids per concentration, and one animal per jar containing 50 ml medium.
The concentration causing a significant (P<0.01) lowering of rmand length between treatments and control (LRCT(rm) and LRCT(length), respectively) was determined using Williams procedure (Williams, 1971, 1972).
All tests with Daphna magna were carried out in rooms at 20 ± 0.5°C, which were illuminated 12 h/day. A synthetic test medium was used (NPR 6503,1980) with a hardness of 200 mg/l as CaCO3 and a pH of 8.4 ± 0.1. The medium was saturated with air prior to use. All daphnids used were < 24 h old at the start of the experiment. Mortality of daphnids in controls never exceeded 10%.
Results:
21-day IC50 = 3.5 mg/l, based on mobility
21-day LRCT(rm) = 1.8 mg/l (=> lowest concentration tested that significantly degreased the intrinsic growth rate (rm) of D. magna populations)
21-day LRCT(length) = 1.0 mg/l (=> lowest concentration tested that significantly degreased the length of D. magna after 21 days of exposure)
Maas-Diepeveen and van Leeuwen, 1986
Rel. 2: National guideline study. Basic data given.
Method:
In order to consider effects on reproduction and growth of 2,3-dichloronitrobenzene, long-term experiments were carried out on Daphnia magna. In order to determine effects on the intrinsic rate of natural increase (rm) of D. magna, chronic tests were conducted as described by Van Leeuwen et al. (1985). For each concentration, however, 10 daphnids were used, one animal per jar containing 50 ml medium. 21 -day LC50 experiments were carried out according to NEN 6502 (1980), using a food concentration of 3 x 10E8 Chlorella pyrenoidosa cells/l/day. LC50 values were determined using the method described by Kooyman (1981). The mean size of daphnids was determined from treatments and controls and differences were tested using Williams procedure (1971, 1972). 2,3-dichloronitrobenzene used in the test had a purity of >98%. The stock solution was prepared in dimethylsulfoxide (DMSO; Merck, purity 99%).
Results:
21-day LC50 = 3.5 mg/l (95%C.L. 3.4-3.6)
21-day LRCT(rm) = 1.8 mg/l
21-day LRCT(length) = 1.0 mg/l
Description of key information
For transported isolated intermediates according to Reach, Annex XVIII, this endpoint is not a data requirement. However, data is available for this endpoint and is thus reported under the guidance of "all available data".
Deneer et al., 1989
Rel. 2: National guideline study. Basic data given.
Method:
A 21-day IC50 value for Daphnia magna was determined following the NEN 6502 (1980) semi-static procedure, feeding the daphnids 3x10E8 cells/l/d of the green algae Chlorella pyrenoidosa. Test concentrations increased geometrically with a factor of 3.2. IC50 values were calculated according to Kooyman (1981) on the basis of added amounts of test material. The length after 21 days of exposure of all surviving parent daphnids was determined using a binocular equipped with an oculary micrometer. The length of the animals was measured from the top of the head to the base of the caudal spine.
The population growth constant (rm) of Daphnia magna was determined in a semi-static test over a 21-day period as outlined by Van Leeuwen et al., (1985), but using 10 daphnids per concentration, and one animal per jar containing 50 ml medium.
The concentration causing a significant (P<0.01) lowering of rmand length between treatments and control (LRCT(rm) and LRCT(length), respectively) was determined using Williams procedure (Williams, 1971, 1972).
All tests with Daphna magna were carried out in rooms at 20 ± 0.5°C, which were illuminated 12 h/day. A synthetic test medium was used (NPR 6503,1980) with a hardness of 200 mg/l as CaCO3 and a pH of 8.4 ± 0.1. The medium was saturated with air prior to use. All daphnids used were < 24 h old at the start of the experiment. Mortality of daphnids in controls never exceeded 10%.
Results:
21-day IC50 = 3.5 mg/l, based on mobility
21-day LRCT(rm) = 1.8 mg/l (=> lowest concentration tested that significantly degreased the intrinsic growth rate (rm) of D. magna populations)
21-day LRCT(length) = 1.0 mg/l (=> lowest concentration tested that significantly degreased the length of D. magna after 21 days of exposure)
Maas-Diepeveen and van Leeuwen, 1986
Rel. 2: National guideline study. Basic data given.
Method:
In order to consider effects on reproduction and growth of 2,3-dichloronitrobenzene, long-term experiments were carried out on Daphnia magna. In order to determine effects on the intrinsic rate of natural increase (rm) of D. magna, chronic tests were conducted as described by Van Leeuwen et al. (1985). For each concentration, however, 10 daphnids were used, one animal per jar containing 50 ml medium. 21 -day LC50 experiments were carried out according to NEN 6502 (1980), using a food concentration of 3 x 10E8 Chlorella pyrenoidosa cells/l/day. LC50 values were determined using the method described by Kooyman (1981). The mean size of daphnids was determined from treatments and controls and differences were tested using Williams procedure (1971, 1972). 2,3-dichloronitrobenzene used in the test had a purity of >98%. The stock solution was prepared in dimethylsulfoxide (DMSO; Merck, purity 99%).
Results:
21-day LC50 = 3.5 mg/l (95%C.L. 3.4-3.6)
21-day LRCT(rm) = 1.8 mg/l
21-day LRCT(length) = 1.0 mg/l
Kühn, 1989
In a 21 d toxicity test, a NOEC of >0.1 mg/L (highest tested concentration) was determined for Daphnia magna.
OECD SIDS, 2002
Results:
21-day NOEC <0.06 mg/L
21-day LOEC =0.06 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- mg/L
Additional information
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